Paper · 2018 · Egyptian Journal of Botany

Effect Of Sodium Nitro-Prusside( Snp) Pretreatment on Ammonia Assimilating Enzymes of Salt Stressed Tomato Leaves (Lycopersicon esculentum)

Hala Aly; Nabil Saber; Amany Mohamed

12
claims
25
verbatim quotes
1
citations
1
research areas
✓
Open access

Abstract

THE EFFECTS of treatment with 100mM NaCl on photosynthetic activity, protein and proline contents , activities of key nitrogen metabolism enzymes, nitrate reductase (NR) and nitrite reductase (NiR), ammonia assimilating enzymes: Glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (NADH-GDH) activities and finally ammonia and nitrate contents were investigated in 40-day old leaves of tomato plants. There was a decline in total protein (TP) and insoluble protein (InsP) fractions accompanied with a significant increase in proline and soluble protein contents in response to NaCl – stress. Under salinized conditions, there was a significant inhibition in all tested leaf gas exchange parameters, stomatal conductance (gs), internal CO2 concentration (Ci) and hence, CO2 assimilation (A). Also, a significant inhibition of NR & NiR enzymes and a strongly decrease in nitrate content were observed. In contrast, ammonia assimilating enzymes (GS, GOGAT, NADH-GDH and NAD-GDH) activities were obviously increased in NaCl – salinized tomato leaves, this accompanied with a significant increase in ammonia content. Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8h were elevated-to some extent- all the studied parameters and there was an improvement in TP, proline, all gas exchange parameters, NR & NiR activities and nitrate content. While ammonia assimilating enzymes (GS, GOGAT, NADH-GDH and NAD-GDH) activities and ammonia content were significantly decreased compared to NaCl - salinized tomato leaves.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 12

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

NaCl reduced total and insoluble protein in tomato leaves.

Treatment
NaCl – stresse0
Outcome
total protein (TP) and insoluble protein (InsP) fractionse0
Direction
declinee0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“There was a decline in total protein (TP) and insoluble protein (InsP) fractions accompanied with a significant increase in proline and soluble protein contents in response to NaCl – stress.”

✓ located verbatim in the source · abstract, characters 431–621 · show in text ↑
t0

“tomato”

✓ located after whitespace/quote normalisation · title, characters 100–106 · show in text ↑
Intervention effectobservedoriginal resultmixedsignificant (as stated)measured

Claim · model-written summary

NaCl significantly increased proline and soluble protein contents in tomato leaves.

Treatment
NaCl – stresse0
Outcome
proline and soluble protein contentse0
Direction
increasee0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“There was a decline in total protein (TP) and insoluble protein (InsP) fractions accompanied with a significant increase in proline and soluble protein contents in response to NaCl – stress.”

✓ located verbatim in the source · abstract, characters 431–621 · show in text ↑
t0

“tomato”

✓ located after whitespace/quote normalisation · title, characters 100–106 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

NaCl significantly inhibited tomato leaf stomatal conductance, internal CO2 concentration and CO2 assimilation.

Treatment
salinized conditionse0
Outcome
stomatal conductance (gs), internal CO2 concentration (Ci) and hence, CO2 assimilation (A)e0
Direction
inhibitione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Under salinized conditions, there was a significant inhibition in all tested leaf gas exchange parameters, stomatal conductance (gs), internal CO2 concentration (Ci) and hence, CO2 assimilation (A).”

✓ located verbatim in the source · abstract, characters 622–820 · show in text ↑
t0

“tomato”

✓ located after whitespace/quote normalisation · title, characters 100–106 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

NaCl significantly inhibited tomato leaf nitrate reductase and nitrite reductase activity.

Treatment
NaCl – stresse1
Outcome
NR & NiR enzymese0
Direction
inhibitione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Also, a significant inhibition of NR & NiR enzymes and a strongly decrease in nitrate content were observed.”

✓ located verbatim in the source · abstract, characters 821–929 · show in text ↑
t0

“tomato”

✓ located after whitespace/quote normalisation · title, characters 100–106 · show in text ↑
e1

“in response to NaCl – stress.”

✓ located verbatim in the source · abstract, characters 592–621 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

NaCl strongly decreased nitrate content in tomato leaves.

Treatment
NaCl – stresse1
Outcome
nitrate contente0
Direction
decreasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a strongly decrease in nitrate content were observed.”

✓ located verbatim in the source · abstract, characters 876–929 · show in text ↑
t0

“tomato”

✓ located after whitespace/quote normalisation · title, characters 100–106 · show in text ↑
e1

“in response to NaCl – stress.”

✓ located verbatim in the source · abstract, characters 592–621 · show in text ↑
Intervention effectobservedoriginal resultmixedmeasured

Claim · model-written summary

NaCl increased GS, GOGAT, NADH-GDH and NAD-GDH activities in tomato leaves.

Treatment
NaCl – salinizede0
Outcome
ammonia assimilating enzymes (GS, GOGAT, NADH-GDH and NAD-GDH) activitiese0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“ammonia assimilating enzymes (GS, GOGAT, NADH-GDH and NAD-GDH) activities were obviously increased in NaCl – salinized tomato leaves”

✓ located verbatim in the source · abstract, characters 943–1075 · show in text ↑
Intervention effectobservedoriginal resultmixedsignificant (as stated)measured

Claim · model-written summary

NaCl significantly increased ammonia content in tomato leaves.

Treatment
NaCl – salinizede1
Outcome
ammonia contente0
Direction
increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“this accompanied with a significant increase in ammonia content.”

✓ located verbatim in the source · abstract, characters 1077–1141 · show in text ↑
e1

“NaCl – salinized tomato leaves”

✓ located verbatim in the source · abstract, characters 1045–1075 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sodium nitroprusside seed soaking improved total protein and proline in salt-stressed tomato leaves.

Treatment
Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8he0
Outcome
TP, prolinee0
Direction
improvemente0
Comparator
NaCl - salinized tomato leavese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8h were elevated-to some extent- all the studied parameters and there was an improvement in TP, proline, all gas exchange parameters, NR & NiR activities and nitrate content.”

✓ located verbatim in the source · abstract, characters 1142–1376 · show in text ↑
e1

“compared to NaCl - salinized tomato leaves.”

✓ located verbatim in the source · abstract, characters 1506–1549 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sodium nitroprusside seed soaking improved gas exchange parameters in salt-stressed tomato leaves.

Treatment
Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8he0
Outcome
all gas exchange parameterse0
Direction
improvemente0
Comparator
NaCl - salinized tomato leavese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8h were elevated-to some extent- all the studied parameters and there was an improvement in TP, proline, all gas exchange parameters, NR & NiR activities and nitrate content.”

✓ located verbatim in the source · abstract, characters 1142–1376 · show in text ↑
e1

“compared to NaCl - salinized tomato leaves.”

✓ located verbatim in the source · abstract, characters 1506–1549 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sodium nitroprusside seed soaking improved NR and NiR activities and nitrate content in salt-stressed tomato leaves.

Treatment
Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8he0
Outcome
NR & NiR activities and nitrate contente0
Direction
improvemente0
Comparator
NaCl - salinized tomato leavese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8h were elevated-to some extent- all the studied parameters and there was an improvement in TP, proline, all gas exchange parameters, NR & NiR activities and nitrate content.”

✓ located verbatim in the source · abstract, characters 1142–1376 · show in text ↑
e1

“compared to NaCl - salinized tomato leaves.”

✓ located verbatim in the source · abstract, characters 1506–1549 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Sodium nitroprusside seed soaking significantly decreased ammonia-assimilating enzyme activities relative to salinized tomato leaves.

Treatment
Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8he1
Outcome
ammonia assimilating enzymes (GS, GOGAT, NADH-GDH and NAD-GDH) activitiese0
Direction
decreasede0
Comparator
NaCl - salinized tomato leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“While ammonia assimilating enzymes (GS, GOGAT, NADH-GDH and NAD-GDH) activities and ammonia content were significantly decreased compared to NaCl - salinized tomato leaves.”

✓ located verbatim in the source · abstract, characters 1377–1549 · show in text ↑
e1

“Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8h”

✓ located verbatim in the source · abstract, characters 1142–1204 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Sodium nitroprusside seed soaking significantly decreased ammonia content relative to salinized tomato leaves.

Treatment
Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8he1
Outcome
ammonia contente0
Direction
decreasede0
Comparator
NaCl - salinized tomato leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“While ammonia assimilating enzymes (GS, GOGAT, NADH-GDH and NAD-GDH) activities and ammonia content were significantly decreased compared to NaCl - salinized tomato leaves.”

✓ located verbatim in the source · abstract, characters 1377–1549 · show in text ↑
e1

“Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8h”

✓ located verbatim in the source · abstract, characters 1142–1204 · show in text ↑

sodium nitroprusside · physiology: enzyme activity · Solanum lycopersicum (tomato)Positive and negative results

6 papers: 4 positive, 2 negative or no effect

3 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. no effect
  3. positive

2 papers more in this group

See all 6 papers →

sodium chloride · quality: protein · Solanum lycopersicum (tomato)Positive and negative results

6 papers: 1 positive, 5 negative or no effect

4 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

2 papers more in this group

See all 6 papers →

sodium nitroprusside · molecular: metabolites · Solanum lycopersicum (tomato)Positive and negative results

15 papers: 13 positive, 2 negative or no effect

12 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. no effect
  3. positive

11 papers more in this group

See all 15 papers →

sodium chloride · nutrient: content · Solanum lycopersicum (tomato)Positive and negative results

18 papers: 2 positive, 16 negative or no effect

15 papers in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. positive
  3. negative

14 papers more in this group

See all 18 papers →

sodium chloride · physiology: enzyme activity · Solanum lycopersicum (tomato)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. no effect
See all 2 papers →

sodium nitroprusside · physiology: gas exchange · Solanum lycopersicum (tomato)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

salinity · physiology: photosynthesis · Solanum lycopersicum (tomato)Results differ

69 papers: 9 positive, 60 negative or no effect

59 papers in the same direction as this paper · 9 in the opposite direction

  1. positive
  2. positive
  3. positive

65 papers more in this group

See all 69 papers →

application · quality: protein · Solanum lycopersicum (tomato)Positive and negative results

10 papers: 9 positive, 1 negative or no effect

8 papers in the same direction as this paper · 1 reporting no difference from an alternative

  1. no effect
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

8 of 11 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.