Abstract
Limiting nitrogen (N) supply contributes to improved resistance to bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) in susceptible rice (Oryza sativa). To understand the regulatory roles of microRNAs (miRNAs) in this phenomenon, 63 differentially expressed overlapping miRNAs in response to Xoo infection and N limitation stress in rice were identified through deep RNA sequencing and stem-loop quantitative real-time PCR. Among these, miR169o was further assessed as a typical overlapping miRNA through the overexpression of the miR169o primary gene. Osa-miR169o-OX plants were taller, and had more biomass accumulation with significantly increased nitrate and total amino acid contents in roots than the wild type (WT). Transcript level assays showed that under different N supply conditions, miR169o oppositely regulated NRT2, and this is reduced under normal N supply conditions but remarkably induced under N-limiting stress. On the other hand, osa-miR169o-OX plants also displayed increased disease lesion lengths and reduced transcriptional levels of defense gene (PR1b, PR10a, PR10b and PAL) compared with the WT after inoculation with Xoo. In addition, miR169o impeded Xoo-mediated NRT transcription. Therefore, the overlapping miR169o contributes to increase N use efficiency and negatively regulates the resistance to BB in rice. Consistently, transient expression of NF-YA genes in rice protoplasts promoted the transcripts of PR genes and NRT2 genes, while it reduced the transcripts of NRT1 genes. Our results provide novel and additional insights into the co ordinated regulatory mechanisms of cross-talk between Xoo infection and N deficiency responses in rice.
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Claims 17
Claim · model-written summary
Nitrogen limitation improved rice resistance to bacterial blight caused by Xoo.
- Taxon
- rice (Oryza sativa)
Oryza sativae0 - Treatment
- Limiting nitrogen (N) supplye0
- Interactor
- Xanthomonas oryzae pv. oryzae (Xoo)
Xanthomonas oryzae pv. oryzaee0pathogen - Outcome
- resistance to bacterial blight (BB)e0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Limiting nitrogen (N) supply contributes to improved resistance to bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) in susceptible rice (Oryza sativa)”
Claim · model-written summary
Sixty-three miRNAs differentially expressed in response to Xoo infection and N limitation were identified in rice.
- Taxon
- riceOryza sativae0
- Treatment
- N limitation stresse0
- Interactor
- Xooe0pathogen
- Outcome
- differentially expressed overlapping miRNAse0
- Value
- 63e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“63 differentially expressed overlapping miRNAs in response to Xoo infection and N limitation stress in rice were identified”
Claim · model-written summary
Osa-miR169o-OX rice plants were taller than wild type plants.
- Taxon
- RiceOryza sativat0
- Material
- Osa-miR169o-OXe0
- Outcome
- tallere0
- Direction
- tallere0
- Comparator
- wild type (WT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Osa-miR169o-OX plants were taller, and had more biomass accumulation with significantly increased nitrate and total amino acid contents in roots than the wild type (WT)”
t0“Rice”
Claim · model-written summary
Osa-miR169o-OX rice plants accumulated more biomass than wild type plants.
- Taxon
- RiceOryza sativat0
- Material
- Osa-miR169o-OXe0
- Outcome
- biomass accumulatione0
- Direction
- moree0
- Comparator
- wild type (WT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Osa-miR169o-OX plants were taller, and had more biomass accumulation with significantly increased nitrate and total amino acid contents in roots than the wild type (WT)”
t0“Rice”
Claim · model-written summary
Osa-miR169o-OX rice roots had significantly increased nitrate content relative to wild type.
- Taxon
- RiceOryza sativat0
- Material
- Osa-miR169o-OXe0
- Organ / stage
- rootse0
- Outcome
- nitratee0
- Direction
- increasede0
- Comparator
- wild type (WT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Osa-miR169o-OX plants were taller, and had more biomass accumulation with significantly increased nitrate and total amino acid contents in roots than the wild type (WT)”
t0“Rice”
Claim · model-written summary
Osa-miR169o-OX rice roots had significantly increased total amino acid content relative to wild type.
- Taxon
- RiceOryza sativat0
- Material
- Osa-miR169o-OXe0
- Organ / stage
- rootse0
- Outcome
- total amino acid contentse0
- Direction
- increasede0
- Comparator
- wild type (WT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Osa-miR169o-OX plants were taller, and had more biomass accumulation with significantly increased nitrate and total amino acid contents in roots than the wild type (WT)”
t0“Rice”
Claim · model-written summary
miR169o reduced NRT2 transcription under normal nitrogen supply.
- Taxon
- RiceOryza sativat0
- Gene
- NRT2e0
- Outcome
- NRT2e0
- Direction
- reducede0
- Qualifier
- under normal N supply conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under different N supply conditions, miR169o oppositely regulated NRT2, and this is reduced under normal N supply conditions”
t0“Rice”
Claim · model-written summary
miR169o induced NRT2 transcription under nitrogen limitation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NRT2, and this is reduced under normal N supply conditions but remarkably induced under N-limiting stress”
t0“Rice”
Claim · model-written summary
After Xoo inoculation, osa-miR169o-OX rice plants had longer disease lesions than wild type.
- Taxon
- RiceOryza sativat0
- Material
- osa-miR169o-OXe0
- Treatment
- inoculation with Xooe0
- Interactor
- Xooe0pathogen
- Outcome
- disease lesion lengthse0
- Direction
- increasede0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“osa-miR169o-OX plants also displayed increased disease lesion lengths and reduced transcriptional levels of defense gene (PR1b, PR10a, PR10b and PAL) compared with the WT after inoculation with Xoo”
t0“Rice”
Claim · model-written summary
After Xoo inoculation, osa-miR169o-OX rice had lower transcription of defense genes PR1b, PR10a and PR10b than wild type.
- Taxon
- RiceOryza sativat0
- Material
- osa-miR169o-OXe0
- Treatment
- inoculation with Xooe0
- Interactor
- Xooe0pathogen
- Gene
- PR1b, PR10a, PR10be0
- Outcome
- transcriptional levels of defense genee0
- Direction
- reducede0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“osa-miR169o-OX plants also displayed increased disease lesion lengths and reduced transcriptional levels of defense gene (PR1b, PR10a, PR10b and PAL) compared with the WT after inoculation with Xoo”
t0“Rice”
Claim · model-written summary
After Xoo inoculation, osa-miR169o-OX rice had lower PAL transcription than wild type.
- Taxon
- RiceOryza sativat0
- Material
- osa-miR169o-OXe0
- Treatment
- inoculation with Xooe0
- Interactor
- Xooe0pathogen
- Gene
- PALe0
- Outcome
- transcriptional levels of defense genee0
- Direction
- reducede0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“osa-miR169o-OX plants also displayed increased disease lesion lengths and reduced transcriptional levels of defense gene (PR1b, PR10a, PR10b and PAL) compared with the WT after inoculation with Xoo”
t0“Rice”
Claim · model-written summary
miR169o impeded Xoo-mediated NRT transcription in rice.
- Taxon
- RiceOryza sativat0
- Interactor
- Xooe0pathogen
- Gene
- miR169oe0
- Outcome
- Xoo-mediated NRT transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR169o impeded Xoo-mediated NRT transcription”
t0“Rice”
Claim · model-written summary
The authors conclude miR169o increases nitrogen use efficiency in rice.
- Taxon
- riceOryza sativae1
- Gene
- miR169oe0
- Outcome
- N use efficiencye0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the overlapping miR169o contributes to increase N use efficiency”
e1“negatively regulates the resistance to BB in rice”
Claim · model-written summary
The authors conclude miR169o negatively regulates rice resistance to bacterial blight.
- Taxon
- riceOryza sativae0
- Gene
- miR169oe1
- Outcome
- resistance to BBe0
- Direction
- negativelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“negatively regulates the resistance to BB in rice”
e1“the overlapping miR169o contributes to increase N use efficiency and negatively regulates the resistance to BB in rice”
Claim · model-written summary
Transient NF-YA expression increased PR gene transcription in rice protoplasts.
- Taxon
- riceOryza sativae0
- Organ / stage
- protoplastse0
- Treatment
- transient expression of NF-YA genese0
- Gene
- PR genese0
- Outcome
- transcripts of PR genese0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient expression of NF-YA genes in rice protoplasts promoted the transcripts of PR genes and NRT2 genes, while it reduced the transcripts of NRT1 genes”
Claim · model-written summary
Transient NF-YA expression increased NRT2 transcription in rice protoplasts.
- Taxon
- riceOryza sativae0
- Organ / stage
- protoplastse0
- Treatment
- transient expression of NF-YA genese0
- Gene
- NRT2 genese0
- Outcome
- transcripts of PR genes and NRT2 genese0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient expression of NF-YA genes in rice protoplasts promoted the transcripts of PR genes and NRT2 genes, while it reduced the transcripts of NRT1 genes”
Claim · model-written summary
Transient NF-YA expression reduced NRT1 transcription in rice protoplasts.
- Taxon
- riceOryza sativae0
- Organ / stage
- protoplastse0
- Treatment
- transient expression of NF-YA genese0
- Gene
- NRT1 genese0
- Outcome
- transcripts of NRT1 genese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient expression of NF-YA genes in rice protoplasts promoted the transcripts of PR genes and NRT2 genes, while it reduced the transcripts of NRT1 genes”
The same questions in other papers
nitrogen · Xanthomonas oryzae pv. oryzae · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
transgenic · molecular: expression · Oryza sativa (rice)Positive and negative results
14 papers in the same direction as this paper · 12 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- negative
- negative
24 papers more in this group
See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.