Abstract
Gladiolus hybridus is one of the most popular flowers worldwide. However, its corm dormancy characteristic largely limits its off-season production. Long-term cold treatment (LT), which increases sugar content and reduces abscisic acid (ABA), is an efficient approach to accelerate corm dormancy release (CDR). Here, we identified a GhbZIP30-GhCCCH17 module that mediates the antagonism between sugars and ABA during CDR. We showed that sugars promoted CDR by reducing ABA levels in Gladiolus. Our data demonstrated that GhbZIP30 transcription factor directly binds the GhCCCH17 zinc finger promoter and activates its transcription, confirmed by yeast one-hybrid, dual-luciferase (Dual-LUC), chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) and electrophoretic mobility shift assay (EMSA). GhCCCH17 is a transcriptional activator, and its nuclear localisation is altered by surcose and cytokinin treatments. Both GhbZIP30 and GhCCCH17 positively respond to LT, sugars, and cytokinin treatments. Silencing GhbZIP30 or GhCCCH17 resulted in delayed CDR by regulating ABA metabolic genes, while their overexpression promoted CDR. Taken together, we propose that the GhbZIP30-GhCCCH17 module is involved in cold- and glucose-induced CDR by regulating ABA metabolic genes.
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Claims 10
Claim · model-written summary
Long-term cold treatment increases sugar and reduces ABA while accelerating corm dormancy release.
GladiolusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Long-term cold treatment (LT), which increases sugar content and reduces abscisic acid (ABA), is an efficient approach to accelerate corm dormancy release (CDR)”
t0“Gladiolus”
Claim · model-written summary
Sugars promoted dormancy release by reducing ABA levels.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sugars promoted CDR by reducing ABA levels in Gladiolus”
Claim · model-written summary
GhbZIP30 directly binds the GhCCCH17 promoter and activates transcription.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhbZIP30 transcription factor directly binds the GhCCCH17 zinc finger promoter and activates its transcription”
t0“Gladiolus”
Claim · model-written summary
GhCCCH17 is a transcriptional activator.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhCCCH17 is a transcriptional activator”
t0“Gladiolus”
Claim · model-written summary
Sucrose and cytokinin treatments alter GhCCCH17 nuclear localisation.
Not reportedGeneComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its nuclear localisation is altered by surcose and cytokinin treatments”
t0“Gladiolus”
Claim · model-written summary
GhbZIP30 and GhCCCH17 respond positively to cold, sugars and cytokinin.
- Treatment
- LT, sugars, and cytokinin treatmentse0
- Gene
- GhbZIP30 and GhCCCH17e0
- Outcome
- positively responde0
- Direction
- positivelye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both GhbZIP30 and GhCCCH17 positively respond to LT, sugars, and cytokinin treatments”
t0“Gladiolus”
Claim · model-written summary
Silencing GhbZIP30 delayed corm dormancy release.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing GhbZIP30 or GhCCCH17 resulted in delayed CDR by regulating ABA metabolic genes”
t0“Gladiolus”
Claim · model-written summary
Silencing GhCCCH17 delayed corm dormancy release.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing GhbZIP30 or GhCCCH17 resulted in delayed CDR by regulating ABA metabolic genes”
t0“Gladiolus”
Claim · model-written summary
Overexpression of GhbZIP30 promoted corm dormancy release.
Not reportedTaxonComparator
Verbatim
e0“their overexpression promoted CDR”
t0“GhbZIP30‐GhCCCH17 module”
Claim · model-written summary
Overexpression of GhCCCH17 promoted corm dormancy release.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their overexpression promoted CDR”
t0“Gladiolus”
The same questions in other papers
loss of function · growth · GladiolusResults agree
1 paper in the same direction as this paper
- negative
cold · growth · GladiolusResults agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.