Abstract
Abstract This study aimed to reveal the mechanistic basis of the melatonin‐mediated amelioration of salinity stress in plants. Electrophysiological experiments revealed that melatonin decreased salt‐induced K + efflux (a critical determinant of plant salt tolerance) in a dose‐ and time‐dependent manner and reduced sensitivity of the plasma membrane K + ‐permeable channels to hydroxyl radicals. These beneficial effects of melatonin were abolished by NADPH oxidase blocker DPI. Transcriptome analyses revealed that melatonin induced 585 (448 up‐ and 137 down‐regulated) and 59 (54 up‐ and 5 down‐regulated) differentially expressed genes (DEGs) in the root tip and mature zone, respectively. The most noticeable changes in the root tip were melatonin‐induced increase in the expression of several DEGs encoding respiratory burst NADPH oxidases ( OsRBOHA and OsRBOHF ), calcineurin B‐like/calcineurin B‐like‐interacting protein kinase ( OsCBL / OsCIPK ), and calcium‐dependent protein kinase ( OsCDPK ) under salt stress. Melatonin also enhanced the expression of potassium transporter genes ( OsAKT1 , OsHAK1 , and OsHAK5 ). Taken together, these results indicate that melatonin improves salt tolerance in rice by enabling K + retention in roots, and that the latter process is conferred by melatonin scavenging of hydroxyl radicals and a concurrent OsRBOHF ‐dependent ROS signalling required to activate stress‐responsive genes and increase the expression of K + uptake transporters in the root tip.
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Claims 8
Claim · model-written summary
Melatonin decreased salt-induced potassium efflux with dose and time.
- Taxon
- rice
Oryza sativat0 - Treatment
- melatonine0
- Outcome
- salt‐induced K + effluxe0
- Direction
- decreasede0
- Qualifier
- dose‐ and time‐dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“melatonin decreased salt‐induced K + efflux (a critical determinant of plant salt tolerance) in a dose‐ and time‐dependent manner”
t0“rice”
Claim · model-written summary
Melatonin reduced K-channel sensitivity to hydroxyl radicals.
- Taxon
- riceOryza sativat0
- Treatment
- melatonine0
- Outcome
- sensitivity of the plasma membrane K + ‐permeable channels to hydroxyl radicalse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“melatonin decreased salt‐induced K + efflux (a critical determinant of plant salt tolerance) in a dose‐ and time‐dependent manner and reduced sensitivity of the plasma membrane K + ‐permeable channels to hydroxyl radicals”
t0“rice”
Claim · model-written summary
NADPH oxidase blocker DPI abolished the beneficial effects of melatonin.
- Taxon
- riceOryza sativat0
- Treatment
- NADPH oxidase blocker DPIe0
- Outcome
- beneficial effects of melatonine0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These beneficial effects of melatonin were abolished by NADPH oxidase blocker DPI”
t0“rice”
Claim · model-written summary
Melatonin induced 585 differentially expressed genes in the root tip, with 448 upregulated and 137 downregulated.
- Taxon
- riceOryza sativat0
- Organ / stage
- root tipe0
- Treatment
- melatonine0
- Outcome
- differentially expressed genes (DEGs) in the root tipe0
- Direction
- inducede0
- Value
- 585e0
- Qualifier
- 448 up‐ and 137 down‐regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“melatonin induced 585 (448 up‐ and 137 down‐regulated) and 59 (54 up‐ and 5 down‐regulated) differentially expressed genes (DEGs) in the root tip and mature zone, respectively”
t0“rice”
Claim · model-written summary
Melatonin induced 59 differentially expressed genes in the mature zone, with 54 upregulated and 5 downregulated.
- Taxon
- riceOryza sativat0
- Organ / stage
- mature zonee0
- Treatment
- melatonine0
- Outcome
- differentially expressed genes (DEGs)e0
- Direction
- inducede0
- Value
- 59e0
- Qualifier
- 54 up‐ and 5 down‐regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“melatonin induced 585 (448 up‐ and 137 down‐regulated) and 59 (54 up‐ and 5 down‐regulated) differentially expressed genes (DEGs) in the root tip and mature zone, respectively”
t0“rice”
Claim · model-written summary
Under salt stress melatonin increased expression of OsRBOHA and OsRBOHF in root tips.
- Taxon
- riceOryza sativat0
- Treatment
- melatonine0
- Gene
- OsRBOHA and OsRBOHFe0
- Outcome
- expressione0
- Direction
- increasee0
- Qualifier
- under salt stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“melatonin‐induced increase in the expression of several DEGs encoding respiratory burst NADPH oxidases ( OsRBOHA and OsRBOHF ), calcineurin B‐like/calcineurin B‐like‐interacting protein kinase ( OsCBL / OsCIPK ), and calcium‐dependent protein kinase ( OsCDPK ) under salt stress”
t0“rice”
Claim · model-written summary
Under salt stress melatonin increased expression of OsCBL/OsCIPK and OsCDPK in root tips.
- Taxon
- riceOryza sativat0
- Treatment
- melatonine0
- Gene
- OsCBL / OsCIPKe0
- Outcome
- expressione0
- Direction
- increasee0
- Qualifier
- under salt stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“melatonin‐induced increase in the expression of several DEGs encoding respiratory burst NADPH oxidases ( OsRBOHA and OsRBOHF ), calcineurin B‐like/calcineurin B‐like‐interacting protein kinase ( OsCBL / OsCIPK ), and calcium‐dependent protein kinase ( OsCDPK ) under salt stress”
t0“rice”
Claim · model-written summary
Melatonin enhanced expression of OsAKT1, OsHAK1 and OsHAK5.
- Taxon
- riceOryza sativat0
- Treatment
- Melatonine0
- Gene
- OsAKT1 , OsHAK1 , and OsHAK5e0
- Outcome
- expression of potassium transporter genese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Melatonin also enhanced the expression of potassium transporter genes ( OsAKT1 , OsHAK1 , and OsHAK5 )”
t0“rice”
The same questions in other papers
melatonin · molecular: metabolites · Oryza sativa (rice)Positive and negative results
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- positive
- no effect
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See all 16 papers →melatonin · molecular: expression · Oryza sativa (rice)Results agree
19 papers in the same direction as this paper
- positive
- positive
- positive
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See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.