Abstract
Asparagine synthetase (AS), a key enzyme in plant nitrogen metabolism, plays an important role in plant nitrogen assimilation and distribution. Asparagine (Asn), the product of asparagine synthetase, is one of the main compounds responsible for organic nitrogen transport and storage in plants. In this study, we performed complementation experiments using an Asn-deficient Escherichia coli strain to demonstrate that three putative asparagine synthetase family members in poplar (Populus simonii × P. nigra) function in Asn synthesis. Quantitative real-time PCR revealed that the three members had high expression levels in different tissues of poplar and were regulated by exogenous nitrogen. PnAS1 and PnAS2 were also affected by diurnal rhythm. Long-term dark treatment resulted in a significant increase in PnAS1 and PnAS3 expression levels. Under long-term light conditions, however, PnAS2 expression decreased significantly in the intermediate region of leaves. Exogenous application of ammonium nitrogen, glutamine, and a glutamine synthetase inhibitor revealed that PnAS3 was more sensitive to exogenous glutamine, while PnAS1 and PnAS2 were more susceptible to exogenous ammonium nitrogen. Our results suggest that the various members of the PnAS gene family have distinct roles in different tissues and are regulated in different ways.
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Claims 8
Claim · model-written summary
Three poplar asparagine synthetase family members function in asparagine synthesis.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three putative asparagine synthetase family members in poplar (Populus simonii × P. nigra) function in Asn synthesis”
Claim · model-written summary
The three members had high expression in different poplar tissues.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the three members had high expression levels in different tissues of poplar”
Claim · model-written summary
Exogenous nitrogen regulated expression of the three members.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the three members had high expression levels in different tissues of poplar and were regulated by exogenous nitrogen”
Claim · model-written summary
PnAS1 and PnAS2 expression was affected by diurnal rhythm.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PnAS1 and PnAS2 were also affected by diurnal rhythm”
t0“Poplar”
Claim · model-written summary
Long-term dark treatment significantly increased PnAS1 and PnAS3 expression.
- Treatment
- Long-term dark treatmente0
- Gene
- PnAS1 and PnAS3e0
- Outcome
- expression levelse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Long-term dark treatment resulted in a significant increase in PnAS1 and PnAS3 expression levels”
t0“Poplar”
Claim · model-written summary
Under long-term light, PnAS2 expression significantly decreased in the intermediate leaf region.
- Organ / stage
- intermediate region of leavese0
- Treatment
- long-term light conditionse0
- Gene
- PnAS2e0
- Outcome
- expressione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under long-term light conditions, however, PnAS2 expression decreased significantly in the intermediate region of leaves”
t0“Poplar”
Claim · model-written summary
PnAS3 was more sensitive to exogenous glutamine.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PnAS3 was more sensitive to exogenous glutamine”
t0“Poplar”
Claim · model-written summary
PnAS1 and PnAS2 were more susceptible to exogenous ammonium nitrogen.
- Treatment
- exogenous ammonium nitrogene0
- Gene
- PnAS1 and PnAS2e0
- Outcome
- susceptiblee0
- Direction
- more susceptiblee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PnAS1 and PnAS2 were more susceptible to exogenous ammonium nitrogen”
t0“Poplar”
The same questions in other papers
light · molecular: expression · Populus (poplar)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.