Abstract
Carnation (Dianthus caryophyllus L.) ‘Purple Beauty’ at micropropagation stage 3 was cultured under two levels each of medium sucrose concentration (0 and 30 g·L-1), photosynthetic photon flux density (50 and 200 μmol·m-2·s-1 PPFD), and CO2 concentration (350 and 1,000 μmol·mol-1), in a factorial experimental design, consisting of all eight possible treatment combinations. Shoot tip explants, obtained from in vitro-grown plantlets, were cultured on 50 mL agar-solidified Murashige and Skoog medium per container. Each culture container was sealed with a rigid lid vented by a high-efficiency particulate air filter attached to a 10 mm diameter hole made in the lid center, with an estimated number of 2.8 air exchanges per hour. All cultures were maintained for four weeks at 24°C/22°C (day/night) temperatures, 70%–80% relative humidity, and a 16 h/8 h (day/night) photoperiod was provided by white light-emitting diodes. The treatment combining high light intensity (200 μmol·m-2·s-1), high CO2 concentration (1,000 μmol·mol-1), and without supplementation of sucrose to the medium (i.e., photo-autotrophic conditions) resulted in better plantlet growth and development, with higher values being observed in terms of total fresh weight, tissue water content, leaf length, leaf width, and total chlorophyll content than in plantlets grown under the other treatments.
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Claims 2
Claim · model-written summary
High light and CO2 without sucrose increased carnation plantlet fresh weight, tissue water content and leaf length relative to other treatments.
- Taxon
- Carnation
Dianthus caryophylluse0 - Material
- ‘Purple Beauty’e0
- Treatment
- high light intensity (200 μmol·m-2·s-1), high CO2 concentration (1,000 μmol·mol-1), and without supplementation of sucrosee1
- Outcome
- total fresh weight, tissue water content, leaf lengthe1
- Direction
- highere1
- Comparator
- plantlets grown under the other treatmentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Carnation (Dianthus caryophyllus L.) ‘Purple Beauty’ at micropropagation stage 3”
e1“The treatment combining high light intensity (200 μmol·m-2·s-1), high CO2 concentration (1,000 μmol·mol-1), and without supplementation of sucrose to the medium (i.e., photo-autotrophic conditions) resulted in better plantlet growth and development, with higher values being observed in terms of total fresh weight, tissue water content, leaf length, leaf width, and total chlorophyll content than in plantlets grown under the other treatments.”
Claim · model-written summary
The same treatment increased carnation plantlet leaf width and total chlorophyll content relative to other treatments.
- Taxon
- CarnationDianthus caryophylluse0
- Material
- ‘Purple Beauty’e0
- Treatment
- high light intensity (200 μmol·m-2·s-1), high CO2 concentration (1,000 μmol·mol-1), and without supplementation of sucrosee1
- Outcome
- leaf width, and total chlorophyll contente1
- Direction
- highere1
- Comparator
- plantlets grown under the other treatmentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Carnation (Dianthus caryophyllus L.) ‘Purple Beauty’ at micropropagation stage 3”
e1“The treatment combining high light intensity (200 μmol·m-2·s-1), high CO2 concentration (1,000 μmol·mol-1), and without supplementation of sucrose to the medium (i.e., photo-autotrophic conditions) resulted in better plantlet growth and development, with higher values being observed in terms of total fresh weight, tissue water content, leaf length, leaf width, and total chlorophyll content than in plantlets grown under the other treatments.”
The same questions in other papers
sucrose · growth · Dianthus caryophyllus (carnation)Positive and negative results
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- negative
- positive
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light · physiology: pigments · Dianthus caryophyllus (carnation)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
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light · growth · Dianthus caryophyllus (carnation)Positive and negative results
8 papers in the same direction as this paper · 5 in the opposite direction
- positive
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- no effect
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See all 14 papers →co2 · growth · Dianthus caryophyllus (carnation)Results agree
2 papers in the same direction as this paper
- positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.