Abstract
Recently, begomovirus/betasatellite disease complexes were found to be associated with alphasatellites, and their presence modulated disease symptoms and/or viral DNA accumulation in infected plants. However, the biological functions of alphasatellites during begomovirus/betasatellite infections remain unclear. Tomato yellow leaf curl China virus (TYLCCNV) associated with a betasatellite (TYLCCNB) is a widespread monopartite begomovirus in China. In the Yunnan province of China, the TYLCCNV/TYLCCNB disease complex is found in association with an alphasatellite (TYLCCNA). In this study, in order to explain the mechanisms underlying TYLCCNV/TYLCCNB infection and reductions in viral DNA accumulation caused by TYLCCNA, we analyzed the transcriptome profiles of Nicotiana benthamiana seedlings challenged by TYLCCNV/TYLCCNB or TYLCCNV/TYLCCNB/TYLCCNA using RNA sequencing. In total, 2272 and 1207 differentially expressed genes (DEGs) were identified to respond to TYLCCNV/TYLCCNB and TYLCCNV/TYLCCNB/TYLCCNA infections, respectively. Compared with the DEGs in the TYLCCNV/TYLCCNB-infected N. benthamiana seedlings, the number of DEGs in plants co-infected with TYLCCNV/TYLCCNB + TYLCCNA was significantly reduced. Additionally, 36 DEGs were identified to be regulated by TYLCCNA, six of which were further analyzed using the virus-induced gene silencing (VIGS) approach. Silencing of these six TYLCCNA responsive DEGs caused more severe disease symptoms and higher viral DNA accumulation levels, suggesting that TYLCCNA responsive DEGs may attenuate TYLCCNV/TYLCCNB infection.
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Claims 6
Claim · model-written summary
TYLCCNV/TYLCCNB infection yielded 2272 differentially expressed genes.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0t0 - Treatment
- TYLCCNV/TYLCCNBe0
- Outcome
- differentially expressed genes (DEGs)e0
- Value
- 2272e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2272 and 1207 differentially expressed genes (DEGs) were identified to respond to TYLCCNV/TYLCCNB and TYLCCNV/TYLCCNB/TYLCCNA infections, respectively.”
t0“Nicotiana benthamiana”
Claim · model-written summary
TYLCCNV/TYLCCNB/TYLCCNA infection yielded 1207 differentially expressed genes.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0t0
- Treatment
- TYLCCNV/TYLCCNB/TYLCCNAe0
- Outcome
- differentially expressed genes (DEGs)e0
- Value
- 1207e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2272 and 1207 differentially expressed genes (DEGs) were identified to respond to TYLCCNV/TYLCCNB and TYLCCNV/TYLCCNB/TYLCCNA infections, respectively.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Coinfection with TYLCCNA significantly reduced the number of host differentially expressed genes.
Verbatim
e0“the number of DEGs in plants co-infected with TYLCCNV/TYLCCNB + TYLCCNA was significantly reduced.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Thirty six host differentially expressed genes were regulated by TYLCCNA.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“36 DEGs were identified to be regulated by TYLCCNA”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing six TYLCCNA responsive genes caused more severe disease symptoms.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0t0
- Treatment
- Silencing of these six TYLCCNA responsive DEGse0
- Outcome
- disease symptomse0
- Direction
- more severee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of these six TYLCCNA responsive DEGs caused more severe disease symptoms and higher viral DNA accumulation levels”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing six TYLCCNA responsive genes increased viral DNA accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0t0
- Treatment
- Silencing of these six TYLCCNA responsive DEGse0
- Outcome
- viral DNA accumulation levelse0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of these six TYLCCNA responsive DEGs caused more severe disease symptoms and higher viral DNA accumulation levels”
t0“Nicotiana benthamiana”
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