Abstract
BACKGROUND: "Bairihua", a variety of the Catalpa bungei, has a large amount of flowers and a long flowering period which make it an excellent material for flowering researches in trees. SPL is one of the hub genes that regulate both flowering transition and development. RESULTS: SPL homologues CbuSPL9 was cloned using degenerate primers with RACE. Expression studies during flowering transition in "Bairihua" and ectopic expression in Arabidopsis showed that CbuSPL9 was functional similarly with its Arabidopsis homologues. In the next step, we used Y2H to identify the proteins that could interact with CbuSPL9. HMGA, an architectural transcriptional factor, was identified and cloned for further research. BiFC and BLI showed that CbuSPL9 could form a heterodimer with CbuHMGA in the nucleus. The expression analysis showed that CbuHMGA had a similar expression trend to that of CbuSPL9 during flowering in "Bairihua". Intriguingly, ectopic expression of CbuHMGA in Arabidopsis would lead to aberrant flowers, but did not effect flowering time. CONCLUSIONS: Our results implied a novel pathway that CbuSPL9 regulated flowering development, but not flowering transition, with the participation of CbuHMGA. Further investments need to be done to verify the details of this pathway.
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Claims 8
Claim · model-written summary
CbuSPL9 was functionally similar to its Arabidopsis homologues in expression studies in Bairihua and in ectopic expression in Arabidopsis.
- Taxon
- Catalpa bungei
Catalpa bungeit0 - Material
- Bairihuae1
- Gene
- CbuSPL9e1
- Outcome
- functional similarly with its Arabidopsis homologuese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flowering in Catalpa bungei”
e1“Expression studies during flowering transition in "Bairihua" and ectopic expression in Arabidopsis showed that CbuSPL9 was functional similarly with its Arabidopsis homologues”
Claim · model-written summary
CbuSPL9 formed a heterodimer with CbuHMGA in the nucleus (BiFC and BLI).
- Taxon
- Catalpa bungeiCatalpa bungeit0
- Gene
- CbuSPL9e2
- Outcome
- heterodimer with CbuHMGAe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flowering in Catalpa bungei”
e2“BiFC and BLI showed that CbuSPL9 could form a heterodimer with CbuHMGA in the nucleus”
Claim · model-written summary
CbuHMGA had an expression trend during flowering in Bairihua similar to that of CbuSPL9.
- Taxon
- Catalpa bungeiCatalpa bungeit0
- Material
- Bairihuae3
- Gene
- CbuHMGAe3
- Outcome
- expression trende3
- Direction
- similare3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flowering in Catalpa bungei”
e3“The expression analysis showed that CbuHMGA had a similar expression trend to that of CbuSPL9 during flowering in "Bairihua"”
Claim · model-written summary
Ectopic expression of CbuHMGA in Arabidopsis led to aberrant flowers.
- Taxon
- Arabidopsis
Arabidopsise4 - Treatment
- ectopic expression of CbuHMGAe4
- Gene
- CbuHMGAe4
- Outcome
- aberrant flowerse4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“ectopic expression of CbuHMGA in Arabidopsis would lead to aberrant flowers, but did not effect flowering time”
Claim · model-written summary
Ectopic expression of CbuHMGA in Arabidopsis did not affect flowering time.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- ectopic expression of CbuHMGAe4
- Gene
- CbuHMGAe4
- Outcome
- flowering timee4
- Direction
- did not effecte4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“ectopic expression of CbuHMGA in Arabidopsis would lead to aberrant flowers, but did not effect flowering time”
Claim · model-written summary
The authors infer that CbuSPL9 regulates flowering development but not flowering transition, with CbuHMGA participating.
- Taxon
- Catalpa bungeiCatalpa bungeit0
- Gene
- CbuSPL9e5
- Outcome
- flowering developmente5
- Qualifier
- with the participation of CbuHMGAe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flowering in Catalpa bungei”
e5“Our results implied a novel pathway that CbuSPL9 regulated flowering development, but not flowering transition, with the participation of CbuHMGA”
Claim · model-written summary
An SPL homologue, CbuSPL9, was cloned using degenerate primers with RACE.
- Taxon
- Catalpa bungeiCatalpa bungeit0
- Gene
- CbuSPL9e6
- Outcome
- clonede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flowering in Catalpa bungei”
e6“SPL homologues CbuSPL9 was cloned using degenerate primers with RACE”
Claim · model-written summary
HMGA, an architectural transcriptional factor, was identified by Y2H and cloned.
- Taxon
- Catalpa bungeiCatalpa bungeit0
- Gene
- HMGAe7
- Outcome
- identified and clonede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flowering in Catalpa bungei”
e7“HMGA, an architectural transcriptional factor, was identified and cloned for further research”
The same questions in other papers
overexpression · phenology · ArabidopsisPositive and negative results
4 papers in the same direction as this paper · 27 in the opposite direction
- positive
- positive
- negative
28 papers more in this group
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