Claim · model-written summary
Silencing NbbHLH3 increased nicotine content in N. benthamiana leaves.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Organ / stage
- leafe1
- Treatment
- VIGSe0
- Gene
- NbbHLH3e0
- Outcome
- nicotine contente0
- Direction
- increasede0
- Comparator
- control plantse0
Not reportedMaterialCompoundInteractorInteractor roleSecond interactorSecond interactor roleSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VIGS of a basic helix-loop-helix (bHLH) TF, NbbHLH3, and an auxin response factor TF, NbARF1, increased nicotine content compared with control plants; silencing the bHLH family members, NbbHLH1 and NbbHLH2, an ethylene response factor TF, NbERF1, and a homeobox domain-like TF, NbHB1, reduced nicotine levels.”
e1“After a VIGS screen to determine their effect on nicotine metabolism, six TFs from three different TF families altered constitutive and MeJA-induced leaf nicotine levels.”
t0“A functional genomics screen identifies diverse transcription factors that regulate alkaloid biosynthesis in Nicotiana benthamiana”
Title
Andrea T. Todd; Enwu Liu; Sandra L. Polvi; et al. The Plant Journal 2010. doi:10.1111/j.1365-313x.2010.04186.x
Other claims from this paper (14)
- Method / resource Sequencing produced 3271 ESTs representing 1898 unigenes, enriched for jasmonate-responsive genes.
- Observation The sequence set included 69 putative transcription factors.
- Method / resource VIGS screening found six transcription factors from three families that altered leaf nicotine levels.
- Intervention effect Silencing NbARF1 increased nicotine content in N. benthamiana leaves.
- Intervention effect Silencing NbbHLH1 reduced nicotine content in N. benthamiana leaves.
- Intervention effect Silencing NbbHLH2 reduced nicotine content in N. benthamiana leaves.
- Intervention effect Silencing NbERF1 reduced nicotine content in N. benthamiana leaves.
- Intervention effect Silencing NbHB1 reduced nicotine content in N. benthamiana leaves.
- Intervention effect Overexpression of NbbHLH1 increased leaf nicotine relative to vector controls.
- Intervention effect Overexpression of NbbHLH2 increased leaf nicotine relative to vector controls.
- Intervention effect RNAi silencing reduced nicotine levels.
- Intervention effect RNAi silencing decreased transcripts encoding nicotine pathway enzymes.
- Observation NbbHLH1 and NbbHLH2 bind G-box elements in the putrescine N-methyltransferase promoter.
- Mechanism NbbHLH1 and NbbHLH2 are positive regulators of jasmonate-induced nicotine biosynthesis.