Abstract
Summary Low oxygen availability often is associated with soil waterlogging or submergence, but may occur also as hypoxic niches in otherwise aerobic tissues. Experimental evidence assigns a role in Botrytis cinerea resistance to a group of oxygen‐unstable Ethylene Response Factors (ERF‐VII). Given that infection by B. cinerea often occurs in aerobic organs such as leaves, where ERF‐VII stability should be compromised, we explored the possibility of local leaf hypoxia at the site of infection. We analyzed the expression of hypoxia‐responsive genes in infected leaves. Confocal microscopy was utilized to verify the localization of the ERF‐VII protein RAP2.12. Oxygen concentration was measured to evaluate the availability of oxygen (O 2 ). We discovered that infection by B. cinerea induces increased respiration, leading to a drastic drop in the O 2 concentration in an otherwise fully aerobic leaf. The establishment of a local hypoxic area results in stabilization and nuclear relocalization of RAP2.12. The possible roles of defence elicitors, ABA and ethylene were evaluated. Local hypoxia at the site of B. cinerea infection allows the stabilization of ERF‐VII proteins. Hypoxia at the site of pathogen infection generates a nearly O 2 ‐free environment that may affect the stability of other N‐degron‐regulated proteins as well as the metabolism of elicitors.
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Claims 5
Claim · model-written summary
Botrytis infection increased respiration in Arabidopsis leaves.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- infection by B. cinereae0
- Interactor
- B. cinerea
Botrytis cinereae0pathogen - Outcome
- respiratione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infection by B. cinerea induces increased respiration”
t0“Arabidopsis”
Claim · model-written summary
Botrytis infection caused a drastic drop in leaf oxygen concentration.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- leafe0
- Treatment
- infection by B. cinereae0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- O 2 concentratione0
- Direction
- drastic drope0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infection by B. cinerea induces increased respiration, leading to a drastic drop in the O 2 concentration in an otherwise fully aerobic leaf.”
t0“Arabidopsis”
Claim · model-written summary
Local hypoxia stabilized RAP2.12.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RAP2.12e0
- Outcome
- stabilizatione0
- Qualifier
- local hypoxic areae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The establishment of a local hypoxic area results in stabilization and nuclear relocalization of RAP2.12.”
t0“Arabidopsis”
Claim · model-written summary
Local hypoxia caused nuclear relocalization of RAP2.12.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RAP2.12e0
- Outcome
- nuclear relocalizatione0
- Qualifier
- local hypoxic areae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The establishment of a local hypoxic area results in stabilization and nuclear relocalization of RAP2.12.”
t0“Arabidopsis”
Claim · model-written summary
The infection site became a nearly oxygen-free environment.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- nearly O 2 ‐free environmente0
Not reportedOrgan / stage
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypoxia at the site of pathogen infection generates a nearly O 2 ‐free environment”
t0“Arabidopsis”
The same questions in other papers
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infection · molecular: metabolites · ArabidopsisPositive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
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6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.