Abstract
Brassinosteroids (BRs), plant steroid hormones, play essential roles in modulating cell elongation, vascular differentiation, senescence, and stress responses. However, the mechanisms by which BRs regulate plant mitochondria and resistance to abiotic stress remain largely unclear. Mitochondrial alternative oxidase (AOX) is involved in the plant response to a variety of environmental stresses. In this report, the role of AOX in BR-induced tolerance against cold, polyethylene glycol (PEG), and high-light stresses was investigated. Exogenous applied brassinolide (BL, the most active BR) induced, while brassinazole (BRZ, a BR biosynthesis inhibitor) reduced alternative respiration and AOX1 expression in Nicotiana benthamiana. Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis. Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance. Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.
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Claims 7
Claim · model-written summary
Brassinolide induced alternative respiration and AOX1 expression.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- brassinolidee0
- Gene
- AOX1e0
- Outcome
- alternative respiration and AOX1 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous applied brassinolide (BL, the most active BR) induced, while brassinazole (BRZ, a BR biosynthesis inhibitor) reduced alternative respiration and AOX1 expression in Nicotiana benthamiana.”
Claim · model-written summary
Brassinazole reduced alternative respiration and AOX1 expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- brassinazolee0
- Gene
- AOX1e0
- Outcome
- alternative respiration and AOX1 expressione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous applied brassinolide (BL, the most active BR) induced, while brassinazole (BRZ, a BR biosynthesis inhibitor) reduced alternative respiration and AOX1 expression in Nicotiana benthamiana.”
Claim · model-written summary
Chemical scavenging of H2O2 compromised the BR-induced alternative respiratory pathway.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Chemical scavenging of H2O2e0
- Outcome
- BR-induced alternative respiratory pathwaye0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbRBOHB compromised the BR-induced alternative respiratory pathway.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- virus-induced gene silencing (VIGS) of NbRBOHBe0
- Gene
- NbRBOHBe0
- Outcome
- BR-induced alternative respiratory pathwaye0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway”
t0“Nicotiana benthamiana”
Claim · model-written summary
AOX inhibition decreased resistance to environmental stresses and BR-induced stress tolerance.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- inhibition of AOX activity by chemical treatmente0
- Gene
- AOXe0
- Outcome
- plant resistance to environmental stresses and compromised BR-induced stress tolerancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance”
t0“Nicotiana benthamiana”
Claim · model-written summary
AOX inhibition by VIGS decreased resistance to environmental stresses and BR-induced stress tolerance.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- a VIGS-based approache0
- Gene
- AOXe0
- Outcome
- plant resistance to environmental stresses and compromised BR-induced stress tolerancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance”
t0“Nicotiana benthamiana”
Claim · model-written summary
The authors infer BR-induced AOX capability may limit reactive oxygen species accumulation and protect photosystems under stress.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Gene
- AOXe0
- Outcome
- avoidance of superfluous reactive oxygen species accumulation and the protection of photosystemse0
- Qualifier
- might contributee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana”
The same questions in other papers
hydrogen peroxide · molecular: metabolites · Nicotiana benthamianaPositive and negative results
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brassinolide · molecular: expression · Nicotiana benthamianaResults agree
1 paper in the same direction as this paper
- positive
loss of function · molecular: metabolites · Nicotiana benthamianaPositive and negative results
24 papers in the same direction as this paper · 10 in the opposite direction
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31 papers more in this group
See all 35 papers →loss of function · tolerance: abiotic · Nicotiana benthamianaResults agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.