Abstract
Bark storage proteins accumulate in the bark of many woody plants during autumn and winter. In poplar (Populus deltoides Bartr. ex Marsh), the accumulation of the 32-kilodalton bark storage protein is controlled by photoperiod. We have isolated a full-length cDNA encoding for the poplar 32-kilodalton bark storage protein and determined its nucleotide sequence. The derived amino acid sequence shows that poplar bark storage protein is rich in serine, leucine, phenylalanine, and lysine. Poplar bark storage protein is similar to the poplar wound-induced cDNA clone 4 and clone 16 (TJ Parsons, HD Bradshaw, MP Gordon [1989] Proc Natl Acad Sci USA 86: 7895-7899). DNA gel blot analysis suggests that poplar bark storage protein is encoded by a multigene family of about five genes. Poplar plants grown in long days contained low levels of mRNA for the bark storage protein. Exposure to short days resulted in an increase in bark storage protein mRNA within 7 days. After 21 days of short day exposure, high levels of mRNA were detected. The accumulation of bark storage protein mRNA in response to short days was also observed in plants exposed to natural shortening daylengths. Our results indicate that the accumulation of poplar bark storage protein mRNA is controlled by photoperiod. This finding will provide a useful system for investigating photoperiodism in woody plants.
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Claims 9
Claim · model-written summary
A full-length cDNA encoding poplar 32-kilodalton bark storage protein was isolated and sequenced.
PopulusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have isolated a full-length cDNA encoding for the poplar 32-kilodalton bark storage protein and determined its nucleotide sequence.”
e1“Poplar plants grown in long days contained low levels of mRNA for the bark storage protein.”
Claim · model-written summary
The derived protein is rich in serine, leucine, phenylalanine, and lysine.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The derived amino acid sequence shows that poplar bark storage protein is rich in serine, leucine, phenylalanine, and lysine.”
e1“Poplar plants grown in long days contained low levels of mRNA for the bark storage protein.”
Claim · model-written summary
Poplar bark storage protein resembles wound-induced cDNA clones 4 and 16.
- Gene
- Poplar bark storage proteine0
- Outcome
- similare0
- Comparator
- poplar wound-induced cDNA clone 4 and clone 16e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Poplar bark storage protein is similar to the poplar wound-induced cDNA clone 4 and clone 16”
e1“Poplar plants grown in long days contained low levels of mRNA for the bark storage protein.”
Claim · model-written summary
DNA blotting suggested a bark storage protein multigene family of about five genes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA gel blot analysis suggests that poplar bark storage protein is encoded by a multigene family of about five genes.”
e1“Poplar plants grown in long days contained low levels of mRNA for the bark storage protein.”
Claim · model-written summary
Long-day poplars had low bark storage protein mRNA.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Poplar plants grown in long days contained low levels of mRNA for the bark storage protein.”
e1“Poplar plants grown in long days contained low levels of mRNA for the bark storage protein.”
Claim · model-written summary
Short days increased bark storage protein mRNA within seven days.
- Treatment
- short dayse0
- Gene
- bark storage proteine0
- Outcome
- mRNAe0
- Direction
- increasee0
- Value
- 7 dayse0
- Comparator
- long dayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure to short days resulted in an increase in bark storage protein mRNA within 7 days.”
e1“Poplar plants grown in long days contained low levels of mRNA for the bark storage protein.”
Claim · model-written summary
Bark storage protein mRNA was high after 21 short days.
- Treatment
- short day exposuree0
- Gene
- bark storage proteine1
- Outcome
- mRNAe0
- Direction
- high levelse0
- Value
- 21 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 21 days of short day exposure, high levels of mRNA were detected.”
e1“Poplar plants grown in long days contained low levels of mRNA for the bark storage protein.”
Claim · model-written summary
Natural shortening daylengths also induced bark storage protein mRNA accumulation.
- Treatment
- natural shortening daylengthse0
- Gene
- bark storage proteine0
- Outcome
- mRNAe0
- Direction
- accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The accumulation of bark storage protein mRNA in response to short days was also observed in plants exposed to natural shortening daylengths.”
e1“Poplar plants grown in long days contained low levels of mRNA for the bark storage protein.”
Claim · model-written summary
The authors conclude that photoperiod controls bark storage protein mRNA accumulation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results indicate that the accumulation of poplar bark storage protein mRNA is controlled by photoperiod.”
e1“Poplar plants grown in long days contained low levels of mRNA for the bark storage protein.”
The same questions in other papers
light · molecular: expression · Populus (poplar)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.