Abstract
The exploration of relationship between orchid and its endophytic fungi is significant as vast arrays of beneficial attributes are provided by the fungi to its host which includes the production of phytohormones like Indole acetic acid (IAA). The present investigation focused on production, characterization and applications of IAA from endophytic fungi of Cymbidium aloifolium, an epiphytic orchid. The Colletotrichum gloeosporioides (CAR4) isolated and characterized from C. aloifolium produced higher Indole acetic acid (10.7±1.97 ìM/mL). The CAR4 IAA produced was confirmed by UV/Vis spectrum, TLC, FTIR, HPLC, LCMS, NMR analysis. The CAR4 IAA treated Macrotyloma uniflorum and Vigna unguiculata seeds exhibited increased germination percentage by 3 - 8 %, root length by 1 - 14 mm, shoot length by 2 - 21 mm compared to standard IAA. The Murashige and Skoog’s Basal media (MSBM) with coconut milk (10 %), casein hydrolysate (250 mg/L) showed swelling of C. aloifolium seeds within 15 days and well developed protocorms after 60 days of incubation. The differentiation of protocorms into roots and shoots was higher in MSBM with CAR4 IAA (0.5 mg/L) and BAP (6-Benzylaminopurine) (1 mg/L) with average of 8.5 ± 0.83 shoots and 5.67 ± 0.81 roots. The culture parameters were optimized and maximum IAA production was in PDB with 4 mg/mL of ⟨-tryptophan, pH6, 30°C, 15 days incubation in dark. IAA production increased four folds with optimized parameters.
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Claims 9
Claim · model-written summary
Colletotrichum gloeosporioides (CAR4) from C. aloifolium produced 10.7±1.97 µM/mL of indole acetic acid.
- Taxon
- Cymbidium aloifolium
Cymbidium aloifoliumt0 - Material
- CAR4e0
- Interactor
- Colletotrichum gloeosporioides
Colletotrichum gloeosporioidese0symbiont - Outcome
- Indole acetic acide0
- Value
- 10.7±1.97 ìM/mLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Production, Characterization of Indole Acetic Acid and its Bioactive Potential from Endophytic Fungi ofCymbidium aloifoliumL.”
e0“The Colletotrichum gloeosporioides (CAR4) isolated and characterized from C. aloifolium produced higher Indole acetic acid (10.7±1.97 ìM/mL)”
Claim · model-written summary
CAR4 IAA increased germination percentage of Macrotyloma uniflorum and Vigna unguiculata seeds by 3 to 8% compared with standard IAA.
- Taxon
- Macrotyloma uniflorum and Vigna unguiculata
Vigna unguiculata
Macrotyloma uniflorume1 - Treatment
- CAR4 IAAe1
- Outcome
- germination percentagee1
- Direction
- increasede1
- Value
- 3 - 8 %e1
- Comparator
- standard IAAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Production, Characterization of Indole Acetic Acid and its Bioactive Potential from Endophytic Fungi ofCymbidium aloifoliumL.”
e1“The CAR4 IAA treated Macrotyloma uniflorum and Vigna unguiculata seeds exhibited increased germination percentage by 3 - 8 %, root length by 1 - 14 mm, shoot length by 2 - 21 mm compared to standard IAA”
Claim · model-written summary
CAR4 IAA increased root length of Macrotyloma uniflorum and Vigna unguiculata seedlings by 1 to 14 mm compared with standard IAA.
- Taxon
- Macrotyloma uniflorum and Vigna unguiculataVigna unguiculataMacrotyloma uniflorume1
- Treatment
- CAR4 IAAe1
- Outcome
- root lengthe1
- Direction
- increasede1
- Value
- 1 - 14 mme1
- Comparator
- standard IAAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Production, Characterization of Indole Acetic Acid and its Bioactive Potential from Endophytic Fungi ofCymbidium aloifoliumL.”
e1“The CAR4 IAA treated Macrotyloma uniflorum and Vigna unguiculata seeds exhibited increased germination percentage by 3 - 8 %, root length by 1 - 14 mm, shoot length by 2 - 21 mm compared to standard IAA”
Claim · model-written summary
CAR4 IAA increased shoot length of Macrotyloma uniflorum and Vigna unguiculata seedlings by 2 to 21 mm compared with standard IAA.
- Taxon
- Macrotyloma uniflorum and Vigna unguiculataVigna unguiculataMacrotyloma uniflorume1
- Treatment
- CAR4 IAAe1
- Outcome
- shoot lengthe1
- Direction
- increasede1
- Value
- 2 - 21 mme1
- Comparator
- standard IAAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Production, Characterization of Indole Acetic Acid and its Bioactive Potential from Endophytic Fungi ofCymbidium aloifoliumL.”
e1“The CAR4 IAA treated Macrotyloma uniflorum and Vigna unguiculata seeds exhibited increased germination percentage by 3 - 8 %, root length by 1 - 14 mm, shoot length by 2 - 21 mm compared to standard IAA”
Claim · model-written summary
Protocorm differentiation into roots and shoots was higher with CAR4 IAA plus BAP, at 8.5 shoots and 5.67 roots on average.
- Taxon
- Cymbidium aloifoliumCymbidium aloifoliumt0
- Organ / stage
- protocormse2
- Treatment
- CAR4 IAA (0.5 mg/L) and BAP (6-Benzylaminopurine) (1 mg/L)e2
- Outcome
- differentiation of protocorms into roots and shootse2
- Direction
- highere2
- Value
- 8.5 ± 0.83 shoots and 5.67 ± 0.81 rootse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Production, Characterization of Indole Acetic Acid and its Bioactive Potential from Endophytic Fungi ofCymbidium aloifoliumL.”
e2“The differentiation of protocorms into roots and shoots was higher in MSBM with CAR4 IAA (0.5 mg/L) and BAP (6-Benzylaminopurine) (1 mg/L) with average of 8.5 ± 0.83 shoots and 5.67 ± 0.81 roots”
Claim · model-written summary
Seeds of C. aloifolium swelled within 15 days on MSBM with coconut milk and casein hydrolysate.
- Taxon
- Cymbidium aloifoliumCymbidium aloifoliumt0
- Outcome
- swelling of C. aloifolium seedse3
- Qualifier
- within 15 dayse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Production, Characterization of Indole Acetic Acid and its Bioactive Potential from Endophytic Fungi ofCymbidium aloifoliumL.”
e3“The Murashige and Skoog’s Basal media (MSBM) with coconut milk (10 %), casein hydrolysate (250 mg/L) showed swelling of C. aloifolium seeds within 15 days and well developed protocorms after 60 days of incubation”
Claim · model-written summary
Well developed protocorms formed on MSBM after 60 days of incubation.
- Taxon
- Cymbidium aloifoliumCymbidium aloifoliumt0
- Outcome
- well developed protocormse3
- Qualifier
- after 60 days of incubatione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Production, Characterization of Indole Acetic Acid and its Bioactive Potential from Endophytic Fungi ofCymbidium aloifoliumL.”
e3“The Murashige and Skoog’s Basal media (MSBM) with coconut milk (10 %), casein hydrolysate (250 mg/L) showed swelling of C. aloifolium seeds within 15 days and well developed protocorms after 60 days of incubation”
Claim · model-written summary
Maximum IAA production was obtained in PDB with 4 mg/mL tryptophan, pH 6, 30 °C and 15 days incubation in the dark.
- Taxon
- Cymbidium aloifoliumCymbidium aloifoliumt0
- Outcome
- maximum IAA productione4
- Qualifier
- PDB with 4 mg/mL of ⟨-tryptophan, pH6, 30°C, 15 days incubation in darke4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Production, Characterization of Indole Acetic Acid and its Bioactive Potential from Endophytic Fungi ofCymbidium aloifoliumL.”
e4“The culture parameters were optimized and maximum IAA production was in PDB with 4 mg/mL of ⟨-tryptophan, pH6, 30°C, 15 days incubation in dark”
Claim · model-written summary
IAA production increased four-fold with optimized culture parameters.
- Taxon
- Cymbidium aloifoliumCymbidium aloifoliumt0
- Treatment
- optimized parameterse5
- Outcome
- IAA productione5
- Direction
- increasede5
- Value
- four foldse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Production, Characterization of Indole Acetic Acid and its Bioactive Potential from Endophytic Fungi ofCymbidium aloifoliumL.”
e5“IAA production increased four folds with optimized parameters”
The same questions in other papers
indole-3-acetic acid · growth · Vigna unguiculata (cowpea)Positive and negative results
7 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.