Abstract
An experiment was performed as a split-plot design based on a randomized complete block (RCB) with three replicates. The aim was to explore physiological traits, catalase (CAT) and peroxidase (POD) activity associated with cold and freeze stress in four barley genotypes (two stress-tolerant genotypes and two sensitive genotypes). The genotypes were regarded as sub-plots and five temperature levels (namely 20, 4, 0, −4, −8°C) as main plots. The results revealed that the hydrogen peroxide (H2O2) and malondialdehyde (MDA) content increased through freeze and cold stress from 20 to 4°C. This increase was more in the sensitive genotypes than in the tolerant ones. Subsequent to the increase in lipid peroxidation, the membrane stability index (MSI) decreased, thereby increasing the electrolyte leakage. The sensitive genotypes demonstrated greater reduction in the studied parameters. Upon the increase in the H2O2 content, the antioxidant enzymes CAT and POD also increased in order to collect and prevent H2O2 from damaging the cell and the plant. Compared with the sensitive ones, the tolerant genotypes indicated more increase in the enzymes. The H2O2 and MDA content decreased from −4 to −8°C, which could be due to intracellular freezing in the sensitive genotypes, but due to the detoxifying of the antioxidant enzymes in the tolerant genotypes. Compared with the POD content, the increase in the CAT content through cold stress was more, which could mean that it was possibly CAT which acted as the main inhibitor of H2O2 in cold stress in the barley plant.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Cold and freeze stress increased hydrogen peroxide and malondialdehyde content from 20 to 4°C.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- freeze and cold stresse0
- Outcome
- hydrogen peroxide (H2O2) and malondialdehyde (MDA) contente0
- Direction
- increasede0
- Comparator
- 20e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“the hydrogen peroxide (H2O2) and malondialdehyde (MDA) content increased through freeze and cold stress from 20 to 4°C.”
t0“barley (Hordeum vulgare)”
Claim · model-written summary
The increase in hydrogen peroxide and malondialdehyde was greater in sensitive than tolerant barley genotypes.
- Taxon
- barleyHordeum vulgaret0
- Material
- sensitive genotypese0
- Outcome
- hydrogen peroxide (H2O2) and malondialdehyde (MDA) contente1
- Direction
- moree0
- Comparator
- tolerant onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This increase was more in the sensitive genotypes than in the tolerant ones.”
e1“the hydrogen peroxide (H2O2) and malondialdehyde (MDA) content increased”
t0“barley (Hordeum vulgare)”
Claim · model-written summary
Membrane stability index declined after increased lipid peroxidation.
- Taxon
- barleyHordeum vulgaret0
- Compound
- lipid peroxidatione0
- Outcome
- membrane stability index (MSI)e0
- Direction
- decreasede0
- Qualifier
- increase in lipid peroxidatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subsequent to the increase in lipid peroxidation, the membrane stability index (MSI) decreased”
t0“barley (Hordeum vulgare)”
Claim · model-written summary
Electrolyte leakage increased following reduced membrane stability.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- electrolyte leakagee0
- Direction
- increasinge0
- Qualifier
- membrane stability index (MSI) decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the membrane stability index (MSI) decreased, thereby increasing the electrolyte leakage.”
t0“barley (Hordeum vulgare)”
Claim · model-written summary
Sensitive genotypes showed greater reductions in studied parameters.
- Taxon
- barleyHordeum vulgaret0
- Material
- sensitive genotypese0
- Outcome
- studied parameterse0
- Direction
- greater reductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sensitive genotypes demonstrated greater reduction in the studied parameters.”
t0“barley (Hordeum vulgare)”
Claim · model-written summary
CAT and POD activity increased with hydrogen peroxide content.
- Taxon
- barleyHordeum vulgaret0
- Gene
- CAT and PODe0
- Compound
- H2O2e0
- Outcome
- antioxidant enzymes CAT and PODe0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon the increase in the H2O2 content, the antioxidant enzymes CAT and POD also increased”
t0“barley (Hordeum vulgare)”
Claim · model-written summary
Antioxidant enzyme increases were greater in tolerant than sensitive genotypes.
- Taxon
- barleyHordeum vulgaret0
- Material
- tolerant genotypese0
- Outcome
- antioxidant enzymes CAT and PODe1
- Direction
- more increasee0
- Comparator
- sensitive onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with the sensitive ones, the tolerant genotypes indicated more increase in the enzymes.”
e1“the antioxidant enzymes CAT and POD also increased”
t0“barley (Hordeum vulgare)”
Claim · model-written summary
Hydrogen peroxide and malondialdehyde content decreased from −4 to −8°C.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- H2O2 and MDA contente0
- Direction
- decreasede0
- Qualifier
- from −4 to −8°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The H2O2 and MDA content decreased from −4 to −8°C”
t0“barley (Hordeum vulgare)”
Claim · model-written summary
CAT content increased more than POD content during cold stress.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with the POD content, the increase in the CAT content through cold stress was more”
t0“barley (Hordeum vulgare)”
Claim · model-written summary
The authors suggest CAT may be the main inhibitor of H2O2 under cold stress.
- Taxon
- barleyHordeum vulgaret0
- Gene
- CATe0
- Outcome
- main inhibitor of H2O2e0
- Qualifier
- possiblye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was possibly CAT which acted as the main inhibitor of H2O2 in cold stress in the barley plant.”
t0“barley (Hordeum vulgare)”
The same questions in other papers
cold · physiology: oxidative · Hordeum vulgare (barley)Results agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.