Abstract
Trehalose-6-phosphate (T6P) phosphatase (TPP), a dephosphorylating enzyme, catalyzes the dephosphorylation of T6P, generating trehalose. In Jatropha, we found six members of the TPP family. Five of them JcTPPA, JcTPPC, JcTPPD, JcTPPG, and JcTPPJ are highly expressed in female flowers or male flowers, or both, suggesting that members of the JcTPP family may participate in flower development in Jatropha. The wide expression of JcTPPJ gene in various organs implied its versatile roles and thus was chosen for unraveling its biological functions during developmental process. We constructed an overexpression vector of JcTPPJ cDNA driven by the cauliflower mosaic virus (CaMV) 35S promoter for genetic transformation. Compared with control Arabidopsis plants, 35S:JcTPPJ transgenic Arabidopsis plants presented greater sucrose contents in their inflorescences and displayed late-flowering and heterostylous phenotypes. Exogenous application of sucrose to the inflorescence buds of wild-type Arabidopsis repressed the development of the perianth and filaments, with a phenocopy of the 35S:JcTPPJ transgenic Arabidopsis. These results suggested that the significantly increased sucrose level in the inflorescence caused (or induced) by JcTTPJ overexpression, was responsible for the formation of heterostylous flower phenotype. However, 35S:JcTPPJ transgenic Jatropha displayed no obvious phenotypic changes, implying that JcTPPJ alone may not be sufficient for regulating flower development in Jatropha. Our results are helpful for understanding the function of TPPs, which may regulate flower organ development by manipulating the sucrose status in plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Six TPP family members were found in Jatropha.
JatrophaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Jatropha, we found six members of the TPP family.”
Claim · model-written summary
Five JcTPP genes were highly expressed in flowers.
- Organ / stage
- female flowers or male flowerse0
- Gene
- JcTPPA, JcTPPC, JcTPPD, JcTPPG, and JcTPPJe0
- Outcome
- highly expressed in female flowers or male flowers, or bothe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Five of them JcTPPA, JcTPPC, JcTPPD, JcTPPG, and JcTPPJ are highly expressed in female flowers or male flowers, or both”
t0“Jatropha curcas”
Claim · model-written summary
JcTPPJ was expressed across various organs.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The wide expression of JcTPPJ gene in various organs”
t0“Jatropha curcas”
Claim · model-written summary
JcTPPJ overexpression raised inflorescence sucrose relative to controls in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- inflorescencese0
- Treatment
- 35S:JcTPPJ transgenic Arabidopsis plantse0
- Gene
- JcTPPJe0
- Outcome
- sucrose contentse0
- Direction
- greatere0
- Comparator
- control Arabidopsis plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with control Arabidopsis plants, 35S:JcTPPJ transgenic Arabidopsis plants presented greater sucrose contents in their inflorescences”
Claim · model-written summary
JcTPPJ overexpression caused late flowering and heterostyly in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- 35S:JcTPPJ transgenic Arabidopsis plantse0
- Gene
- JcTPPJe0
- Outcome
- late-flowering and heterostylous phenotypese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“35S:JcTPPJ transgenic Arabidopsis plants presented greater sucrose contents in their inflorescences and displayed late-flowering and heterostylous phenotypes”
Claim · model-written summary
Applied sucrose repressed perianth and filament development in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- inflorescence budse0
- Treatment
- Exogenous application of sucrosee0
- Outcome
- development of the perianth and filamentse0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of sucrose to the inflorescence buds of wild-type Arabidopsis repressed the development of the perianth and filaments”
Claim · model-written summary
Applied sucrose phenocopied the transgenic Arabidopsis flowers.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- phenocopy of the 35S:JcTPPJ transgenic Arabidopsise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with a phenocopy of the 35S:JcTPPJ transgenic Arabidopsis”
Claim · model-written summary
JcTPPJ overexpression produced no obvious phenotype change in Jatropha.
- Treatment
- 35S:JcTPPJ transgenic Jatrophae0
- Gene
- JcTPPJe0
- Outcome
- phenotypic changese0
- Direction
- no obviouse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“35S:JcTPPJ transgenic Jatropha displayed no obvious phenotypic changes”
The same questions in other papers
sucrose · growth · ArabidopsisPositive and negative results
7 papers in the same direction as this paper · 9 in the opposite direction
- positive
- negative
- positive
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.