Abstract
Rapeseed (Brassica napus) is an important oil crop worldwide. However, severe inhibition of rapeseed production often occurs in the field due to nitrogen (N) deficiency. The root system is the main organ to acquire N for plant growth, but little is known about the mechanisms underlying rapeseed root adaptions to N deficiency. Here, dynamic changes in root architectural traits of N-deficient rapeseed plants were evaluated by 3D in situ quantification. Root proteome responses to N deficiency were analyzed by the tandem mass tag-based proteomics method, and related proteins were characterized further. Under N deficiency, rapeseed roots become longer, with denser cells in the meristematic zone and larger cells in the elongation zone of root tips, and also become softer with reduced solidity. A total of 171 and 755 differentially expressed proteins were identified in short- and long-term N-deficient roots, respectively. The abundance of proteins involved in cell wall organization or biogenesis was highly enhanced, but most identified peroxidases were reduced in the N-deficient roots. Notably, peroxidase activities also were decreased, which might promote root elongation while lowering the solidity of N-deficient roots. These results were consistent with the cell wall components measured in the N-deficient roots. Further functional analysis using transgenic Arabidopsis (Arabidopsis thaliana) plants demonstrated that the two root-related differentially expressed proteins contribute to the enhanced root growth under N deficiency conditions. These results provide insights into the global changes of rapeseed root responses to N deficiency and may facilitate the development of rapeseed cultivars with high N use efficiency through root-based genetic improvements.
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Claims 12
Claim · model-written summary
Nitrogen-deficient rapeseed roots became longer.
Not reportedComparator
Verbatim
e0“Under N deficiency, rapeseed roots become longer”
Claim · model-written summary
Nitrogen-deficient root meristems had denser cells.
- Taxon
- rapeseede0
- Organ / stage
- meristematic zonee0
- Treatment
- N deficiencye0
- Outcome
- denser cellse0
- Direction
- densere0
Not reportedComparator
Verbatim
e0“Under N deficiency, rapeseed roots become longer, with denser cells in the meristematic zone”
Claim · model-written summary
Nitrogen-deficient root tips had larger elongation-zone cells.
- Taxon
- rapeseede1
- Organ / stage
- elongation zone of root tipse0
- Treatment
- N deficiencye1
- Outcome
- cellse0
- Direction
- largere0
Not reportedComparator
Verbatim
e0“larger cells in the elongation zone of root tips”
e1“Under N deficiency, rapeseed roots”
Claim · model-written summary
Nitrogen-deficient roots became softer.
Not reportedComparator
Verbatim
e0“and also become softer with reduced solidity”
e1“Under N deficiency, rapeseed roots”
Claim · model-written summary
Nitrogen-deficient roots had reduced solidity.
Not reportedComparator
Verbatim
e0“and also become softer with reduced solidity”
e1“Under N deficiency, rapeseed roots”
Claim · model-written summary
Short-term nitrogen deficiency changed abundance of 171 root proteins.
Verbatim
e0“A total of 171 and 755 differentially expressed proteins were identified in short- and long-term N-deficient roots, respectively”
e1“rapeseed plants”
Claim · model-written summary
Long-term nitrogen deficiency changed abundance of 755 root proteins.
Verbatim
e0“A total of 171 and 755 differentially expressed proteins were identified in short- and long-term N-deficient roots, respectively”
e1“rapeseed plants”
Claim · model-written summary
Cell-wall organization or biogenesis proteins increased under nitrogen deficiency.
- Taxon
- rapeseede2
- Treatment
- N-deficiente1
- Outcome
- abundance of proteins involved in cell wall organization or biogenesise0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“The abundance of proteins involved in cell wall organization or biogenesis was highly enhanced”
e1“but most identified peroxidases were reduced in the N-deficient roots”
e2“rapeseed plants”
Claim · model-written summary
Most identified peroxidases declined under nitrogen deficiency.
Not reportedComparator
Verbatim
e0“most identified peroxidases were reduced in the N-deficient roots”
e1“rapeseed plants”
Claim · model-written summary
Reduced peroxidase activity may promote root elongation.
Verbatim
e0“peroxidase activities also were decreased, which might promote root elongation”
e1“rapeseed plants”
Claim · model-written summary
Reduced peroxidase activity may lower root solidity.
Verbatim
e0“peroxidase activities also were decreased, which might promote root elongation while lowering the solidity of N-deficient roots”
e1“rapeseed plants”
Claim · model-written summary
Two root-related proteins contributed to enhanced root growth under nitrogen deficiency in transgenic Arabidopsis.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- N deficiencye0
- Gene
- two root-related differentially expressed proteinse0
- Outcome
- enhanced root growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic Arabidopsis (Arabidopsis thaliana) plants demonstrated that the two root-related differentially expressed proteins contribute to the enhanced root growth under N deficiency conditions”
The same questions in other papers
nitrogen · growth · Brassica napus (oilseed rape)Positive and negative results
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This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
- positive
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See all 82 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.