Abstract
MicroRNAs contribute to the adaptation of plants to varying environmental conditions by affecting systemic mineral nutrient homeostasis. Copper and iron deficiencies antagonistically control the expression of Arabidopsis thaliana microRNA408 (miR408), which post-transcriptionally regulates laccase-like multicopper oxidase family members LAC3, LAC12 and LAC13. In this work, we used miR408 T-DNA insertion mutants (408-KO1 and 408-KO2) and a previously characterized transgenic line overexpressing miR408 (35S:408-14) to explore how miR408 influences copper- and iron-dependent metabolism. We observed that the altered expression of miR408 diminished plant performance and the activation of the iron-regulated genes under iron-deficient conditions. Consistently with the low expression of the miR408-target laccases, we showed that the vascular bundle lignification of the 35S:408-14 plants diminished. The decrease in the phenoloxidase and ferroxidase activities exhibited by wild-type plants under iron deficiency did not occur in the 408-KO1 plants, probably due to the higher expression of laccases. Finally, we observed that the hydrogen peroxide levels under iron starvation were altered in both the 408-KO1 and 35S:408-14 lines. Taken together, these results suggest that Arabidopsis plants with modified miR408 levels undergo multiple deregulations under iron-deficient conditions
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Claims 9
Claim · model-written summary
Altered miR408 expression diminished Arabidopsis plant performance under iron deficiency.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- altered expression of miR408e0
- Gene
- miR408e0
- Outcome
- plant performancee0
- Direction
- diminishede0
- Qualifier
- under iron-deficient conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the altered expression of miR408 diminished plant performance and the activation of the iron-regulated genes under iron-deficient conditions.”
t0“Arabidopsis”
Claim · model-written summary
Altered miR408 expression diminished activation of iron-regulated genes under iron deficiency.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- altered expression of miR408e0
- Gene
- miR408e0
- Outcome
- activation of the iron-regulated genese0
- Direction
- diminishede0
- Qualifier
- under iron-deficient conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the altered expression of miR408 diminished plant performance and the activation of the iron-regulated genes under iron-deficient conditions.”
t0“Arabidopsis”
Claim · model-written summary
Vascular bundle lignification diminished in the miR408-overexpressing 35S:408-14 line.
- Taxon
- ArabidopsisArabidopsist0
- Material
- 35S:408-14e0
- Treatment
- overexpressing miR408e1
- Gene
- miR408e1
- Outcome
- vascular bundle lignificatione0
- Direction
- diminishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the vascular bundle lignification of the 35S:408-14 plants diminished.”
e1“transgenic line overexpressing miR408 (35S:408-14)”
t0“Arabidopsis”
Claim · model-written summary
Wild-type Arabidopsis phenoloxidase and ferroxidase activities decreased under iron deficiency.
- Taxon
- ArabidopsisArabidopsist0
- Material
- wild-typee0
- Treatment
- iron deficiencye0
- Outcome
- phenoloxidase and ferroxidase activitiese0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The decrease in the phenoloxidase and ferroxidase activities exhibited by wild-type plants under iron deficiency did not occur in the 408-KO1 plants”
t0“Arabidopsis”
Claim · model-written summary
Iron deficiency did not decrease phenoloxidase and ferroxidase activities in 408-KO1 as it did in wild type.
- Taxon
- ArabidopsisArabidopsist0
- Material
- 408-KO1e0
- Treatment
- iron deficiencye0
- Outcome
- phenoloxidase and ferroxidase activitiese0
- Direction
- did not occure0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The decrease in the phenoloxidase and ferroxidase activities exhibited by wild-type plants under iron deficiency did not occur in the 408-KO1 plants”
t0“Arabidopsis”
Claim · model-written summary
Higher laccase expression probably explains the absence of activity decreases in 408-KO1.
- Taxon
- ArabidopsisArabidopsist0
- Material
- 408-KO1e0
- Gene
- laccasese0
- Outcome
- phenoloxidase and ferroxidase activitiese0
- Qualifier
- probably due to the higher expression of laccasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The decrease in the phenoloxidase and ferroxidase activities exhibited by wild-type plants under iron deficiency did not occur in the 408-KO1 plants, probably due to the higher expression of laccases.”
t0“Arabidopsis”
Claim · model-written summary
Hydrogen peroxide levels were altered under iron starvation in 408-KO1.
- Taxon
- ArabidopsisArabidopsist0
- Material
- 408-KO1e0
- Treatment
- iron starvatione0
- Outcome
- hydrogen peroxide levelse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the hydrogen peroxide levels under iron starvation were altered in both the 408-KO1 and 35S:408-14 lines.”
t0“Arabidopsis”
Claim · model-written summary
Hydrogen peroxide levels were altered under iron starvation in 35S:408-14.
- Taxon
- ArabidopsisArabidopsist0
- Material
- 35S:408-14e0
- Treatment
- iron starvatione0
- Outcome
- hydrogen peroxide levelse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the hydrogen peroxide levels under iron starvation were altered in both the 408-KO1 and 35S:408-14 lines.”
t0“Arabidopsis”
Claim · model-written summary
Modified miR408 levels suggest multiple deregulations under iron deficiency in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- miR408e0
- Outcome
- multiple deregulationse0
- Qualifier
- under iron-deficient conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these results suggest that Arabidopsis plants with modified miR408 levels undergo multiple deregulations under iron-deficient conditions”
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