Paper · 2019 · Annual Research & Review in Biology

Histological Studies of Cassava (Manihot esculenta Crantz) Root Infection Using Aspergillus niger Rhizopus stolonifer, Aspergillus niger, Aspergillus flavus, Botryodiplodia theobromae and Trichoderma viride

A. C. Amadioha; Kenkwo Promise Chidi

5
claims
8
verbatim quotes
0
citations
1
research areas
✓
Open access

Abstract

Histological studies were carried out to investigate the impact of some pathogenic organisms on infected cassava root after different periods of incubation. Cassava roots inoculated with Aspergillus niger, Rhizopus stolonifer, Aspergillus flavus, and Trichoderma viride showed progressive depletion of the starch grains in the cells and tissue maceration of the cassava root as the period of incubation increased. Cassava root inoculated with A. flavus showed tissue necrosis and slight fragmentation of the starch grains whereas, cassava tissues infected with A. niger showed tissue necrosis and general maceration of the starch grains within the tissue thereby creating empty spaces within the cells with no clear arrangement of the starch grains. Inoculation of the cassava tissues with R. stolonifer resulted in rapid loss of the starch grains and erosion of surrounding cell boundaries. Cassava tissue colonized by T. viride showed tissue necrosis and maceration of the starch grains. Healthy (uninoculated) cassava root tissue showed intact cells packed with large starch grains suggesting that in the host-parasite relationship, the pathogens established themselves inter and intracellularly in the parenchymatous cells of the cortex of the cassava root clearing them of starch grains, and resulting to poor yield and nutritional value of cassava and also economic loss.

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Claims 5

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

A. flavus inoculation produced tissue necrosis and slight starch grain fragmentation in cassava roots.

Treatment
inoculated with A. flavuse0
Interactor
A. flavusAspergillus flavuse0pathogen
Outcome
tissue necrosis and slight fragmentation of the starch grainse0
Direction
showede0
Comparator
uninoculatede1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗
Aspergillus flavus
Aspergillus flavus · InteractorUS gov · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Cassava root inoculated with A. flavus showed tissue necrosis and slight fragmentation of the starch grains”

✓ located verbatim in the source · abstract, characters 414–521 · show in text ↑
e1

“Healthy (uninoculated) cassava root tissue showed intact cells packed with large starch grains”

✓ located verbatim in the source · abstract, characters 990–1084 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

A. niger infection produced necrosis and extensive starch grain maceration.

Treatment
infected with A. nigere0
Interactor
A. nigerAspergillus nigere0pathogen
Outcome
tissue necrosis and general maceration of the starch grainse0
Direction
showede0
Comparator
uninoculatede1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗
Aspergillus niger
Aspergillus niger · InteractorNo machine-readable author provided. Y tambe assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“cassava tissues infected with A. niger showed tissue necrosis and general maceration of the starch grains within the tissue thereby creating empty spaces within the cells”

✓ located verbatim in the source · abstract, characters 531–701 · show in text ↑
e1

“Healthy (uninoculated) cassava root tissue showed intact cells packed with large starch grains”

✓ located verbatim in the source · abstract, characters 990–1084 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

R. stolonifer inoculation caused rapid starch grain loss and erosion of cell boundaries.

Treatment
Inoculation of the cassava tissues with R. stolonifere0
Interactor
R. stoloniferRhizopus stolonifere0pathogen
Outcome
loss of the starch grains and erosion of surrounding cell boundariese0
Direction
rapid losse0
Comparator
uninoculatede1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗
Rhizopus stolonifer
Rhizopus stolonifer · InteractorW.A. Djatmiko ( Wie146 ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Inoculation of the cassava tissues with R. stolonifer resulted in rapid loss of the starch grains and erosion of surrounding cell boundaries”

✓ located verbatim in the source · abstract, characters 750–890 · show in text ↑
e1

“Healthy (uninoculated) cassava root tissue showed intact cells packed with large starch grains”

✓ located verbatim in the source · abstract, characters 990–1084 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

T. viride colonization was associated with tissue necrosis and starch grain maceration.

Interactor
T. virideTrichoderma viridee0pathogen
Outcome
tissue necrosis and maceration of the starch grainse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗
Trichoderma viride
Trichoderma viride · InteractorKatja Schulz · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Cassava tissue colonized by T. viride showed tissue necrosis and maceration of the starch grains”

✓ located verbatim in the source · abstract, characters 892–988 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Healthy uninoculated cassava roots had intact cells packed with large starch grains.

Outcome
intact cells packed with large starch grainse0
Qualifier
uninoculatede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“Healthy (uninoculated) cassava root tissue showed intact cells packed with large starch grains”

✓ located verbatim in the source · abstract, characters 990–1084 · show in text ↑

inoculation · quality: sugar starch · Manihot esculenta (cassava)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
See all 3 papers →

infection · disease · Manihot esculenta (cassava)Positive and negative results

5 papers: 1 positive, 4 negative or no effect

3 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. no effect
  3. no effect

1 paper more in this group

See all 5 papers →

inoculation · disease · Manihot esculenta (cassava)Positive and negative results

19 papers: 9 positive, 10 negative or no effect

9 papers in the same direction as this paper · 9 in the opposite direction

  1. positive
  2. positive
  3. positive

15 papers more in this group

See all 19 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.