Abstract
Shade avoidance signaling involves perception of incident red/far-red (R/FR) light by phytochromes (PHYs) and modulation of downstream transcriptional networks. Although these responses are well studied in Arabidopsis, little is known about the role of PHYs and the transcriptional responses to shade in the woody perennial Populus. Tissue expression and subcellular localization of Populus PHYs was studied by quantitative real-time PCR (qRT-PCR) and protoplast transient assay. Transgenic lines with altered PHYB1 and/or PHYB2 expression were used in phenotypic assays and transcript profiling with qRT-PCR. RNA-Seq was used to identify transcriptional responses to enriched FR light. All three PHYs were differentially expressed among tissue types and PHYBs were targeted to the nucleus under white light. Populus PHYB1 rescued Arabidopsis phyB mutant phenotypes. Phenotypes of Populus transgenic lines and the expression of candidate shade response genes suggested that PHYB1 and PHYB2 have distinct yet overlapping functions. RNA-Seq analysis indicated that genes associated with cell wall modification and brassinosteroid signaling were induced under enriched FR light in Populus. This study is an initial attempt at deciphering the role of Populus PHYs by evaluating transcriptional reprogramming to enriched FR and demonstrates functional diversity and overlap of the Populus PHYB1 and PHYB2 in regulating shade responses.
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Claims 6
Claim · model-written summary
Three Populus phytochromes showed differential expression among tissues.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All three PHYs were differentially expressed among tissue types”
t0“Populus”
Claim · model-written summary
Populus PHYBs targeted the nucleus in white light.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PHYBs were targeted to the nucleus under white light”
t0“Populus”
Claim · model-written summary
Populus PHYB1 rescued Arabidopsis phyB mutant phenotypes.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Populus PHYB1e0
- Gene
- Populus PHYB1e0
- Outcome
- phyB mutant phenotypese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Populus PHYB1 rescued Arabidopsis phyB mutant phenotypes”
Claim · model-written summary
Populus PHYB1 and PHYB2 had distinct but overlapping functions.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PHYB1 and PHYB2 have distinct yet overlapping functions”
t0“Populus”
Claim · model-written summary
Enriched far-red light induced genes associated with cell wall modification.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes associated with cell wall modification and brassinosteroid signaling were induced under enriched FR light in Populus”
Claim · model-written summary
Enriched far-red light induced genes associated with brassinosteroid signaling.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes associated with cell wall modification and brassinosteroid signaling were induced under enriched FR light in Populus”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.