Abstract
Mung bean (Vigna radiata), an important legume crop, has the property of desiccation tolerance (DT), which is lost in the final stage of germination (preimbibition, 18 h-24 h). We compared parameters related to the programmed cell death (PCD) of mung bean seeds before and after dehydration at different imbibition stages through various detection methods. The results of Evans blue and TTC staining methods showed that the dehydration process could lead to cell death. The results of optical and subcellular morphology showed that PCD occurred after dehydration. The destruction of DNA integrity and the activity changes in caspase and total nuclease in mung bean seeds after dehydration treatment indicated that the loss of desiccation tolerance was related to PCD. Dehydration resulted in the destruction of the mitochondrial structure, reversal of the membrane potential, and the entrance of cytochrome C into the cytoplasm. These processes all indicate that the mitochondrial apoptosis pathway was the main form of dehydration-induced PCD. The results of cytoplasmic Ca2+ concentration showed that Ca2+ signaling also played a role in inducing PCD, with the upstream signal being dehydration-induced changes in water potential and the downstream signal being the ROS and mitochondrial PT channel, according to the order in which these signals happened. The mitochondrial apoptosis pathway can be considered the main mechanism of dehydration-induced PCD based on our analysis of the sequence of major events in PCD. The main processes include dehydration induction, changes in Ca2+ and mitochondrial respiratory electron transport, the reversal of mitochondrial membrane potential induced by ROS and Ca2+, and the transmission and execution of PCD downstream signals induced by cytochrome C release.
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Claims 11
Claim · model-written summary
Dehydration caused cell death in mung bean seeds.
- Taxon
- Mung bean (Vigna radiata)
.jpg?width=60)
Vigna radiatae1 - Treatment
- dehydration processe0
- Outcome
- cell deathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“the dehydration process could lead to cell death”
e1“Mung bean (Vigna radiata)”
Claim · model-written summary
Programmed cell death occurred after dehydration.
- Taxon
- Mung bean (Vigna radiata)Vigna radiatae1
- Treatment
- dehydratione0
- Outcome
- PCD occurrede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCD occurred after dehydration”
e1“Mung bean (Vigna radiata)”
Claim · model-written summary
DNA integrity was disrupted after seed dehydration.
- Taxon
- Mung bean (Vigna radiata)Vigna radiatae1
- Treatment
- dehydration treatmente0
- Outcome
- DNA integritye0
- Direction
- destructione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The destruction of DNA integrity and the activity changes in caspase and total nuclease in mung bean seeds after dehydration treatment”
e1“Mung bean (Vigna radiata)”
Claim · model-written summary
Caspase and total nuclease activity changed after dehydration.
- Taxon
- Mung bean (Vigna radiata)Vigna radiatae1
- Treatment
- dehydration treatmente0
- Gene
- caspase and total nucleasee0
- Outcome
- activity changes in caspase and total nucleasee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“activity changes in caspase and total nuclease in mung bean seeds after dehydration treatment”
e1“Mung bean (Vigna radiata)”
Claim · model-written summary
Loss of desiccation tolerance was related to programmed cell death.
- Taxon
- Mung bean (Vigna radiata)Vigna radiatae1
- Treatment
- dehydration treatmente2
- Outcome
- loss of desiccation tolerancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the loss of desiccation tolerance was related to PCD”
e1“Mung bean (Vigna radiata)”
e2“mung bean seeds after dehydration treatment”
Claim · model-written summary
Dehydration destroyed mitochondrial structure.
- Taxon
- Mung bean (Vigna radiata)Vigna radiatae1
- Treatment
- Dehydratione0
- Outcome
- mitochondrial structuree0
- Direction
- destructione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Dehydration resulted in the destruction of the mitochondrial structure”
e1“Mung bean (Vigna radiata)”
Claim · model-written summary
Dehydration reversed mitochondrial membrane potential.
- Taxon
- Mung bean (Vigna radiata)Vigna radiatae1
- Treatment
- Dehydratione0
- Outcome
- membrane potentiale0
- Direction
- reversale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Dehydration resulted in the destruction of the mitochondrial structure, reversal of the membrane potential”
e1“Mung bean (Vigna radiata)”
Claim · model-written summary
Dehydration caused cytochrome C to enter the cytoplasm.
- Taxon
- Mung bean (Vigna radiata)Vigna radiatae1
- Treatment
- Dehydratione0
- Gene
- cytochrome Ce0
- Outcome
- entrance of cytochrome C into the cytoplasme0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Dehydration resulted in the destruction of the mitochondrial structure, reversal of the membrane potential, and the entrance of cytochrome C into the cytoplasm”
e1“Mung bean (Vigna radiata)”
Claim · model-written summary
The authors identified the mitochondrial apoptosis pathway as the main form of dehydration-induced programmed cell death.
- Taxon
- Mung bean (Vigna radiata)Vigna radiatae1
- Outcome
- dehydration-induced PCDe0
- Qualifier
- mitochondrial apoptosis pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mitochondrial apoptosis pathway was the main form of dehydration-induced PCD”
e1“Mung bean (Vigna radiata)”
Claim · model-written summary
Ca2+ signaling played a role in inducing programmed cell death.
- Taxon
- Mung bean (Vigna radiata)Vigna radiatae1
- Compound
- Ca2+e0
- Outcome
- inducing PCDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ca2+ signaling also played a role in inducing PCD”
e1“Mung bean (Vigna radiata)”
Claim · model-written summary
The proposed pathway placed water potential upstream and ROS and mitochondrial PT channel downstream of Ca2+ signaling.
- Taxon
- Mung bean (Vigna radiata)Vigna radiatae1
- Compound
- Ca2+e2
- Outcome
- inducing PCDe2
- Qualifier
- upstream signal being dehydration-induced changes in water potential and the downstream signal being the ROS and mitochondrial PT channele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with the upstream signal being dehydration-induced changes in water potential and the downstream signal being the ROS and mitochondrial PT channel”
e1“Mung bean (Vigna radiata)”
e2“Ca2+ signaling also played a role in inducing PCD”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.