Abstract
Abstract Salt stress activates defence responses in plants, including changes in leaf surface structure. Here, we showed that the transcriptional activation of cutin deposition and antioxidant defence by the R2R3‐type MYB transcription factor AtMYB49 contributed to salt tolerance in Arabidopsis thaliana . Characterization of loss‐of‐function myb49 mutants, and chimeric AtMYB49‐SRDX‐ overexpressing SRDX49 transcriptional repressor and AtMYB49 ‐overexpressing ( OX49 ) overexpressor plants demonstrated a positive role of AtMYB49 in salt tolerance. Transcriptome analysis revealed that many genes belonging to the category “cutin, suberin and wax biosyntheses” were markedly up‐regulated and down‐regulated in OX49 and SRDX49 plants, respectively, under normal and/or salt stress conditions. Some of these differentially expressed genes, including MYB41 , ASFT , FACT and CYP86B1 , were also shown to be the direct targets of AtMYB49 and activated by AtMYB49. Biochemical analysis indicated that AtMYB49 modulated cutin deposition in the leaves. Importantly, cuticular transpiration, chlorophyll leaching and toluidine blue‐staining assays revealed a link between increased AtMYB49‐mediated cutin deposition in leaves and enhanced salt tolerance. Additionally, increased AtMYB49 expression elevated Ca 2+ level in leaves and improved antioxidant capacity by up‐regulating genes encoding peroxidases and late embryogenesis abundant proteins. These results suggest that genetic manipulation of AtMYB49 may provide a novel way to improve salt tolerance in plants.
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Claims 10
Claim · model-written summary
AtMYB49 contributed to salt tolerance in Arabidopsis thaliana.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- AtMYB49e0
- Outcome
- salt tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMYB49 contributed to salt tolerance in Arabidopsis thaliana”
Claim · model-written summary
Overexpression line OX49 up-regulated many cutin, suberin and wax biosynthesis genes under normal and salt stress conditions.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- OX49e1
- Treatment
- salt stress conditionse1
- Outcome
- many genes belonging to the categorye1
- Direction
- up‐regulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMYB49 contributed to salt tolerance in Arabidopsis thaliana”
e1“many genes belonging to the category “cutin, suberin and wax biosyntheses” were markedly up‐regulated and down‐regulated in OX49 and SRDX49 plants, respectively, under normal and/or salt stress conditions”
Claim · model-written summary
Repressor line SRDX49 down-regulated many cutin, suberin and wax biosynthesis genes under normal and salt stress conditions.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- SRDX49e1
- Treatment
- salt stress conditionse1
- Outcome
- many genes belonging to the categorye1
- Direction
- down‐regulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMYB49 contributed to salt tolerance in Arabidopsis thaliana”
e1“many genes belonging to the category “cutin, suberin and wax biosyntheses” were markedly up‐regulated and down‐regulated in OX49 and SRDX49 plants, respectively, under normal and/or salt stress conditions”
Claim · model-written summary
MYB41, ASFT and FACT are direct targets of AtMYB49 and are activated by it in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- MYB41 , ASFT , FACTe2
- Outcome
- direct targets of AtMYB49e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMYB49 contributed to salt tolerance in Arabidopsis thaliana”
e2“including MYB41 , ASFT , FACT and CYP86B1, were also shown to be the direct targets of AtMYB49 and activated by AtMYB49”
Claim · model-written summary
CYP86B1 is a direct target of AtMYB49 and is activated by it in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- CYP86B1e2
- Outcome
- direct targets of AtMYB49e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMYB49 contributed to salt tolerance in Arabidopsis thaliana”
e2“including MYB41 , ASFT , FACT and CYP86B1, were also shown to be the direct targets of AtMYB49 and activated by AtMYB49”
Claim · model-written summary
AtMYB49 modulated cutin deposition in Arabidopsis leaves.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- leavese3
- Gene
- AtMYB49e3
- Outcome
- cutin depositione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMYB49 contributed to salt tolerance in Arabidopsis thaliana”
e3“Biochemical analysis indicated that AtMYB49 modulated cutin deposition in the leaves”
Claim · model-written summary
Increased AtMYB49-mediated cutin deposition in leaves was linked to enhanced salt tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- leavese4
- Gene
- AtMYB49e4
- Outcome
- salt tolerancee4
- Direction
- enhancede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMYB49 contributed to salt tolerance in Arabidopsis thaliana”
e4“a link between increased AtMYB49‐mediated cutin deposition in leaves and enhanced salt tolerance”
Claim · model-written summary
Increased AtMYB49 expression elevated the Ca 2+ level in Arabidopsis leaves.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- leavese5
- Treatment
- increased AtMYB49 expressione5
- Outcome
- Ca 2+ levele5
- Direction
- elevatede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMYB49 contributed to salt tolerance in Arabidopsis thaliana”
e5“increased AtMYB49 expression elevated Ca 2+ level in leaves”
Claim · model-written summary
AtMYB49 improved antioxidant capacity in Arabidopsis by up-regulating peroxidase and late embryogenesis abundant protein genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- peroxidases and late embryogenesis abundant proteinse6
- Outcome
- antioxidant capacitye6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMYB49 contributed to salt tolerance in Arabidopsis thaliana”
e6“improved antioxidant capacity by up‐regulating genes encoding peroxidases and late embryogenesis abundant proteins”
Claim · model-written summary
Genetic manipulation of AtMYB49 may improve salt tolerance in plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- improve salt tolerance in plantse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMYB49 contributed to salt tolerance in Arabidopsis thaliana”
e7“genetic manipulation of AtMYB49 may provide a novel way to improve salt tolerance in plants”
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