Abstract
Dormancy is one of the least understood phenomena in plant biology; however, bud/corm dormancy is an important economic trait in agricultural/horticultural breeding. In this study, we isolated an ABA biosynthesis gene, GhNCED, from the transcriptome database of corm dormancy release (CDR), and characterized its negative role in regulating CDR. To understand transcriptional regulation of GhNCED, yeast one-hybrid screening was conducted and GhTCP19 was identified and shown to regulate GhNCED expression directly. An in planta assay showed that GhTCP19 negatively regulates GhNCED expression. GhTCP19 is dramatically induced by exogenous cytokinins (CKs) and is induced during CDR. Silencing of GhTCP19 in dormant cormels delayed CDR, resulting in higher expression of GhNCED and ABA levels. Meanwhile, endogenous CK biosynthesis and signaling were inhibited in GhTCP19-silenced cormels. Taken together, our results reveal that GhTCP19 is a positive regulator of the CDR process by repressing expression of an ABA biosynthesis gene (GhNCED), promoting CK biosynthesis (GhIPT) and signal transduction (GhARR) as well as inducing cyclin genes. This study expands our knowledge on CDR which is mediated by TCP family members.
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Claims 12
Claim · model-written summary
GhNCED negatively regulated corm dormancy release.
GladiolusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhNCED, from the transcriptome database of corm dormancy release (CDR), and characterized its negative role in regulating CDR”
t0“Gladiolus”
Claim · model-written summary
GhTCP19 directly regulated GhNCED expression.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhTCP19 was identified and shown to regulate GhNCED expression directly”
t0“Gladiolus”
Claim · model-written summary
GhTCP19 negatively regulated GhNCED expression in planta.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhTCP19 negatively regulates GhNCED expression”
t0“Gladiolus”
Claim · model-written summary
Exogenous cytokinins dramatically induced GhTCP19.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhTCP19 is dramatically induced by exogenous cytokinins (CKs)”
t0“Gladiolus”
Claim · model-written summary
GhTCP19 was induced during corm dormancy release.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is induced during CDR”
e1“GhTCP19 is dramatically induced”
t0“Gladiolus”
Claim · model-written summary
GhTCP19 silencing delayed corm dormancy release.
- Organ / stage
- dormant cormelse0
- Treatment
- Silencing of GhTCP19e0
- Gene
- GhTCP19e0
- Outcome
- CDRe0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of GhTCP19 in dormant cormels delayed CDR”
t0“Gladiolus”
Claim · model-written summary
GhTCP19 silencing increased GhNCED expression and ABA levels.
- Treatment
- Silencing of GhTCP19e0
- Gene
- GhNCEDe0
- Outcome
- expression of GhNCED and ABA levelse0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of GhTCP19 in dormant cormels delayed CDR, resulting in higher expression of GhNCED and ABA levels”
t0“Gladiolus”
Claim · model-written summary
GhTCP19 silencing inhibited endogenous cytokinin biosynthesis and signaling.
- Treatment
- GhTCP19-silenced cormelse0
- Outcome
- endogenous CK biosynthesis and signalinge0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endogenous CK biosynthesis and signaling were inhibited in GhTCP19-silenced cormels”
t0“Gladiolus”
Claim · model-written summary
GhTCP19 promoted corm dormancy release by repressing GhNCED.
- Gene
- GhTCP19e0
- Outcome
- CDR processe0
- Qualifier
- repressing expression of an ABA biosynthesis gene (GhNCED)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhTCP19 is a positive regulator of the CDR process by repressing expression of an ABA biosynthesis gene (GhNCED)”
t0“Gladiolus”
Claim · model-written summary
GhTCP19 promoted cytokinin biosynthesis through GhIPT.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoting CK biosynthesis (GhIPT)”
e1“GhTCP19 is a positive regulator”
t0“Gladiolus”
Claim · model-written summary
GhTCP19 promoted cytokinin signal transduction through GhARR.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“signal transduction (GhARR)”
e1“GhTCP19 is a positive regulator”
t0“Gladiolus”
Claim · model-written summary
GhTCP19 induced cyclin genes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inducing cyclin genes”
e1“GhTCP19 is a positive regulator”
t0“Gladiolus”
The same questions in other papers
loss of function · growth · GladiolusResults agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.