Paper · 1973 · OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)

Copper toxicity in Phaseolus vulgaris L. as influenced by iron nutrition. II. Elemental and electron microprobe analysis

Ralph Daniels; Β. Esther Struckmeyer; L. A. Peterson

10
claims
21
verbatim quotes
8
citations
1
research areas
—
Not open access

Abstract

In plants grown without Fe or with Fe tartrate, 0.50 ppm Cu reduced Fe concentration only in the roots. When FeEDTA was used, Fe concentrations were higher in the roots at 0.04 and 0.50 ppm Cu than other treatments. Concentrations of Cu in the roots increased with the increase in Cu in the nutrient solution; in the shoots there was little difference. With FeEDTA, the Cu concentration in the roots and shoots at both levels of Cu was considerably less than with Fe tartrate or without Fe. Microprobe analysis showed accumulations of Fe in wall areas of some xylem elements in the midrib of leaves of plants grown with Fe tartrate-0.50 ppm Cu only. Iron did not accumulate in comparable tissues with Fe tartrate-0.04 ppm Cu and with FeEDTA-0.40 and 0.50 ppm Cu. Copper was distributed uniformly throughout the tissues but P accumulated only in wall areas of the cells. The localization of Fe and P in the same cell suggested that high Cu induced chlorosis by precipitation of Fe and Fe phosphate. 18 references, 4 figures, 2 tables.

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Claims 10

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

At 0.50 ppm Cu, root Fe concentration declined without Fe or with Fe tartrate.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Organ / stage
rootse0
Treatment
0.50 ppm Cue0
Outcome
Fe concentratione0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 19–40 · show in text ↑
e0

“In plants grown without Fe or with Fe tartrate, 0.50 ppm Cu reduced Fe concentration only in the roots.”

✓ located verbatim in the source · abstract, characters 0–103 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

With FeEDTA, root Fe concentrations at 0.04 and 0.50 ppm Cu were higher than with other treatments.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Organ / stage
rootse0
Treatment
FeEDTAe0
Outcome
Fe concentrationse0
Direction
highere0
Qualifier
0.04 and 0.50 ppm Cue0
Comparator
other treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 19–40 · show in text ↑
e0

“When FeEDTA was used, Fe concentrations were higher in the roots at 0.04 and 0.50 ppm Cu than other treatments.”

✓ located verbatim in the source · abstract, characters 104–215 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Root Cu concentrations increased with Cu in the nutrient solution.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Treatment
Cu in the nutrient solutione0
Outcome
Concentrations of Cu in the rootse0
Direction
increasede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 19–40 · show in text ↑
e0

“Concentrations of Cu in the roots increased with the increase in Cu in the nutrient solution”

✓ located verbatim in the source · abstract, characters 216–308 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

Shoot Cu concentrations differed little as nutrient-solution Cu increased.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Treatment
Cu in the nutrient solutione1
Outcome
in the shootse1
Direction
little differencee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 19–40 · show in text ↑
e0

“in the shoots there was little difference.”

✓ located verbatim in the source · abstract, characters 310–352 · show in text ↑
e1

“Concentrations of Cu in the roots increased with the increase in Cu in the nutrient solution; in the shoots there was little difference.”

✓ located verbatim in the source · abstract, characters 216–352 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

FeEDTA yielded considerably less root and shoot Cu than Fe tartrate or no Fe at both Cu levels.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Treatment
FeEDTAe0
Outcome
Cu concentration in the roots and shootse0
Direction
considerably lesse0
Qualifier
at both levels of Cue0
Comparator
Fe tartrate or without Fee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 19–40 · show in text ↑
e0

“With FeEDTA, the Cu concentration in the roots and shoots at both levels of Cu was considerably less than with Fe tartrate or without Fe.”

✓ located verbatim in the source · abstract, characters 353–490 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fe accumulated in xylem walls of leaf midribs only under Fe tartrate and 0.50 ppm Cu.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Organ / stage
midrib of leavese0
Treatment
Fe tartrate-0.50 ppm Cue0
Outcome
accumulations of Fe in wall areas of some xylem elementse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 19–40 · show in text ↑
e0

“Microprobe analysis showed accumulations of Fe in wall areas of some xylem elements in the midrib of leaves of plants grown with Fe tartrate-0.50 ppm Cu only.”

✓ located verbatim in the source · abstract, characters 491–649 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Fe did not accumulate in comparable tissues with Fe tartrate and 0.04 ppm Cu or FeEDTA at 0.40 and 0.50 ppm Cu.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Treatment
Fe tartrate-0.04 ppm Cu and with FeEDTA-0.40 and 0.50 ppm Cue0
Outcome
Iron did not accumulate in comparable tissuese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 19–40 · show in text ↑
e0

“Iron did not accumulate in comparable tissues with Fe tartrate-0.04 ppm Cu and with FeEDTA-0.40 and 0.50 ppm Cu.”

✓ located verbatim in the source · abstract, characters 650–762 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Copper was distributed uniformly through tissues.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Outcome
Copper was distributed uniformly throughout the tissuese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 19–40 · show in text ↑
e0

“Copper was distributed uniformly throughout the tissues”

✓ located verbatim in the source · abstract, characters 763–818 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Phosphorus accumulated only in cell wall areas.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Outcome
P accumulated only in wall areas of the cellse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 19–40 · show in text ↑
e0

“P accumulated only in wall areas of the cells”

✓ located verbatim in the source · abstract, characters 823–868 · show in text ↑
Mechanismhypothesisoriginal resultnegativebasis n/a

Claim · model-written summary

The authors suggest high Cu causes chlorosis through precipitation of Fe and Fe phosphate.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Treatment
high Cue0
Outcome
chlorosise0
Qualifier
precipitation of Fe and Fe phosphatee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 19–40 · show in text ↑
e0

“The localization of Fe and P in the same cell suggested that high Cu induced chlorosis by precipitation of Fe and Fe phosphate.”

✓ located verbatim in the source · abstract, characters 870–997 · show in text ↑

copper atom · nutrient: content · Phaseolus vulgaris (common bean)Results agree

6 papers: 0 positive, 6 negative or no effect

5 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.