Abstract
MicroRNAs (miRNAs) are a class of non-coding RNAs that play important roles in plant development and abiotic stresses. To date, studies have mainly focused on the roles of individual miRNAs, however, a few have addressed the interactions among multiple miRNAs. In this study, we investigated the interplay and regulatory circuit between miR160 and miR165/166 and its effect on leaf development and drought tolerance in Arabidopsis using Short Tandem Target Mimic (STTM). By crossing STTM160 Arabidopsis with STTM165/166, we successfully generated a double mutant of miR160 and miR165/166. The double mutant plants exhibited a series of compromised phenotypes in leaf development and drought tolerance in comparison to phenotypic alterations in the single STTM lines. RNA-seq and qRT-PCR analyses suggested that the expression levels of auxin and ABA signaling genes in the STTM-directed double mutant were compromised compared to the two single mutants. Our results also suggested that miR160-directed regulation of auxin response factors (ARFs) contribute to leaf development via auxin signaling genes, whereas miR165/166- mediated HD-ZIP IIIs regulation confers drought tolerance through ABA signaling. Our studies further indicated that ARFs and HD-ZIP IIIs may play opposite roles in the regulation of leaf development and drought tolerance that can be further applied to other crops for agronomic traits improvement.
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Claims 8
Claim · model-written summary
The STTM160 and STTM165/166 cross produced a double mutant.
- Taxon
- Arabidopsis
Arabidopsise0 - Outcome
- double mutant of miR160 and miR165/166e0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By crossing STTM160 Arabidopsis with STTM165/166, we successfully generated a double mutant of miR160 and miR165/166.”
Claim · model-written summary
The miR160 and miR165/166 double mutant had compromised leaf development relative to single STTM lines.
- Material
- double mutant plantse0
- Outcome
- leaf developmente0
- Direction
- compromisede0
- Comparator
- single STTM linese0
Not reportedTaxon
Verbatim
e0“The double mutant plants exhibited a series of compromised phenotypes in leaf development and drought tolerance in comparison to phenotypic alterations in the single STTM lines.”
Claim · model-written summary
The miR160 and miR165/166 double mutant had compromised drought tolerance relative to single STTM lines.
- Material
- double mutant plantse0
- Outcome
- drought tolerancee0
- Direction
- compromisede0
- Comparator
- single STTM linese0
Not reportedTaxon
Verbatim
e0“The double mutant plants exhibited a series of compromised phenotypes in leaf development and drought tolerance in comparison to phenotypic alterations in the single STTM lines.”
Claim · model-written summary
Auxin signaling gene expression was compromised in the double mutant relative to single mutants.
- Material
- STTM-directed double mutante0
- Outcome
- expression levels of auxine0
- Direction
- compromisede0
- Comparator
- two single mutantse0
Not reportedTaxon
Verbatim
e0“the expression levels of auxin and ABA signaling genes in the STTM-directed double mutant were compromised compared to the two single mutants.”
Claim · model-written summary
ABA signaling gene expression was compromised in the double mutant relative to single mutants.
- Material
- STTM-directed double mutante0
- Outcome
- ABA signaling genese0
- Direction
- compromisede0
- Comparator
- two single mutantse0
Not reportedTaxon
Verbatim
e0“the expression levels of auxin and ABA signaling genes in the STTM-directed double mutant were compromised compared to the two single mutants.”
Claim · model-written summary
miR160 regulation of ARFs contributed to leaf development through auxin signaling genes.
Not reportedTaxon
Verbatim
e0“miR160-directed regulation of auxin response factors (ARFs) contribute to leaf development via auxin signaling genes”
Claim · model-written summary
miR165/166 mediated HD-ZIP III regulation conferred drought tolerance through ABA signaling.
Not reportedTaxon
Verbatim
e0“miR165/166- mediated HD-ZIP IIIs regulation confers drought tolerance through ABA signaling”
Claim · model-written summary
ARFs and HD-ZIP IIIs may have opposing roles in leaf development and drought tolerance.
- Gene
- ARFs and HD-ZIP IIIse0
- Outcome
- leaf development and drought tolerancee0
- Qualifier
- may play opposite rolese0
Not reportedTaxon
Verbatim
e0“ARFs and HD-ZIP IIIs may play opposite roles in the regulation of leaf development and drought tolerance”