Abstract
ABSTRACT NAD kinase is thought to play an important role in the plant cellular responses to biotic and abiotic stress as one of the isoforms of the enzyme is activated by the Ca 2 + –calmodulin (CaM) complex. NAD kinase activity was measured after short‐term NaCl stress applied to isolated cells from Lycopersicon esculentum , var. Volgogradskij (NaCl‐sensitive tomato) and L. pimpinellifolium , acc. PE2 (NaCl‐tolerant species). NAD kinase activity remained constant in the sensitive species, whereas a sharp decrease was observed in the tolerant one. After salt treatment, an induction of the calmodulin gene(s) was observed in the two species, together with a 30–50% decrease in ‘active’ CaM content, i.e. CaM able to activate purified NAD kinase, in L. pimpinellifolium . The decrease in NAD kinase activity could not, however, be fully explained by this decrease in active CaM content. A similar decrease in NAD kinase activity was also recorded with other ionic stresses and exposure to high temperatures, but not in the case of drought, exposure to low temperatures, hormonal (indole‐3‐acetic acid and abscisic acid) or H 2 O 2 treatments. External Ca 2 + certainly plays a role in the biochemical mechanism(s) leading to NAD kinase inhibition, while no role could be shown for intracellular Ca 2 + . In addition, after salt stress, a modification of the redox state of NAD kinase seems to be responsible for the inhibition of the enzyme.
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Claims 9
Claim · model-written summary
In the tolerant L. pimpinellifolium, NAD kinase activity sharply decreased after short-term NaCl stress, whereas it remained constant in the sensitive species.
- Taxon
- L. pimpinellifolium
Solanum pimpinellifoliume0 - Treatment
- short‐term NaCl stresse0
- Outcome
- NAD kinase activitye1
- Direction
- sharp decreasee1
- Comparator
- sensitive speciese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAD kinase activity was measured after short‐term NaCl stress applied to isolated cells from Lycopersicon esculentum , var. Volgogradskij (NaCl‐sensitive tomato) and L. pimpinellifolium , acc. PE2 (NaCl‐tolerant species)”
e1“NAD kinase activity remained constant in the sensitive species, whereas a sharp decrease was observed in the tolerant one”
Claim · model-written summary
NAD kinase activity in Lycopersicon esculentum var. Volgogradskij remained constant after short-term NaCl stress.
- Taxon
- Lycopersicon esculentum
Solanum lycopersicume0 - Material
- Volgogradskije0
- Treatment
- short‐term NaCl stresse0
- Outcome
- NAD kinase activitye1
- Direction
- remained constante1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAD kinase activity was measured after short‐term NaCl stress applied to isolated cells from Lycopersicon esculentum , var. Volgogradskij (NaCl‐sensitive tomato) and L. pimpinellifolium , acc. PE2 (NaCl‐tolerant species)”
e1“NAD kinase activity remained constant in the sensitive species, whereas a sharp decrease was observed in the tolerant one”
Claim · model-written summary
After salt treatment, calmodulin gene(s) were induced in both tomato species.
- Taxon
- Lycopersicon esculentumSolanum lycopersicume0
- Treatment
- salt treatmente2
- Gene
- calmodulin gene(s)e2
- Outcome
- induction of the calmodulin gene(s)e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAD kinase activity was measured after short‐term NaCl stress applied to isolated cells from Lycopersicon esculentum , var. Volgogradskij (NaCl‐sensitive tomato) and L. pimpinellifolium , acc. PE2 (NaCl‐tolerant species)”
e2“After salt treatment, an induction of the calmodulin gene(s) was observed in the two species”
Claim · model-written summary
After salt treatment, active CaM content decreased by 30-50% in L. pimpinellifolium.
- Taxon
- L. pimpinellifoliumSolanum pimpinellifoliume3
- Treatment
- salt treatmente2
- Outcome
- CaM contente3
- Direction
- decreasee3
- Value
- 30–50%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“After salt treatment, an induction of the calmodulin gene(s) was observed in the two species”
e3“together with a 30–50% decrease in ‘active’ CaM content, i.e. CaM able to activate purified NAD kinase, in L. pimpinellifolium”
Claim · model-written summary
Other ionic stresses and high temperatures decreased NAD kinase activity in L. pimpinellifolium.
- Taxon
- L. pimpinellifoliumSolanum pimpinellifoliume0
- Treatment
- other ionic stresses and exposure to high temperaturese4
- Outcome
- NAD kinase activitye4
- Direction
- decreasee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAD kinase activity was measured after short‐term NaCl stress applied to isolated cells from Lycopersicon esculentum , var. Volgogradskij (NaCl‐sensitive tomato) and L. pimpinellifolium , acc. PE2 (NaCl‐tolerant species)”
e4“A similar decrease in NAD kinase activity was also recorded with other ionic stresses and exposure to high temperatures, but not in the case of drought, exposure to low temperatures”
Claim · model-written summary
Drought and low temperature exposure did not decrease NAD kinase activity in L. pimpinellifolium.
- Taxon
- L. pimpinellifoliumSolanum pimpinellifoliume0
- Treatment
- drought, exposure to low temperaturese4
- Outcome
- NAD kinase activitye4
- Direction
- no_changeinferrede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAD kinase activity was measured after short‐term NaCl stress applied to isolated cells from Lycopersicon esculentum , var. Volgogradskij (NaCl‐sensitive tomato) and L. pimpinellifolium , acc. PE2 (NaCl‐tolerant species)”
e4“A similar decrease in NAD kinase activity was also recorded with other ionic stresses and exposure to high temperatures, but not in the case of drought, exposure to low temperatures”
Claim · model-written summary
External Ca2+ appears to play a role in the mechanism leading to NAD kinase inhibition.
- Taxon
- L. pimpinellifoliumSolanum pimpinellifoliume0
- Compound
- External Ca 2 +e6
- Outcome
- NAD kinase inhibitione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAD kinase activity was measured after short‐term NaCl stress applied to isolated cells from Lycopersicon esculentum , var. Volgogradskij (NaCl‐sensitive tomato) and L. pimpinellifolium , acc. PE2 (NaCl‐tolerant species)”
e6“External Ca 2 + certainly plays a role in the biochemical mechanism(s) leading to NAD kinase inhibition, while no role could be shown for intracellular Ca 2 +”
Claim · model-written summary
No role could be shown for intracellular Ca2+ in NAD kinase inhibition.
- Taxon
- L. pimpinellifoliumSolanum pimpinellifoliume0
- Compound
- intracellular Ca 2 +e6
- Outcome
- no role could be shown for intracellular Ca 2 +e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAD kinase activity was measured after short‐term NaCl stress applied to isolated cells from Lycopersicon esculentum , var. Volgogradskij (NaCl‐sensitive tomato) and L. pimpinellifolium , acc. PE2 (NaCl‐tolerant species)”
e6“External Ca 2 + certainly plays a role in the biochemical mechanism(s) leading to NAD kinase inhibition, while no role could be shown for intracellular Ca 2 +”
Claim · model-written summary
A modification of the redox state of NAD kinase after salt stress seems responsible for the enzyme inhibition.
- Taxon
- L. pimpinellifoliumSolanum pimpinellifoliume0
- Outcome
- inhibition of the enzymee7
- Qualifier
- modification of the redox state of NAD kinasee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAD kinase activity was measured after short‐term NaCl stress applied to isolated cells from Lycopersicon esculentum , var. Volgogradskij (NaCl‐sensitive tomato) and L. pimpinellifolium , acc. PE2 (NaCl‐tolerant species)”
e7“after salt stress, a modification of the redox state of NAD kinase seems to be responsible for the inhibition of the enzyme”
The same questions in other papers
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1 paper in the same direction as this paper
- negative
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5 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
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4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.