Abstract
Somatic embryos of olive have been successfully cryopreserved using the droplet-vitrification method on aluminium foil strips (1) after dehydration in PVS2 for 30 min. Although acceptable recovery rates have been obtained (2) and cultures morphology apparently did not change, the influence of this cryopreservation protocol on the somatic embryogenesis process is unknown. The aim of this investigation was to assess the influence of the droplet-vitrification method on the different phases of somatic embryogenesis and to evaluate its applicability to somatic embryos of different genotypes. The results obtained revealed that the genotype plays a key role in the maintenance and maturation phases and determines the regeneration potential of the embryogenic lines. Cryopreservation only affected some specific aspects, such as the proliferation capacity in maintenance medium, germination of newly developed embryos and length of the shoots obtained. Nevertheless, a statistically significant interaction between genotype and cryopreservation was appreciated in different parameters related to cultures proliferation. The influence of cryopreservation on cultures morphology during the maturation phase and embryo germination was also genotype-dependent. Thus, the regeneration potential was not significantly affected by cryopreservation and the quality of the plantlets obtained was similar in both types of cultures, although slightly longer shoots were obtained in plants derived from cryopreserved explants. In relation to the applicability of the droplet-vitrification method to olive somatic embryos of different origin, response to cryopreservation significantly varied depending of the genotype, with recovery rates ranging from 0 to 60%. The appearance of cultures established from frozen somatic embryos was similar to that of the corresponding controls. In conclusion, our investigations show that droplet-vitrification is a reliable procedure for preserving the viability, embryogenic competence and regeneration capacity of olive somatic embryos. Nevertheless, additional optimization of this method is required in order to improve the recovery rates obtained in some of the genotypes tested. (1) Panis B, Piette B & Swennen R (2005) Plant Sci. 168, 45-55. (2) Sánchez-Romero C & Bradaï F (2013) in Abstract Book X Reunión SECIVTV, pp 56.
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Claims 11
Claim · model-written summary
Genotype determined maintenance, maturation and regeneration potential of olive embryogenic lines.
- Taxon
- olive
Olea europaeat0 - Material
- genotypee0
- Outcome
- regeneration potential of the embryogenic linese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results obtained revealed that the genotype plays a key role in the maintenance and maturation phases and determines the regeneration potential of the embryogenic lines.”
t0“olive”
Claim · model-written summary
Cryopreservation affected proliferation capacity, germination of new embryos and shoot length.
- Taxon
- oliveOlea europaeat0
- Treatment
- Cryopreservatione0
- Outcome
- proliferation capacity in maintenance medium, germination of newly developed embryos and length of the shoots obtainede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cryopreservation only affected some specific aspects, such as the proliferation capacity in maintenance medium, germination of newly developed embryos and length of the shoots obtained.”
t0“olive”
Claim · model-written summary
Genotype and cryopreservation interacted significantly for proliferation parameters.
- Taxon
- oliveOlea europaeat0
- Material
- genotypee0
- Treatment
- cryopreservatione0
- Outcome
- different parameters related to cultures proliferatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a statistically significant interaction between genotype and cryopreservation was appreciated in different parameters related to cultures proliferation.”
t0“olive”
Claim · model-written summary
Cryopreservation effects on maturation culture morphology and embryo germination depended on genotype.
- Taxon
- oliveOlea europaeat0
- Material
- genotypee0
- Treatment
- cryopreservatione0
- Outcome
- cultures morphology during the maturation phase and embryo germinatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The influence of cryopreservation on cultures morphology during the maturation phase and embryo germination was also genotype-dependent.”
t0“olive”
Claim · model-written summary
Cryopreservation did not significantly affect regeneration potential.
- Taxon
- oliveOlea europaeat0
- Treatment
- cryopreservatione0
- Outcome
- regeneration potentiale0
- Direction
- not significantly affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the regeneration potential was not significantly affected by cryopreservation”
t0“olive”
Claim · model-written summary
Plantlet quality was similar in cryopreserved and control cultures.
- Taxon
- oliveOlea europaeat0
- Treatment
- cryopreservatione0
- Outcome
- quality of the plantletse0
- Direction
- similare0
- Comparator
- both types of culturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the regeneration potential was not significantly affected by cryopreservation and the quality of the plantlets obtained was similar in both types of cultures”
t0“olive”
Claim · model-written summary
Shoots from cryopreserved olive explants were slightly longer.
- Taxon
- oliveOlea europaeat0
- Treatment
- cryopreserved explantse0
- Outcome
- shootse0
- Direction
- slightly longere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“slightly longer shoots were obtained in plants derived from cryopreserved explants.”
t0“olive”
Claim · model-written summary
Recovery after cryopreservation varied significantly by genotype, from 0 to 60%.
- Taxon
- oliveOlea europaeat0
- Material
- genotypee0
- Treatment
- cryopreservatione0
- Outcome
- recovery ratese0
- Value
- 0 to 60%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“response to cryopreservation significantly varied depending of the genotype, with recovery rates ranging from 0 to 60%.”
t0“olive”
Claim · model-written summary
Cultures from frozen embryos looked similar to their controls.
- Taxon
- oliveOlea europaeat0
- Treatment
- frozen somatic embryose0
- Outcome
- appearance of culturese0
- Direction
- similare0
- Comparator
- corresponding controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The appearance of cultures established from frozen somatic embryos was similar to that of the corresponding controls.”
t0“olive”
Claim · model-written summary
Droplet-vitrification was judged reliable for preserving olive embryo viability, embryogenic competence and regeneration capacity.
- Taxon
- oliveOlea europaeae0
- Outcome
- viability, embryogenic competence and regeneration capacitye0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“droplet-vitrification is a reliable procedure for preserving the viability, embryogenic competence and regeneration capacity of olive somatic embryos.”
t0“olive”
Claim · model-written summary
Additional optimization is recommended to improve recovery for some genotypes.
- Taxon
- oliveOlea europaeat0
- Outcome
- additional optimization of this methode0
- Qualifier
- some of the genotypes testede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“additional optimization of this method is required in order to improve the recovery rates obtained in some of the genotypes tested.”
t0“olive”
The same questions in other papers
in vitro · growth · Olea europaea (common olive)Positive and negative results
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- negative
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See all 19 papers →in vitro · quality: general · Olea europaea (common olive)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.