Paper · 1983 · Institutional Repositories DataBase (IRDB)

Hormonal Regulation of Cell Proliferation and Differentiation of Vigna sinensis Callus in Vitro (A. NATURAL SCIENCE)

Satoshi Matsubara

10
claims
19
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

The growth of Vigna sinensis callus was promoted synergistically by auxin, cytokinin and nicotinic acid. Strong auxins such as 2,4,5-T (2,4,5-trichlorophenoxyacetic acid), 2,4-D (2,4-dichlorophenoxyacetic acid) and NAA (α-naphthaleneacetic acid) at low concentration (1 or 0.1μM) stimulated cell proliferation, but inhibited root formation. High concentration (22.1μM) of 2,4-D caused watery friable callus composed of free round vacuolated and undifferentiated cells. On the other hand, an addition of kinetin decreased free cell formation and gave compact tissue. High level (10 and 40μM) of kinetin induced cell differentiation to tracheal element, but 40μM kinetin rather inhibited formation of tracheal element in the case of low level (0 or 0.0221μM) of 2,4-D. Thus, relative amounts of auxins and cytokinins determined cell characteristics and differentiation. Nicotinic acid had no effect on tissue texture, cell characteristics and differentiation, although stimulated strongly cell proliferation.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Auxin, cytokinin and nicotinic acid synergistically promoted callus growth.

Treatment
auxin, cytokinin and nicotinic acide0
Outcome
growth of Vigna sinensis calluse0
Direction
promoted synergisticallye0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata subsp. unguiculata
Vigna unguiculata subsp. unguiculata · TaxonWibowo Djatmiko ( Wie146 ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The growth of Vigna sinensis callus was promoted synergistically by auxin, cytokinin and nicotinic acid.”

✓ located verbatim in the source · abstract, characters 0–104 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Low concentrations of strong auxins stimulated cell proliferation.

Treatment
Strong auxins such as 2,4,5-T (2,4,5-trichlorophenoxyacetic acid), 2,4-D (2,4-dichlorophenoxyacetic acid) and NAA (α-naphthaleneacetic acid) at low concentration (1 or 0.1μM)e0
Outcome
cell proliferatione0
Direction
stimulatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata subsp. unguiculata
Vigna unguiculata subsp. unguiculata · TaxonWibowo Djatmiko ( Wie146 ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Strong auxins such as 2,4,5-T (2,4,5-trichlorophenoxyacetic acid), 2,4-D (2,4-dichlorophenoxyacetic acid) and NAA (α-naphthaleneacetic acid) at low concentration (1 or 0.1μM) stimulated cell proliferation”

✓ located verbatim in the source · abstract, characters 105–309 · show in text ↑
t0

“Vigna sinensis”

✓ located verbatim in the source · title, characters 65–79 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Low concentrations of strong auxins inhibited root formation.

Treatment
Strong auxins such as 2,4,5-T (2,4,5-trichlorophenoxyacetic acid), 2,4-D (2,4-dichlorophenoxyacetic acid) and NAA (α-naphthaleneacetic acid) at low concentration (1 or 0.1μM)e0
Outcome
root formatione0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata subsp. unguiculata
Vigna unguiculata subsp. unguiculata · TaxonWibowo Djatmiko ( Wie146 ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Strong auxins such as 2,4,5-T (2,4,5-trichlorophenoxyacetic acid), 2,4-D (2,4-dichlorophenoxyacetic acid) and NAA (α-naphthaleneacetic acid) at low concentration (1 or 0.1μM) stimulated cell proliferation, but inhibited root formation.”

✓ located verbatim in the source · abstract, characters 105–340 · show in text ↑
t0

“Vigna sinensis”

✓ located verbatim in the source · title, characters 65–79 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

High 2,4-D concentration produced watery friable undifferentiated callus.

Treatment
High concentration (22.1μM) of 2,4-De0
Outcome
watery friable callus composed of free round vacuolated and undifferentiated cellse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata subsp. unguiculata
Vigna unguiculata subsp. unguiculata · TaxonWibowo Djatmiko ( Wie146 ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“High concentration (22.1μM) of 2,4-D caused watery friable callus composed of free round vacuolated and undifferentiated cells.”

✓ located verbatim in the source · abstract, characters 341–468 · show in text ↑
t0

“Vigna sinensis”

✓ located verbatim in the source · title, characters 65–79 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Kinetin reduced free cell formation.

Treatment
kinetine0
Outcome
free cell formatione0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata subsp. unguiculata
Vigna unguiculata subsp. unguiculata · TaxonWibowo Djatmiko ( Wie146 ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“an addition of kinetin decreased free cell formation and gave compact tissue.”

✓ located verbatim in the source · abstract, characters 488–565 · show in text ↑
t0

“Vigna sinensis”

✓ located verbatim in the source · title, characters 65–79 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Kinetin produced compact tissue.

Treatment
kinetine0
Outcome
compact tissuee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata subsp. unguiculata
Vigna unguiculata subsp. unguiculata · TaxonWibowo Djatmiko ( Wie146 ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“an addition of kinetin decreased free cell formation and gave compact tissue.”

✓ located verbatim in the source · abstract, characters 488–565 · show in text ↑
t0

“Vigna sinensis”

✓ located verbatim in the source · title, characters 65–79 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

High kinetin levels induced differentiation to tracheal elements.

Treatment
High level (10 and 40μM) of kinetine0
Outcome
cell differentiation to tracheal elemente0
Direction
inducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata subsp. unguiculata
Vigna unguiculata subsp. unguiculata · TaxonWibowo Djatmiko ( Wie146 ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“High level (10 and 40μM) of kinetin induced cell differentiation to tracheal element”

✓ located verbatim in the source · abstract, characters 566–650 · show in text ↑
t0

“Vigna sinensis”

✓ located verbatim in the source · title, characters 65–79 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

At low 2,4-D, 40μM kinetin inhibited tracheal element formation.

Treatment
40μM kinetine0
Outcome
formation of tracheal elemente0
Direction
inhibitede0
Qualifier
low level (0 or 0.0221μM) of 2,4-De0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata subsp. unguiculata
Vigna unguiculata subsp. unguiculata · TaxonWibowo Djatmiko ( Wie146 ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“40μM kinetin rather inhibited formation of tracheal element in the case of low level (0 or 0.0221μM) of 2,4-D.”

✓ located verbatim in the source · abstract, characters 656–766 · show in text ↑
t0

“Vigna sinensis”

✓ located verbatim in the source · title, characters 65–79 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Nicotinic acid did not affect tissue texture, cell characteristics or differentiation.

Treatment
Nicotinic acide0
Outcome
tissue texture, cell characteristics and differentiatione0
Direction
no effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata subsp. unguiculata
Vigna unguiculata subsp. unguiculata · TaxonWibowo Djatmiko ( Wie146 ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Nicotinic acid had no effect on tissue texture, cell characteristics and differentiation”

✓ located verbatim in the source · abstract, characters 868–956 · show in text ↑
t0

“Vigna sinensis”

✓ located verbatim in the source · title, characters 65–79 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Nicotinic acid strongly stimulated cell proliferation.

Treatment
Nicotinic acide0
Outcome
cell proliferatione0
Direction
stimulated stronglye0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata subsp. unguiculata
Vigna unguiculata subsp. unguiculata · TaxonWibowo Djatmiko ( Wie146 ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Nicotinic acid had no effect on tissue texture, cell characteristics and differentiation, although stimulated strongly cell proliferation.”

✓ located verbatim in the source · abstract, characters 868–1006 · show in text ↑
t0

“Vigna sinensis”

✓ located verbatim in the source · title, characters 65–79 · show in text ↑

2,4-D · growth · Vigna unguiculata (cowpea)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

3 in the opposite direction

  1. positive
  2. positive
  3. positive
See all 4 papers →

1-naphthaleneacetic acid · growth · Vigna unguiculata (cowpea)Positive and negative results

14 papers: 13 positive, 1 negative or no effect

13 in the opposite direction

  1. positive
  2. positive
  3. positive

10 papers more in this group

See all 14 papers →

nicotinic acid · growth · Vigna unguiculata (cowpea)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

class · growth · Vigna unguiculata (cowpea)Positive and negative results

7 papers: 5 positive, 2 negative or no effect

4 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. no effect
  3. positive

3 papers more in this group

See all 7 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.