Abstract
Seed vigour is an imperative trait for the direct seeding of rice. Isopropylmalate synthase (IPMS) catalyses the committed step of leucine (Leu) biosynthesis, but its effect on seed vigour remains unclear. In this study, rice OsIPMS1 and OsIPMS2 was cloned, and the roles of OsIPMS1 in seed vigour were mainly investigated. OsIPMS1 and OsIPMS2 catalyse Leu biosynthesis, and Leu feedback inhibits their IPMS activities. Disruption of OsIPMS1 resulted in low seed vigour under various conditions, which might be tightly associated with the reduction of amino acids in germinating seeds. Eleven amino acids that associated with stress tolerance, GA biosynthesis and tricarboxylic acid (TCA) cycle were significantly reduced in osipms1 mutants compared with those in wide type (WT) during seed germination. Transcriptome analysis indicated that a total of 1209 differentially expressed genes (DEGs) were altered in osipms1a mutant compared with WT at the early germination stage, wherein most of the genes were involved in glycolysis/gluconeogenesis, protein processing, pyruvate, carbon, fructose and mannose metabolism. Further analysis confirmed that the regulation of OsIPMS1 in seed vigour involved in starch hydrolysis, glycolytic activity and energy levels in germinating seeds. The effects of seed priming were tightly associated with the mRNA levels of OsIPMS1 in priming seeds. The OsIPMS1 might be used as a biomarker to determine the best stop time-point of seed priming in rice. This study provides novel insights into the function of OsIPMS1 on seed vigour and should have practical applications in seed priming of rice.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
OsIPMS1 and OsIPMS2 catalyse leucine biosynthesis.
- Taxon
- rice
Oryza sativat0 - Gene
- OsIPMS1 and OsIPMS2e0
- Outcome
- Leu biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsIPMS1 and OsIPMS2 catalyse Leu biosynthesis”
t0“rice”
Claim · model-written summary
Leucine feedback inhibits IPMS activities.
- Taxon
- riceOryza sativat0
- Treatment
- Leu feedbacke0
- Outcome
- IPMS activitiese0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leu feedback inhibits their IPMS activities.”
t0“rice”
Claim · model-written summary
Disrupting OsIPMS1 reduced seed vigour under various conditions.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of OsIPMS1e0
- Gene
- OsIPMS1e0
- Outcome
- seed vigoure0
- Direction
- lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of OsIPMS1 resulted in low seed vigour under various conditions”
t0“rice”
Claim · model-written summary
Eleven amino acids declined significantly in osipms1 mutants during germination.
- Taxon
- riceOryza sativat0
- Material
- osipms1 mutantse0
- Organ / stage
- seed germinatione0
- Treatment
- osipms1 mutantse0
- Outcome
- Eleven amino acidse0
- Direction
- reducede0
- Comparator
- wide type (WT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Eleven amino acids that associated with stress tolerance, GA biosynthesis and tricarboxylic acid (TCA) cycle were significantly reduced in osipms1 mutants compared with those in wide type (WT) during seed germination.”
t0“rice”
Claim · model-written summary
Transcriptome analysis found 1209 differentially expressed genes in osipms1a mutants at early germination.
- Taxon
- riceOryza sativat0
- Material
- osipms1a mutante0
- Organ / stage
- early germination stagee0
- Outcome
- differentially expressed genes (DEGs)e0
- Value
- 1209e0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 1209 differentially expressed genes (DEGs) were altered in osipms1a mutant compared with WT at the early germination stage”
t0“rice”
Claim · model-written summary
Most altered genes were involved in several carbohydrate, protein and energy pathways.
- Taxon
- riceOryza sativat0
- Outcome
- glycolysis/gluconeogenesis, protein processing, pyruvate, carbon, fructose and mannose metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most of the genes were involved in glycolysis/gluconeogenesis, protein processing, pyruvate, carbon, fructose and mannose metabolism.”
t0“rice”
Claim · model-written summary
OsIPMS1 regulation of vigour involved starch hydrolysis, glycolytic activity and energy levels.
- Taxon
- riceOryza sativat0
- Gene
- OsIPMS1e0
- Outcome
- seed vigoure0
- Qualifier
- starch hydrolysis, glycolytic activity and energy levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the regulation of OsIPMS1 in seed vigour involved in starch hydrolysis, glycolytic activity and energy levels in germinating seeds.”
t0“rice”
Claim · model-written summary
Seed priming effects were associated with OsIPMS1 mRNA levels.
- Taxon
- riceOryza sativat0
- Gene
- OsIPMS1e0
- Outcome
- effects of seed priminge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effects of seed priming were tightly associated with the mRNA levels of OsIPMS1 in priming seeds.”
t0“rice”
Claim · model-written summary
OsIPMS1 may serve as a biomarker for choosing when to stop seed priming.
- Taxon
- riceOryza sativae0
- Gene
- OsIPMS1e0
- Outcome
- best stop time-point of seed priminge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OsIPMS1 might be used as a biomarker to determine the best stop time-point of seed priming in rice.”
The same questions in other papers
loss of function · growth · Oryza sativa (rice)Positive and negative results
103 papers in the same direction as this paper · 28 in the opposite direction
- negative
- negative
- negative
128 papers more in this group
See all 132 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.