Abstract
Puccinia striiformis f. sp. tritici causes wheat stripe rust, one of most important diseases of wheat worldwide. High-temperature seedling plant (HTSP) resistance of wheat to P. striiformis f. sp. tritici is one specific type of host resistance, induced by high temperature (HT). Receptor-like kinases (RLKs) play key roles in regulating plant development and signaling networks, but there have been no reports on possible roles played by RLKs in wheat HTSP to P. striiformis f. sp. tritici. In the present study, a leucine rich repeat (LRR)-RLK gene, TaXa21, with a high homology with rice bacterial blight resistance gene Xa21, was cloned from wheat cultivar Xiaoyan 6 (XY 6). TaXa21 expression was up-regulated by the exposure to HT (20°C) for 24 h at 8 days postinoculation with P. striiformis f. sp. tritici and was induced by ethylene (ET) and hydrogen peroxide (H 2 O 2 ). Knocking down TaXa21 using virus-induced gene silencing reduced HTSP resistance to P. striiformis f. sp. tritici compared with the control plants. In addition, the expression level of TaCAT in the H 2 O 2 pathway was induced and TaACO in the ET signal pathway was reduced in the HT-treated TaXa21-silenced plants. Transient expression of TaXa21 in tobacco leaves confirmed its subcellular localization in plasma membrane, consistent with the prediction from bioinformatics analysis. The transmembrane and kinase domain of TaXa21 can interact with TaWRKY76 in the nucleus and cell membrane, which is different from the localization of Xa21 in rice. The interaction between TaWRKY76 and TaWRKY62 (positively involved in the HTSP resistance of XY 6) were observed. Together, these results indicated that TaXa21 is a RLK associated with TaWRKY76 and TaWRKY62 and functions as a positive regulator of wheat HTSP resistance to P. striiformis f. sp. tritici. Furthermore, the host defense is mediated by the H 2 O 2 and ET signal pathways.
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Claims 12
Claim · model-written summary
TaXa21 was cloned from wheat cultivar Xiaoyan 6.
- Taxon
- wheat
Triticum aestivume0 - Material
- Xiaoyan 6e0
- Gene
- TaXa21e0
- Outcome
- was clonede0
- Comparator
- rice bacterial blight resistance gene Xa21e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a leucine rich repeat (LRR)-RLK gene, TaXa21, with a high homology with rice bacterial blight resistance gene Xa21, was cloned from wheat cultivar Xiaoyan 6 (XY 6)”
Claim · model-written summary
High temperature up-regulated TaXa21 expression after stripe rust inoculation.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- exposure to HT (20°C) for 24 he0
- Interactor
- P. striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie0pathogen - Gene
- TaXa21e0
- Outcome
- TaXa21 expressione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaXa21 expression was up-regulated by the exposure to HT (20°C) for 24 h at 8 days postinoculation with P. striiformis f. sp. tritici”
t0“Wheat High-Temperature Seedling Plant Resistance”
Claim · model-written summary
Ethylene induced TaXa21 expression.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- ethylene (ET)e0
- Outcome
- was inducede0
- Direction
- inducede0
Not reportedGeneComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was induced by ethylene (ET) and hydrogen peroxide (H 2 O 2 )”
t0“Wheat High-Temperature Seedling Plant Resistance”
Claim · model-written summary
Hydrogen peroxide induced TaXa21 expression.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- hydrogen peroxide (H 2 O 2 )e0
- Outcome
- was inducede0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was induced by ethylene (ET) and hydrogen peroxide (H 2 O 2 )”
t0“Wheat High-Temperature Seedling Plant Resistance”
Claim · model-written summary
Silencing TaXa21 reduced wheat high-temperature seedling resistance to stripe rust.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- Knocking down TaXa21 using virus-induced gene silencinge0
- Interactor
- P. striiformis f. sp. triticiPuccinia striiformis f. sp. triticie0pathogen
- Gene
- TaXa21e0
- Outcome
- HTSP resistance to P. striiformis f. sp. triticie0
- Direction
- reducede0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knocking down TaXa21 using virus-induced gene silencing reduced HTSP resistance to P. striiformis f. sp. tritici compared with the control plants”
t0“Wheat High-Temperature Seedling Plant Resistance”
Claim · model-written summary
HT-treated TaXa21-silenced plants showed induced TaCAT expression.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaCATe0
- Outcome
- expression level of TaCATe0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression level of TaCAT in the H 2 O 2 pathway was induced”
t0“Wheat High-Temperature Seedling Plant Resistance”
Claim · model-written summary
HT-treated TaXa21-silenced plants showed reduced TaACO expression.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- HT-treated TaXa21-silencede0
- Gene
- TaACOe0
- Outcome
- TaACO in the ET signal pathwaye0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaACO in the ET signal pathway was reduced in the HT-treated TaXa21-silenced plants”
t0“Wheat High-Temperature Seedling Plant Resistance”
Claim · model-written summary
Transient expression localized TaXa21 to the plasma membrane of tobacco leaves.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- leavese0
- Gene
- TaXa21e0
- Outcome
- subcellular localization in plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of TaXa21 in tobacco leaves confirmed its subcellular localization in plasma membrane”
Claim · model-written summary
TaXa21 transmembrane and kinase domains interacted with TaWRKY76 in the nucleus and cell membrane.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaXa21e0
- Outcome
- interact with TaWRKY76 in the nucleus and cell membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transmembrane and kinase domain of TaXa21 can interact with TaWRKY76 in the nucleus and cell membrane”
t0“Wheat High-Temperature Seedling Plant Resistance”
Claim · model-written summary
TaWRKY76 and TaWRKY62 interacted.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaWRKY76e0
- Outcome
- interaction between TaWRKY76 and TaWRKY62e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between TaWRKY76 and TaWRKY62 (positively involved in the HTSP resistance of XY 6) were observed”
t0“Wheat High-Temperature Seedling Plant Resistance”
Claim · model-written summary
TaXa21 was interpreted as a positive regulator of wheat high-temperature seedling resistance to stripe rust.
- Taxon
- wheatTriticum aestivume0
- Interactor
- P. striiformis f. sp. triticiPuccinia striiformis f. sp. triticie0pathogen
- Gene
- TaXa21e0
- Outcome
- wheat HTSP resistance to P. striiformis f. sp. triticie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaXa21 is a RLK associated with TaWRKY76 and TaWRKY62 and functions as a positive regulator of wheat HTSP resistance to P. striiformis f. sp. tritici”
Claim · model-written summary
The authors inferred mediation of wheat host defense by hydrogen peroxide and ethylene pathways.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- host defensee0
- Qualifier
- H 2 O 2 and ET signal pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the host defense is mediated by the H 2 O 2 and ET signal pathways”
t0“Wheat High-Temperature Seedling Plant Resistance”
The same questions in other papers
loss of function · Puccinia striiformis f. sp. tritici · Triticum aestivum (wheat)Positive and negative results
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- positive
- positive
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.