Abstract
Abstract A short period (240°C hr; Tb = 25°C) of heat stress (30°C day/25°C night) during forage rape ( Brassica napus L.) seed development or at seed physiological maturity can reduce seed vigour, but the extent of oxidative damage associated with this short heat stress was not known. Heat‐stressed seeds were assessed for malondialdehyde (MDA) content, hydrogen peroxide (H 2 O 2 ) accumulation, antioxidant enzyme activity, adenylate energy charge and seed ultrastructural integrity, and compared with that of non‐heat‐stressed seeds. Heat stress increased both MDA content and H 2 O 2 accumulation by 35%–50%, reduced antioxidant enzyme activity by between 12% and 67%, and significantly reduced adenosine energy charge. Transmission electron microscope images showed clear evidence of seed deterioration in heat‐stressed seeds, including ruptures in cell wall and plasma membranes, fused lipid bodies and damaged mitochondria. Heat stress at physiological maturity caused more oxidative damage than the same heat stress during seed development. Seed vigour decreased as H 2 O 2 accumulation increased and antioxidant enzyme activity decreased, but no direct relationship between lipid peroxidation and seed vigour was established. The extent of damage resulting from even shorter periods of heat stress (<240°C hr) before or at seed physiological maturity requires investigation.
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Claims 8
Claim · model-written summary
Heat stress increased MDA content and H2O2 accumulation by 35%–50% compared with non-heat-stressed seeds.
- Taxon
- Brassica napus L.
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Treatment
- Heat stresse1
- Outcome
- MDA content and H 2 O 2 accumulatione1
- Direction
- increasede1
- Value
- 35%–50%e1
- Comparator
- non‐heat‐stressed seedse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“forage rape ( Brassica napus L.) seed development”
e1“Heat stress increased both MDA content and H 2 O 2 accumulation by 35%–50%, reduced antioxidant enzyme activity by between 12% and 67%, and significantly reduced adenosine energy charge”
e2“compared with that of non‐heat‐stressed seeds”
Claim · model-written summary
Heat stress reduced antioxidant enzyme activity by 12% to 67% relative to non-heat-stressed seeds.
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- Heat stresse1
- Outcome
- antioxidant enzyme activitye1
- Direction
- reducede1
- Value
- 12% and 67%e1
- Comparator
- non‐heat‐stressed seedse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“forage rape ( Brassica napus L.) seed development”
e1“Heat stress increased both MDA content and H 2 O 2 accumulation by 35%–50%, reduced antioxidant enzyme activity by between 12% and 67%, and significantly reduced adenosine energy charge”
e2“compared with that of non‐heat‐stressed seeds”
Claim · model-written summary
Heat stress significantly reduced adenosine energy charge relative to non-heat-stressed seeds.
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- Heat stresse1
- Outcome
- adenosine energy chargee1
- Direction
- reducede1
- Comparator
- non‐heat‐stressed seedse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“forage rape ( Brassica napus L.) seed development”
e1“Heat stress increased both MDA content and H 2 O 2 accumulation by 35%–50%, reduced antioxidant enzyme activity by between 12% and 67%, and significantly reduced adenosine energy charge”
e2“compared with that of non‐heat‐stressed seeds”
Claim · model-written summary
Transmission electron microscopy showed seed deterioration in heat-stressed seeds, including ruptured membranes, fused lipid bodies and damaged mitochondria.
- Taxon
- Brassica napus L.Brassica napuse0
- Outcome
- seed deterioratione3
- Qualifier
- ruptures in cell wall and plasma membranes, fused lipid bodies and damaged mitochondriae3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“forage rape ( Brassica napus L.) seed development”
e3“Transmission electron microscope images showed clear evidence of seed deterioration in heat‐stressed seeds, including ruptures in cell wall and plasma membranes, fused lipid bodies and damaged mitochondria”
Claim · model-written summary
Heat stress at physiological maturity caused more oxidative damage than the same heat stress during seed development.
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- Heat stress at physiological maturitye4
- Outcome
- oxidative damagee4
- Direction
- moree4
- Comparator
- the same heat stress during seed developmente4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“forage rape ( Brassica napus L.) seed development”
e4“Heat stress at physiological maturity caused more oxidative damage than the same heat stress during seed development”
Claim · model-written summary
Seed vigour decreased as H2O2 accumulation increased.
- Taxon
- Brassica napus L.Brassica napuse0
- Compound
- H 2 O 2e5
- Outcome
- Seed vigoure5
- Direction
- decreasede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“forage rape ( Brassica napus L.) seed development”
e5“Seed vigour decreased as H 2 O 2 accumulation increased and antioxidant enzyme activity decreased”
Claim · model-written summary
Seed vigour decreased as antioxidant enzyme activity decreased.
- Taxon
- Brassica napus L.Brassica napuse0
- Gene
- antioxidant enzymee5
- Outcome
- Seed vigoure5
- Direction
- decreasede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“forage rape ( Brassica napus L.) seed development”
e5“Seed vigour decreased as H 2 O 2 accumulation increased and antioxidant enzyme activity decreased”
Claim · model-written summary
No direct relationship between lipid peroxidation and seed vigour was established.
- Taxon
- Brassica napus L.Brassica napuse0
- Outcome
- relationship between lipid peroxidation and seed vigoure6
- Qualifier
- no direct relationshipe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“forage rape ( Brassica napus L.) seed development”
e6“no direct relationship between lipid peroxidation and seed vigour was established”
The same questions in other papers
heat · molecular: metabolites · Brassica napus (oilseed rape)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
1 paper more in this group
See all 5 papers →heat · physiology: oxidative · Brassica napus (oilseed rape)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.