Abstract
growth and development of anthers of two soybean cultivars, Merubetiri and Wilis cultured in vitro. The trial was conducted at the Plant Biotechnology Laboratory, Agricultural Faculty, University of Jambi. Anthers were cultured on MS solid medium provided with IAA, 2,4-D or NAA as auxin source in combination with BAP or kinetin as cytokinin source. Each growth regulators was tested at 0, 5, 10, 15 and 20 μM. The experiment was placed in a completely randomized design with five replicates. Each replicates consisted of 8 to 10 anthers obtained from the same floral bud. Cultures were placed in a light intensity of 50 μmol m-2.s-1 and 16-hour photoperiod at 25±1 oC. Observation was done weekly for 8 weeks of culture. Results indicated that response showed by anthers cultured on medium supplemented with 2,4-D+BAP, IAA+BAP and NAA+BAP, in the form of callus proliferation, occurred within 5-18 days of culture initiation. Meanwhile, with 2,4-D+kinetin, IAA+kinetin and NAA+kinetin, callus proliferation took place within 4-16 days of culture initiation. Callus formation was preceded by a swollen on the surface of anthers, followed by changing in color from light green to brownish. Following this, anther wall turned into amorphous shape, before it was finally covered by a white, cream or light green callus mass. Initially, the callus showed friable or compact structure, but following two weeks of proliferation all callus showed compact structure. Among growth regulators tested, combination involving 2,4-D produced more callus than other combinations. In addition, of the two cultivars tested, Merubetiri showed better response compared to Wilis.
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Claims 10
Claim · model-written summary
Soybean anthers on 2,4-D+BAP proliferated callus within 5-18 days.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- 2,4-D+BAPe0
- Outcome
- callus proliferatione0
- Value
- 5-18 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean Anther Culture”
e0“response showed by anthers cultured on medium supplemented with 2,4-D+BAP, IAA+BAP and NAA+BAP, in the form of callus proliferation, occurred within 5-18 days of culture initiation”
Claim · model-written summary
Soybean anthers on IAA+BAP proliferated callus within 5-18 days.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- IAA+BAPe0
- Outcome
- callus proliferatione0
- Value
- 5-18 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean Anther Culture”
e0“response showed by anthers cultured on medium supplemented with 2,4-D+BAP, IAA+BAP and NAA+BAP, in the form of callus proliferation, occurred within 5-18 days of culture initiation”
Claim · model-written summary
Soybean anthers on NAA+BAP proliferated callus within 5-18 days.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- NAA+BAPe0
- Outcome
- callus proliferatione0
- Value
- 5-18 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean Anther Culture”
e0“response showed by anthers cultured on medium supplemented with 2,4-D+BAP, IAA+BAP and NAA+BAP, in the form of callus proliferation, occurred within 5-18 days of culture initiation”
Claim · model-written summary
Soybean anthers on 2,4-D+kinetin proliferated callus within 4-16 days.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 2,4-D+kinetine0
- Outcome
- callus proliferatione0
- Value
- 4-16 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean Anther Culture”
e0“with 2,4-D+kinetin, IAA+kinetin and NAA+kinetin, callus proliferation took place within 4-16 days of culture initiation”
Claim · model-written summary
Soybean anthers on IAA+kinetin proliferated callus within 4-16 days.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- IAA+kinetine0
- Outcome
- callus proliferatione0
- Value
- 4-16 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean Anther Culture”
e0“with 2,4-D+kinetin, IAA+kinetin and NAA+kinetin, callus proliferation took place within 4-16 days of culture initiation”
Claim · model-written summary
Soybean anthers on NAA+kinetin proliferated callus within 4-16 days.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- NAA+kinetine0
- Outcome
- callus proliferatione0
- Value
- 4-16 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean Anther Culture”
e0“with 2,4-D+kinetin, IAA+kinetin and NAA+kinetin, callus proliferation took place within 4-16 days of culture initiation”
Claim · model-written summary
Soybean callus formation began with swollen anther surfaces, then browning, amorphous anther walls, and callus coverage.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- antherse0
- Outcome
- Callus formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean Anther Culture”
e0“Callus formation was preceded by a swollen on the surface of anthers, followed by changing in color from light green to brownish. Following this, anther wall turned into amorphous shape, before it was finally covered by a white, cream or light green callus mass”
Claim · model-written summary
Initially friable or compact soybean callus became compact after two weeks of proliferation.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- all callus showed compact structuree0
- Qualifier
- following two weeks of proliferatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean Anther Culture”
e0“Initially, the callus showed friable or compact structure, but following two weeks of proliferation all callus showed compact structure”
Claim · model-written summary
Combinations involving 2,4-D produced more soybean callus than the other regulator combinations.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- combination involving 2,4-De0
- Outcome
- calluse0
- Direction
- moree0
- Comparator
- other combinationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean Anther Culture”
e0“Among growth regulators tested, combination involving 2,4-D produced more callus than other combinations”
Claim · model-written summary
Merubetiri soybean showed better anther culture response than Wilis.
- Taxon
- SoybeanGlycine maxt0
- Material
- Merubetirie0
- Outcome
- better responsee0
- Direction
- bettere0
- Comparator
- Wilise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean Anther Culture”
e0“of the two cultivars tested, Merubetiri showed better response compared to Wilis”
The same questions in other papers
2,4-D · growth · Glycine max (soybean)Results differ
19 papers in the same direction as this paper · 15 in the opposite direction
- negative
- negative
- negative
31 papers more in this group
See all 35 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.