Abstract
Biodiversity in plant shape is mainly attributable to the diversity of leaf shape, which is largely determined by the transient morphogenetic activity of the leaf margin that creates leaf serrations. However, the precise mechanism underlying the establishment of this morphogenetic capacity remains poorly understood. We report here that INDOLE-3-BUTYRIC ACID RESPONSE 5 (IBR5), a dual-specificity phosphatase, is a key component of leaf-serration regulatory machinery. Loss-of-function mutants of IBR5 exhibited pronounced serrations due to increased cell area. IBR5 was localized in the nucleus of leaf epidermis and petiole cells. Introducing a C129S mutation within the highly conserved VxVHCx2GxSRSx5AYLM motif of IBR5 rendered it unable to rescue the leaf-serration defects of the ibr5-3 mutant. In addition, auxin reporters revealed that the distribution of auxin maxima was expanded ectopically in ibr5-3. Furthermore, we found that the distribution of PIN1 on the plasma membrane of the epidermal and cells around the leaf vein was compromised in ibr5-3. We concluded that IBR5 is essential for the establishment of PIN-FORMED 1 (PIN1)-directed auxin maxima at the tips of leaf serration, which is vital for the elaborated regulation during its formation.
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Claims 6
Claim · model-written summary
IBR5 loss-of-function mutants showed pronounced serrations associated with increased cell area.
- Material
- Loss-of-function mutants of IBR5e0
- Gene
- IBR5e0
- Outcome
- pronounced serrationse0
- Qualifier
- increased cell areae0
Not reportedTaxon
Verbatim
e0“Loss-of-function mutants of IBR5 exhibited pronounced serrations due to increased cell area.”
Claim · model-written summary
IBR5 localized in nuclei of leaf epidermis and petiole cells.
Not reportedTaxon
Verbatim
e0“IBR5 was localized in the nucleus of leaf epidermis and petiole cells.”
Claim · model-written summary
The C129S mutation prevented rescue of leaf serration defects in ibr5-3.
- Material
- ibr5-3 mutante0
- Treatment
- Introducing a C129S mutatione0
- Gene
- IBR5e0
- Outcome
- leaf-serration defectse0
- Direction
- unable to rescuee0
Not reportedTaxonComparator
Verbatim
e0“Introducing a C129S mutation within the highly conserved VxVHCx2GxSRSx5AYLM motif of IBR5 rendered it unable to rescue the leaf-serration defects of the ibr5-3 mutant.”
Claim · model-written summary
Auxin maxima distribution expanded ectopically in ibr5-3.
Not reportedTaxon
Verbatim
e0“the distribution of auxin maxima was expanded ectopically in ibr5-3.”
Claim · model-written summary
PIN1 distribution on the plasma membrane was compromised in ibr5-3.
- Material
- ibr5-3e0
- Organ / stage
- leaf veine0
- Gene
- PIN1e0
- Outcome
- distribution of PIN1e0
- Direction
- compromisede0
Not reportedTaxon
Verbatim
e0“the distribution of PIN1 on the plasma membrane of the epidermal and cells around the leaf vein was compromised in ibr5-3.”
Claim · model-written summary
The authors conclude IBR5 is essential to establish PIN1-directed auxin maxima at leaf serration tips.
- Organ / stage
- leaf serratione0
- Gene
- IBR5e0
- Outcome
- establishment of PIN-FORMED 1 (PIN1)-directed auxin maximae0
Not reportedTaxon
Verbatim
e0“IBR5 is essential for the establishment of PIN-FORMED 1 (PIN1)-directed auxin maxima at the tips of leaf serration”