Abstract
Fusarium Head Blight (FHB) is the number one floral disease of cereals and poses a serious health hazard by contaminating grain with the harmful mycotoxin deoxynivalenol (DON). Fungi adapt to fluctuations in their environment, coordinating development and metabolism accordingly. G-protein coupled receptors (GPCRs) communicate changes in the environment to intracellular G-proteins that direct the appropriate biological response, suggesting that fungal GPCR signalling may be key to virulence. Here we describe the expansion of non-classical GPCRs in the FHB causing pathogen, Fusarium graminearum, and show that class X receptors are highly expressed during wheat infection. We identify class X receptors that are required for FHB disease on wheat, and show that the absence of a GPCR can cause an enhanced host response that restricts the progression of infection. Specific receptor sub-domains are required for virulence. These non-classical receptors physically interact with intracellular G-proteins and are therefore bona fide GPCRs. Disrupting a class X receptor is shown to dysregulate the transcriptional coordination of virulence traits during infection. This amounts to enhanced wheat defensive responses, including chitinase and plant cell wall biosynthesis, resulting in apoplastic and vascular occlusions that impede infection. Our results show that GPCR signalling is important to FHB disease establishment.
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Claims 8
Claim · model-written summary
Non-classical GPCRs are expanded in Fusarium graminearum.
- Taxon
- wheat
Triticum aestivumt0 - Interactor
- Fusarium graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume0pathogen - Gene
- non-classical GPCRse0
- Outcome
- expansion of non-classical GPCRse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“the expansion of non-classical GPCRs in the FHB causing pathogen, Fusarium graminearum”
t0“Fusarium head blight on wheat”
Claim · model-written summary
Class X receptors were required for Fusarium head blight disease on wheat.
- Taxon
- wheatTriticum aestivume0
- Gene
- class X receptorse0
- Outcome
- FHB diseasee0
FusariumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“class X receptors that are required for FHB disease on wheat”
t0“Fusarium head blight”
Claim · model-written summary
Absence of a GPCR enhanced the wheat host response and restricted infection progression.
- Taxon
- wheatTriticum aestivumt0
- Gene
- GPCRe0
- Outcome
- enhanced host response that restricts the progression of infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the absence of a GPCR can cause an enhanced host response that restricts the progression of infection”
t0“Fusarium head blight on wheat”
Claim · model-written summary
Specific receptor sub-domains were required for virulence.
- Taxon
- wheatTriticum aestivumt0
- Gene
- receptor sub-domainse0
- Outcome
- virulencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Specific receptor sub-domains are required for virulence”
t0“Fusarium head blight on wheat”
Claim · model-written summary
The non-classical receptors physically interacted with intracellular G-proteins.
- Taxon
- wheatTriticum aestivumt0
- Gene
- non-classical receptorse0
- Outcome
- physically interact with intracellular G-proteinse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These non-classical receptors physically interact with intracellular G-proteins”
t0“Fusarium head blight on wheat”
Claim · model-written summary
Disrupting a class X receptor dysregulated transcriptional coordination of virulence traits during infection.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Disrupting a class X receptore0
- Gene
- class X receptore0
- Outcome
- transcriptional coordination of virulence traitse0
- Direction
- dysregulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disrupting a class X receptor is shown to dysregulate the transcriptional coordination of virulence traits during infection”
t0“Fusarium head blight on wheat”
Claim · model-written summary
Receptor disruption enhanced wheat defensive responses, including chitinase and cell wall biosynthesis.
- Taxon
- wheatTriticum aestivume0
- Gene
- chitinasee0
- Outcome
- defensive responsese0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced wheat defensive responses, including chitinase and plant cell wall biosynthesis”
t0“Fusarium head blight”
Claim · model-written summary
Wheat defensive responses produced apoplastic and vascular occlusions that impeded infection.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- apoplastic and vascular occlusions that impede infectione0
- Qualifier
- apoplastic and vascular occlusionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resulting in apoplastic and vascular occlusions that impede infection”
t0“Fusarium head blight on wheat”