Abstract
The influence of light quality and cytokinin content in media on growth, development, photosynthetic pigments and secondary metabolite content of Myrtus communis L. was evaluated in an in vitro culture. Various treatments with light emitting diodes (LEDs): 100% blue (B), a mix of 70% red and 30% blue (RB) and 100% red were applied and compared with a traditional fluorescent lamp as control. Axillary shoots were incubated on Murashige and Skoog medium with 30 g dm −3 sucrose, 0.5% BioAgar, 0.5 μM 1-naphthaleneacetic acid and different concentrations of 6-benzyladenine (BA): 1, 2.5 and 5 µM. Cultures were maintained for 6 weeks in 23/21 ± 1 °C (day/night), 80% relative humidity and 16/8 h photoperiod; photosynthetic photon flux density (PPFD) was 35 µmol m −2 s −1 in all treatments. Light spectra and BA content in media affected biometrical and phytochemical M. communis properties. Red LEDs and 5 µM BA resulted in the highest multiplication rate. The highest shoots were obtained under red LEDs, but with the lowest concentration of cytokinin in media. Fresh weight was greatest on LEDs containing blue light in the spectrum (B and RB); moreover, 5 µM BA increased dry weight. Photosynthetic pigment levels were lower under LED light compared to control lamps. Phenolic acids and flavonoids were identified in M. communis leaf extracts. Myricetin was the major constituent with highest concentration under red LEDs and highest BA level.
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Claims 8
Claim · model-written summary
Red LEDs with 5 µM BA produced the highest multiplication rate.
- Taxon
- Myrtus communis L.
_in_Athens_on_July_4%2C_2023.jpg?width=60)
Myrtus communist0 - Treatment
- Red LEDs and 5 µM BAe0
- Outcome
- multiplication ratee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_in_Athens_on_July_4%2C_2023.jpg?width=500)
Verbatim
e0“Red LEDs and 5 µM BA resulted in the highest multiplication rate.”
t0“Myrtus communis L.”
Claim · model-written summary
Red LEDs with the lowest cytokinin concentration produced the tallest shoots.
- Taxon
- Myrtus communis L.Myrtus communist0
- Treatment
- red LEDse0
- Outcome
- shootse0
- Direction
- higheste0
- Qualifier
- lowest concentration of cytokinin in mediae0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest shoots were obtained under red LEDs, but with the lowest concentration of cytokinin in media.”
t0“Myrtus communis L.”
Claim · model-written summary
Blue-containing LEDs produced the greatest fresh weight.
- Taxon
- Myrtus communis L.Myrtus communist0
- Treatment
- LEDs containing blue light in the spectrum (B and RB)e0
- Outcome
- Fresh weighte0
- Direction
- greateste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fresh weight was greatest on LEDs containing blue light in the spectrum (B and RB)”
t0“Myrtus communis L.”
Claim · model-written summary
5 µM BA increased dry weight.
- Taxon
- Myrtus communis L.Myrtus communist0
- Treatment
- 5 µM BAe0
- Outcome
- dry weighte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5 µM BA increased dry weight.”
t0“Myrtus communis L.”
Claim · model-written summary
LED lighting lowered photosynthetic pigment levels relative to control lamps.
- Taxon
- Myrtus communis L.Myrtus communist0
- Treatment
- LED lighte0
- Outcome
- Photosynthetic pigment levelse0
- Direction
- lowere0
- Comparator
- control lampse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Photosynthetic pigment levels were lower under LED light compared to control lamps.”
t0“Myrtus communis L.”
Claim · model-written summary
Phenolic acids and flavonoids were detected in leaf extracts.
- Taxon
- M. communisMyrtus communise0
- Organ / stage
- leafe0
- Outcome
- Phenolic acids and flavonoidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phenolic acids and flavonoids were identified in M. communis leaf extracts.”
Claim · model-written summary
Myricetin was the major leaf constituent.
- Taxon
- Myrtus communis L.Myrtus communist0
- Outcome
- Myricetine0
- Direction
- majore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Myricetin was the major constituent with highest concentration under red LEDs and highest BA level.”
t0“Myrtus communis L.”
Claim · model-written summary
Myricetin concentration was highest under red LEDs and the highest BA level.
- Taxon
- Myrtus communis L.Myrtus communist0
- Treatment
- red LEDs and highest BA levele0
- Outcome
- Myricetine0
- Direction
- highest concentratione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Myricetin was the major constituent with highest concentration under red LEDs and highest BA level.”
t0“Myrtus communis L.”
The same questions in other papers
N-benzyladenine · pest · Myrtus communisResults agree
1 paper in the same direction as this paper
- positive
N-benzyladenine · growth · Myrtus communisResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.