Paper · 2017 · OENO One

Brassinosteroids and gibberellic acid: effects on in vitro pollen germination in grapevine

Zeliha Gokbayrak; Hakan Engin

3
claims
7
verbatim quotes
10
citations
2
research areas
✓
Open access · cc-by

Abstract

Many physiological processes related to plant growth and development are under the influence of growth regulators, which also have an impact on pollen germination. In this study, the effects of two brassinosteroid compounds, epibrassinolide and 22S,23S-homobrassinolide, and gibberellic acid (GA3) on in vitro pollen germination of two table grape cultivars, ‘Italia’ and ‘Cardinal’ (Vitis vinifera L.), were determined. A total of 28 treatments, alone and in combination, were applied to freshly collected pollens which were sown on a basic medium with 1% agar and 20% sucrose. Petri dishes were kept at 26±1°C for 24 hours. Counting of the germinated pollens revealed that the effects of these plant hormones were cultivar- and substance-specific. The cultivar ‘Italia’ was not influenced by the treatments (the highest germination ratio being 44.4% from 0.001 mg L-1 epibrassinolide) as opposed to the cultivar ‘Cardinal’. The highest germination ratio in ‘Cardinal’ was about 50% in pollens treated with 25 mg L-1 GA3 + 0.01 mg L-1 epibrassinolide. The control group resulted in 32.38% germination. Combining GA3 with epibrassinolide provided slightly higher germination ratios compared to combining GA3 with 22S,23S-homobrassinolide.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 3

Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Pollen germination of cultivar Italia was not influenced by the treatments, with a highest germination ratio of 44.4% from epibrassinolide.

Taxon
Vitis vinifera L.Vitis viniferae0
Material
Italiae1
Treatment
0.001 mg L-1 epibrassinolidee1
Outcome
germination ratioe1
Direction
not influencede1
Value
44.4%e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis vinifera
Vitis vinifera · TaxonDarijanus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two table grape cultivars, ‘Italia’ and ‘Cardinal’ (Vitis vinifera L.)”

✓ located verbatim in the source · abstract, characters 332–402 · show in text ↑
e1

“The cultivar ‘Italia’ was not influenced by the treatments (the highest germination ratio being 44.4% from 0.001 mg L-1 epibrassinolide) as opposed to the cultivar ‘Cardinal’”

✓ located verbatim in the source · abstract, characters 750–924 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pollen of cultivar Cardinal treated with 25 mg L-1 GA3 and 0.01 mg L-1 epibrassinolide had the highest germination ratio, about 50%, against 32.38% in the control.

Taxon
Vitis vinifera L.Vitis viniferae0
Material
Cardinale2
Treatment
pollens treated with 25 mg L-1 GA3 + 0.01 mg L-1 epibrassinolidee2
Outcome
germination ratioe2
Direction
higheste2
Value
about 50%e2
Comparator
control groupe3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis vinifera
Vitis vinifera · TaxonDarijanus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“two table grape cultivars, ‘Italia’ and ‘Cardinal’ (Vitis vinifera L.)”

✓ located verbatim in the source · abstract, characters 332–402 · show in text ↑
e2

“The highest germination ratio in ‘Cardinal’ was about 50% in pollens treated with 25 mg L-1 GA3 + 0.01 mg L-1 epibrassinolide”

✓ located verbatim in the source · abstract, characters 926–1051 · show in text ↑
e3

“The control group resulted in 32.38% germination”

✓ located verbatim in the source · abstract, characters 1053–1101 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combining GA3 with epibrassinolide gave slightly higher pollen germination ratios than combining GA3 with 22S,23S-homobrassinolide in grapevine.

Taxon
grapevineVitist0
Treatment
Combining GA3 with epibrassinolidee4
Outcome
germination ratiose4
Direction
slightly highere4
Comparator
combining GA3 with 22S,23S-homobrassinolidee4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

t0

“pollen germination in grapevine”

✓ located verbatim in the source · title, characters 59–90 · show in text ↑
e4

“Combining GA3 with epibrassinolide provided slightly higher germination ratios compared to combining GA3 with 22S,23S-homobrassinolide”

✓ located verbatim in the source · abstract, characters 1103–1237 · show in text ↑

Gibberellic acid · growth · VitisResults differ

34 papers: 26 positive, 8 negative or no effect

25 papers in the same direction as this paper · 8 in the opposite direction

  1. negative
  2. negative
  3. negative

30 papers more in this group

See all 34 papers →

24-epi-brassinolide · growth · VitisPositive and negative results

4 papers: 3 positive, 1 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive
See all 4 papers →

Gibberellic acid · growth · Vitis vinifera (wine grape)Positive and negative results

10 papers: 8 positive, 2 negative or no effect

7 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. no effect
  3. positive

6 papers more in this group

See all 10 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.