Abstract
Abstract Hairy roots cultures resulting from transformation by the soil bacteria Rhizobium rhizogenes represent a biotechnological tool to produce specialized bioactive metabolites. We previously developed a protocol allowing the sustainable production of hairy roots from hybrid poplar 717-1B4 ( Populus tremula × Populus alba ). In the present study we assessed physicochemical parameters for the optimization of growth of hybrid poplar HP15-3 hairy root line and production of ethyl acetate extracts with antioxidant potential. The use of Woody Plant Medium (WPM) as mineral base significantly improved growth index by 2.1-fold compared to Murashige and Skoog (MS) medium and was selected even if a slight decrease in total phenolic content (TPC) and antioxidant activity could be observed (DPPH and FRAP assays). Among the different concentrations tested (15, 30 and 60 g·L −1 ), sucrose at 30 g·L −1 made it possible to significantly increase the growth index, without affecting the TPC and the antioxidant potential of the root extracts. Stressful conditions likely to affect the biosynthesis of specialized metabolites were also tested. The cultivation of hairy roots under LED light conditions resulted in a significant 2.45-fold increase in growth index. Antioxidant activity was also increased in relation to increased anthocyanin production. Furthermore, we demonstrated the feasibility of poplar hairy roots biomass production in temporary immersion bioreactors (RITA ® ) under two water and mineral supply conditions. UHPLC/HRMS analysis of the extracts allowed to identify and quantify two phenolic glycosides (tremulacin and nigracin) whose concentrations were increased during cultivation under LED light. Two flavonoids (cyanidin and cyanidin-3-glucoside) were identified in light-grown root extracts. The parameters optimized during this research work could be successfully transposed to produce root biomass in bioreactors and under light to obtain bioactive ingredients for cosmetic formulations.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
WPM improved hairy-root growth index 2.1-fold relative to MS medium.
- Treatment
- Woody Plant Medium (WPM)e0
- Outcome
- growth indexe0
- Direction
- improvede0
- Value
- 2.1-folde0
- Comparator
- Murashige and Skoog (MS) mediume0
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of Woody Plant Medium (WPM) as mineral base significantly improved growth index by 2.1-fold compared to Murashige and Skoog (MS) medium”
t0“hybrid poplar (Populus tremula × Populus alba)”
Claim · model-written summary
WPM slightly decreased total phenolic content and antioxidant activity.
- Treatment
- Woody Plant Medium (WPM)e1
- Outcome
- total phenolic content (TPC) and antioxidant activitye0
- Direction
- decreasee0
- Comparator
- Murashige and Skoog (MS) mediume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a slight decrease in total phenolic content (TPC) and antioxidant activity could be observed (DPPH and FRAP assays)”
e1“The use of Woody Plant Medium (WPM) as mineral base significantly improved growth index by 2.1-fold compared to Murashige and Skoog (MS) medium”
t0“hybrid poplar (Populus tremula × Populus alba)”
Claim · model-written summary
Sucrose at 30 g·L −1 significantly increased hairy-root growth index.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose at 30 g·L −1 made it possible to significantly increase the growth index”
t0“hybrid poplar (Populus tremula × Populus alba)”
Claim · model-written summary
Sucrose at 30 g·L −1 did not affect total phenolic content or antioxidant potential.
- Treatment
- sucrose at 30 g·L −1e0
- Outcome
- TPC and the antioxidant potentiale0
- Direction
- without affectinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose at 30 g·L −1 made it possible to significantly increase the growth index, without affecting the TPC and the antioxidant potential of the root extracts”
t0“hybrid poplar (Populus tremula × Populus alba)”
Claim · model-written summary
LED light increased hairy-root growth index 2.45-fold.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cultivation of hairy roots under LED light conditions resulted in a significant 2.45-fold increase in growth index”
t0“hybrid poplar (Populus tremula × Populus alba)”
Claim · model-written summary
Antioxidant activity increased with anthocyanin production.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antioxidant activity was also increased in relation to increased anthocyanin production”
t0“hybrid poplar (Populus tremula × Populus alba)”
Claim · model-written summary
Poplar hairy-root biomass production was feasible in temporary immersion bioreactors under two supply conditions.
- Outcome
- feasibility of poplar hairy roots biomass productione0
- Value
- two water and mineral supply conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“feasibility of poplar hairy roots biomass production in temporary immersion bioreactors (RITA ® ) under two water and mineral supply conditions”
Claim · model-written summary
Tremulacin and nigracin concentrations rose under LED light.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two phenolic glycosides (tremulacin and nigracin) whose concentrations were increased during cultivation under LED light”
t0“hybrid poplar (Populus tremula × Populus alba)”
Claim · model-written summary
Cyanidin and cyanidin-3-glucoside were identified in light-grown root extracts.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two flavonoids (cyanidin and cyanidin-3-glucoside) were identified in light-grown root extracts”
t0“hybrid poplar (Populus tremula × Populus alba)”
The same questions in other papers
light · growth · Populus (poplar)Positive and negative results
5 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
4 papers more in this group
See all 8 papers →in vitro · growth · Populus (poplar)Positive and negative results
12 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
11 papers more in this group
See all 15 papers →light · molecular: metabolites · Populus (poplar)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.