Abstract
Numerous studies suggest that virus infections can improve drought resistance in host plants, but the underlying mechanisms remain unclear. In this study, we used Turnip mosaic virus (TuMV), Potato virus Y (PVY), Tomato bushy stunt virus (TBSV) and the host Nicotiana benthamiana as model systems to investigate these mechanisms. Our findings reveal that the abscisic acid (ABA) signalling pathway is strongly and durably induced by +RNA virus infection and is essential for virus-induced drought tolerance. Notably, although ABA content increased following virus infection, this elevation was not necessary for downstream ABA signalling or virus-induced drought tolerance. Instead, +RNA virus-induced drought tolerance relies on N. benthamiana phospholipase Dα1 (NbPLDα1)-derived phosphatidic acid (PA). Knockout of NbPLDα1 or disruption of the interaction between viral proteins and NbPLDα1 impaired the ability of +RNA viruses to activate ABA signalling and enhance drought tolerance. The virus-induced increase in ABA levels appears to result from feedback regulation by PA-activated ABA signalling. Overall, our results suggest that +RNA viruses improve plant drought tolerance by modulating NbPLDα1-derived PA rather than by promoting ABA production.
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Claims 8
Claim · model-written summary
RNA virus infection strongly and durably induced ABA signalling.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Treatment
- virus infectione0
- Outcome
- abscisic acid (ABA) signalling pathwaye0
- Direction
- inducede0
Not reportedGeneCompoundInteractorInteractor roleComparatorQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“the abscisic acid (ABA) signalling pathway is strongly and durably induced by +RNA virus infection”
Claim · model-written summary
ABA signalling was essential for virus-induced drought tolerance.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- virus-induced drought tolerancee0
- Qualifier
- essentiale0
Not reportedGeneCompoundInteractorInteractor roleTreatmentDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“is essential for virus-induced drought tolerance”
Claim · model-written summary
ABA content increased following virus infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- virus infectione0
- Outcome
- ABA contente0
- Direction
- increasede0
Not reportedGeneCompoundInteractorInteractor roleComparatorQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“ABA content increased following virus infection”
Claim · model-written summary
Elevated ABA content was unnecessary for downstream signalling or drought tolerance.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- downstream ABA signalling or virus-induced drought tolerancee0
- Qualifier
- not necessarye0
Not reportedGeneCompoundInteractorInteractor roleTreatmentDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“this elevation was not necessary for downstream ABA signalling or virus-induced drought tolerance”
Claim · model-written summary
Virus-induced drought tolerance relied on NbPLDα1-derived phosphatidic acid.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- N. benthamiana phospholipase Dα1 (NbPLDα1)e0
- Compound
- phosphatidic acid (PA)e0
- Outcome
- virus-induced drought tolerancee0
Not reportedInteractorInteractor roleTreatmentDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“+RNA virus-induced drought tolerance relies on N. benthamiana phospholipase Dα1 (NbPLDα1)-derived phosphatidic acid (PA)”
Claim · model-written summary
NbPLDα1 knockout impaired viral activation of ABA signalling and drought tolerance.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Knockout of NbPLDα1e0
- Gene
- NbPLDα1e0
- Outcome
- ability of +RNA viruses to activate ABA signalling and enhance drought tolerancee0
- Direction
- impairede0
Not reportedCompoundInteractorInteractor roleComparatorQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“Knockout of NbPLDα1 or disruption of the interaction between viral proteins and NbPLDα1 impaired the ability of +RNA viruses to activate ABA signalling and enhance drought tolerance”
Claim · model-written summary
Disrupting viral protein interaction with NbPLDα1 impaired viral activation of ABA signalling and drought tolerance.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- disruption of the interaction between viral proteins and NbPLDα1e0
- Gene
- NbPLDα1e0
- Outcome
- ability of +RNA viruses to activate ABA signalling and enhance drought tolerancee0
- Direction
- impairede0
Not reportedCompoundInteractorInteractor roleComparatorQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“disruption of the interaction between viral proteins and NbPLDα1 impaired the ability of +RNA viruses to activate ABA signalling and enhance drought tolerance”
Claim · model-written summary
The rise in ABA appeared to follow feedback from PA-activated ABA signalling.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Compound
- PAe0
- Outcome
- increase in ABA levelse0
- Qualifier
- feedback regulation by PA-activated ABA signallinge0
Not reportedGeneInteractorInteractor roleTreatmentDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“The virus-induced increase in ABA levels appears to result from feedback regulation by PA-activated ABA signalling”
The same questions in other papers
loss of function · tolerance: abiotic · Nicotiana benthamianaResults agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.