Abstract
In vitro organogenesis and shoot multiplication protocol of carnation (Dianthus caryophyllus L.) cultivars Madras, Tempo, Dark Rendez Vous and Raggio de Sole was developed using apical and nodal sections of shoots as explants. It has been found that in vitro shoot multiplication is highly variable and genotype dependant. The earliest establishment of shoot sections (8.24 days) with maximum number of shoots explants-1 (2.16) was recorded in MS medium supplemented Benzyl-6-adenine 2 mg l-1. Nodal sections as explants produced more number of shoots as compared to apical portion when inoculated on MS medium supplemented with Benzyl-6-adenine (2 mg l-1). In case of cultivars, earliest shoot establishment (8.97 days) was noticed in cv. Raggio de Sole. On the other hand, number of shoots explant-1 was found to be maximum (2.00) in cv. Dark Rendez Vous. MS medium supplemented with BA 2 mg l-1, GA3 1 mg l-1 and NAA 0.1 mg l-1 was found to be the most effective medium for shoot multiplication, producing 6.74 shoots shoot-1 with longer shoots (3.16 cm). Shoot proliferation was recorded maximum (6.92 shoots shoot-1) in cv. Raggio de Sole, whereas, maximum length of shoots in multiplication medium (3.51 cm) was observed in cv. Tempo. The regenerated shoots were rooted on MS medium added with NAA 2 mg l-1. The rooted plantlets were hardened in medium containing cocopeat and sand (1:1, v/v) and successfully transferred to field conditions.
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Claims 12
Claim · model-written summary
In-vitro shoot multiplication varied by genotype.
- Taxon
- carnation
Dianthus caryophyllust0 - Material
- genotypee0
- Outcome
- in vitro shoot multiplicatione0
- Direction
- highly variablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in vitro shoot multiplication is highly variable and genotype dependant”
t0“Carnation”
Claim · model-written summary
MS medium with Benzyl-6-adenine at 2 mg l-1 gave the earliest shoot-section establishment, at 8.24 days.
- Taxon
- carnationDianthus caryophyllust0
- Treatment
- MS medium supplemented Benzyl-6-adenine 2 mg l-1e0
- Outcome
- establishment of shoot sectionse0
- Direction
- earlieste0
- Value
- 8.24 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The earliest establishment of shoot sections (8.24 days) with maximum number of shoots explants-1 (2.16) was recorded in MS medium supplemented Benzyl-6-adenine 2 mg l-1.”
t0“Carnation”
Claim · model-written summary
The same medium produced the maximum number of shoots per explant, 2.16.
- Taxon
- carnationDianthus caryophyllust0
- Treatment
- MS medium supplemented Benzyl-6-adenine 2 mg l-1e0
- Outcome
- number of shoots explants-1e0
- Direction
- maximume0
- Value
- 2.16e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The earliest establishment of shoot sections (8.24 days) with maximum number of shoots explants-1 (2.16) was recorded in MS medium supplemented Benzyl-6-adenine 2 mg l-1.”
t0“Carnation”
Claim · model-written summary
Nodal sections produced more shoots than apical sections on the specified BA medium.
- Taxon
- carnationDianthus caryophyllust0
- Treatment
- Nodal sectionse0
- Outcome
- number of shootse0
- Direction
- moree0
- Qualifier
- MS medium supplemented with Benzyl-6-adenine (2 mg l-1)e0
- Comparator
- apical portione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nodal sections as explants produced more number of shoots as compared to apical portion when inoculated on MS medium supplemented with Benzyl-6-adenine (2 mg l-1).”
t0“Carnation”
Claim · model-written summary
Raggio de Sole established shoots earliest among cultivars, at 8.97 days.
- Taxon
- carnationDianthus caryophyllust0
- Material
- Raggio de Solee0
- Outcome
- shoot establishmente0
- Direction
- earlieste0
- Value
- 8.97 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“earliest shoot establishment (8.97 days) was noticed in cv. Raggio de Sole”
t0“Carnation”
Claim · model-written summary
Dark Rendez Vous had the maximum shoots per explant, 2.00.
- Taxon
- carnationDianthus caryophyllust0
- Material
- Dark Rendez Vouse0
- Outcome
- number of shoots explant-1e0
- Direction
- maximume0
- Value
- 2.00e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“number of shoots explant-1 was found to be maximum (2.00) in cv. Dark Rendez Vous”
t0“Carnation”
Claim · model-written summary
The BA, GA3 and NAA medium produced 6.74 shoots per shoot and was most effective for multiplication.
- Taxon
- carnationDianthus caryophyllust0
- Treatment
- MS medium supplemented with BA 2 mg l-1, GA3 1 mg l-1 and NAA 0.1 mg l-1e0
- Outcome
- shoot multiplicatione0
- Direction
- most effectivee0
- Value
- 6.74 shoots shoot-1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with BA 2 mg l-1, GA3 1 mg l-1 and NAA 0.1 mg l-1 was found to be the most effective medium for shoot multiplication, producing 6.74 shoots shoot-1 with longer shoots (3.16 cm).”
t0“Carnation”
Claim · model-written summary
The BA, GA3 and NAA medium produced longer shoots of 3.16 cm.
- Taxon
- carnationDianthus caryophyllust0
- Treatment
- MS medium supplemented with BA 2 mg l-1, GA3 1 mg l-1 and NAA 0.1 mg l-1e0
- Outcome
- shootse0
- Direction
- longere0
- Value
- 3.16 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with BA 2 mg l-1, GA3 1 mg l-1 and NAA 0.1 mg l-1 was found to be the most effective medium for shoot multiplication, producing 6.74 shoots shoot-1 with longer shoots (3.16 cm).”
t0“Carnation”
Claim · model-written summary
Raggio de Sole had the maximum shoot proliferation at 6.92 shoots per shoot.
- Taxon
- carnationDianthus caryophyllust0
- Material
- Raggio de Solee0
- Outcome
- Shoot proliferatione0
- Direction
- maximume0
- Value
- 6.92 shoots shoot-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot proliferation was recorded maximum (6.92 shoots shoot-1) in cv. Raggio de Sole”
t0“Carnation”
Claim · model-written summary
Tempo had maximum shoot length in multiplication medium, at 3.51 cm.
- Taxon
- carnationDianthus caryophyllust0
- Material
- Tempoe0
- Outcome
- length of shootse0
- Direction
- maximume0
- Value
- 3.51 cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum length of shoots in multiplication medium (3.51 cm) was observed in cv. Tempo”
t0“Carnation”
Claim · model-written summary
Regenerated shoots rooted on MS medium with NAA at 2 mg l-1.
- Taxon
- carnationDianthus caryophyllust0
- Treatment
- MS medium added with NAA 2 mg l-1e0
- Outcome
- regenerated shoots were rootede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regenerated shoots were rooted on MS medium added with NAA 2 mg l-1.”
t0“Carnation”
Claim · model-written summary
Hardened plantlets transferred successfully to field conditions.
- Taxon
- carnationDianthus caryophyllust0
- Treatment
- medium containing cocopeat and sand (1:1, v/v)e0
- Outcome
- successfully transferred to field conditionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rooted plantlets were hardened in medium containing cocopeat and sand (1:1, v/v) and successfully transferred to field conditions.”
t0“Carnation”
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