Abstract
AtR8 lncRNA was previously identified in the flowering plant Arabidopsis thaliana as an abundant Pol III-transcribed long non-coding RNA (lncRNA) of approximately 260 nt. AtR8 lncRNA accumulation is responsive to hypoxic stress and salicylic acid (SA) treatment in roots, but its function has not yet been identified. In this study, microarray analysis of an atr8 mutant and wild-type Arabidopsis indicated a strong association of AtR8 lncRNA with the defense response. AtR8 accumulation exhibited an inverse correlation with an accumulation of two WRKY genes (WRKY53/WRKY70) when plants were exposed to exogenous low SA concentrations (20 µM), infected with Pseudomonas syringae, or in the early stage of development. The highest AtR8 accumulation was observed 5 days after germination, at which time no WRKY53 or WRKY70 mRNA was detectable. The presence of low levels of SA resulted in a significant reduction of root length in atr8 seedlings, whereas wrky53 and wrky70 mutants exhibited the opposite phenotype. Taken together, AtR8 lncRNA participates in Pathogenesis-Related Proteins 1 (PR-1)-independent defense and root elongation, which are related to the SA response. The mutual regulation of AtR8 lncRNA and WRKY53/WRKY70 is mediated by Nonexpressor of Pathogenesis-Related Gene 1 (NPR1).
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Claims 8
Claim · model-written summary
Microarray analysis associated AtR8 with the defense response.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- atr8 mutante0
- Gene
- AtR8 lncRNAe0
- Outcome
- defense responsee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“microarray analysis of an atr8 mutant and wild-type Arabidopsis indicated a strong association of AtR8 lncRNA with the defense response”
Claim · model-written summary
AtR8 accumulation inversely correlated with WRKY53/WRKY70 accumulation under low SA, infection, or early development.
- Taxon
- plantsViridiplantaee0
- Treatment
- exogenous low SA concentrations (20 µM)e0
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee0pathogen - Gene
- WRKY53/WRKY70e0
- Outcome
- AtR8 accumulatione0
- Direction
- inverse correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtR8 accumulation exhibited an inverse correlation with an accumulation of two WRKY genes (WRKY53/WRKY70) when plants were exposed to exogenous low SA concentrations (20 µM), infected with Pseudomonas syringae, or in the early stage of development.”
Claim · model-written summary
AtR8 accumulation peaked five days after germination.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest AtR8 accumulation was observed 5 days after germination”
t0“Arabidopsis”
Claim · model-written summary
WRKY53 and WRKY70 mRNA were undetectable when AtR8 accumulation peaked.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- WRKY53 or WRKY70e0
- Outcome
- WRKY53 or WRKY70 mRNA was detectablee0
- Direction
- noe0
- Qualifier
- 5 days after germinatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at which time no WRKY53 or WRKY70 mRNA was detectable”
e1“The highest AtR8 accumulation was observed 5 days after germination”
t0“Arabidopsis”
Claim · model-written summary
Low salicylic acid significantly shortened roots of atr8 seedlings.
- Taxon
- ArabidopsisArabidopsist0
- Material
- atr8 seedlingse0
- Treatment
- low levels of SAe0
- Outcome
- root lengthe0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of low levels of SA resulted in a significant reduction of root length in atr8 seedlings”
t0“Arabidopsis”
Claim · model-written summary
wrky53 and wrky70 mutants showed the opposite root-length phenotype under low SA.
- Taxon
- ArabidopsisArabidopsist0
- Material
- wrky53 and wrky70 mutantse0
- Treatment
- low levels of SAe1
- Outcome
- opposite phenotypee0
- Comparator
- atr8 seedlingse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas wrky53 and wrky70 mutants exhibited the opposite phenotype”
e1“The presence of low levels of SA resulted in a significant reduction of root length in atr8 seedlings”
t0“Arabidopsis”
Claim · model-written summary
AtR8 participates in PR-1-independent defense and root elongation linked to SA response.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtR8 lncRNAe0
- Compound
- SAe0
- Outcome
- defense and root elongatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtR8 lncRNA participates in Pathogenesis-Related Proteins 1 (PR-1)-independent defense and root elongation, which are related to the SA response.”
t0“Arabidopsis”
Claim · model-written summary
NPR1 mediates mutual regulation of AtR8 and WRKY53/WRKY70.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- Nonexpressor of Pathogenesis-Related Gene 1 (NPR1)e0
- Outcome
- mutual regulation of AtR8 lncRNA and WRKY53/WRKY70e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mutual regulation of AtR8 lncRNA and WRKY53/WRKY70 is mediated by Nonexpressor of Pathogenesis-Related Gene 1 (NPR1).”
t0“Arabidopsis”
The same questions in other papers
salicylic acid · growth · ArabidopsisPositive and negative results
4 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.