Abstract
Abstract Nutritional and hormonal requirements for in vitro growth of callus tissue of Vigna sinensis Endl. were studied. Callus was formed on hypocotyl and root sections, when they were cultured on Linsmaier and Skoog's basal medium solidified with 10 g/1 agar and supplemented with only 0.5 mg/12,4‐D or 2 mg/1 IAA. Further addition of 0.2 mg/1 kinetin and 1 g/l yeast extract resulted in more active callus formation. For unlimited vigorous growth of subcultured callus which was originally isolated from root sections, yeast extract was indispensable besides 2,4‐D and kinetin. Such growth‐promoting activity was observed also in malt extract and Ebios (dried cell powder of brewery yeast). Of known compounds tested, nicotinic acid, nicotinamide., methyl nicotinate and NAD were promotive to the growth of the callus, although much less effective than yeast extract. Other pyridine derivatives, vitamins and amino acids tested were ineffective or slightly effective. Sucrose was the most suitable carbon source. Fructose, glucose and maltose also supported the growth. Kinetin stimulated cell proliferation of the callus and cell differentiation to tracheary element.
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Claims 12
Claim · model-written summary
Callus formed on hypocotyl and root sections with 2,4-D or IAA.
- Taxon
- Vigna sinensis
Vigna unguiculata subsp. unguiculatat0 - Organ / stage
- hypocotyl and root sectionse0
- Treatment
- 0.5 mg/12,4‐D or 2 mg/1 IAAe0
- Outcome
- Callus was formede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus was formed on hypocotyl and root sections, when they were cultured on Linsmaier and Skoog's basal medium solidified with 10 g/1 agar and supplemented with only 0.5 mg/12,4‐D or 2 mg/1 IAA.”
t0“Nutritional and Hormonal Requirements for the Growth of Vigna sinensis Callus in vitro”
Claim · model-written summary
Kinetin and yeast extract promoted more active callus formation.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- 0.2 mg/1 kinetin and 1 g/l yeast extracte0
- Outcome
- callus formatione0
- Direction
- more activee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Further addition of 0.2 mg/1 kinetin and 1 g/l yeast extract resulted in more active callus formation.”
t0“Nutritional and Hormonal Requirements for the Growth of Vigna sinensis Callus in vitro”
Claim · model-written summary
Yeast extract was indispensable for unlimited vigorous subcultured callus growth.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Organ / stage
- subcultured calluse0
- Treatment
- yeast extracte0
- Outcome
- unlimited vigorous growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For unlimited vigorous growth of subcultured callus which was originally isolated from root sections, yeast extract was indispensable besides 2,4‐D and kinetin.”
t0“Nutritional and Hormonal Requirements for the Growth of Vigna sinensis Callus in vitro”
Claim · model-written summary
Malt extract also promoted callus growth.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- malt extracte0
- Outcome
- growth‐promoting activitye0
- Direction
- growth‐promotinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Such growth‐promoting activity was observed also in malt extract and Ebios (dried cell powder of brewery yeast).”
t0“Nutritional and Hormonal Requirements for the Growth of Vigna sinensis Callus in vitro”
Claim · model-written summary
Ebios also promoted callus growth.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- Ebiose0
- Outcome
- growth‐promoting activitye0
- Direction
- growth‐promotinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Such growth‐promoting activity was observed also in malt extract and Ebios (dried cell powder of brewery yeast).”
t0“Nutritional and Hormonal Requirements for the Growth of Vigna sinensis Callus in vitro”
Claim · model-written summary
Nicotinic acid, nicotinamide and methyl nicotinate promoted callus growth less than yeast extract.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- nicotinic acid, nicotinamide., methyl nicotinatee0
- Outcome
- growth of the calluse0
- Direction
- much less effectivee0
- Comparator
- yeast extracte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nicotinic acid, nicotinamide., methyl nicotinate and NAD were promotive to the growth of the callus, although much less effective than yeast extract.”
t0“Nutritional and Hormonal Requirements for the Growth of Vigna sinensis Callus in vitro”
Claim · model-written summary
NAD promoted callus growth less than yeast extract.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- NADe0
- Outcome
- growth of the calluse0
- Direction
- much less effectivee0
- Comparator
- yeast extracte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAD were promotive to the growth of the callus, although much less effective than yeast extract.”
t0“Nutritional and Hormonal Requirements for the Growth of Vigna sinensis Callus in vitro”
Claim · model-written summary
Other tested pyridine derivatives, vitamins and amino acids were ineffective or slightly effective.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- Other pyridine derivatives, vitamins and amino acidse0
- Outcome
- ineffective or slightly effectivee0
- Direction
- ineffective or slightly effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Other pyridine derivatives, vitamins and amino acids tested were ineffective or slightly effective.”
t0“Nutritional and Hormonal Requirements for the Growth of Vigna sinensis Callus in vitro”
Claim · model-written summary
Sucrose was the most suitable carbon source.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- Sucrosee0
- Outcome
- most suitable carbon sourcee0
- Direction
- most suitablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose was the most suitable carbon source.”
t0“Nutritional and Hormonal Requirements for the Growth of Vigna sinensis Callus in vitro”
Claim · model-written summary
Fructose, glucose and maltose supported growth.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- Fructose, glucose and maltosee0
- Outcome
- growthe0
- Direction
- supportede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fructose, glucose and maltose also supported the growth.”
t0“Nutritional and Hormonal Requirements for the Growth of Vigna sinensis Callus in vitro”
Claim · model-written summary
Kinetin stimulated callus cell proliferation.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- Kinetine0
- Outcome
- cell proliferation of the calluse0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kinetin stimulated cell proliferation of the callus and cell differentiation to tracheary element.”
t0“Nutritional and Hormonal Requirements for the Growth of Vigna sinensis Callus in vitro”
Claim · model-written summary
Kinetin stimulated differentiation into tracheary elements.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- Kinetine0
- Outcome
- cell differentiation to tracheary elemente0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kinetin stimulated cell proliferation of the callus and cell differentiation to tracheary element.”
t0“Nutritional and Hormonal Requirements for the Growth of Vigna sinensis Callus in vitro”
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