Abstract
Legumes establish symbiotic relationships with soil bacteria (rhizobia), housed in nodules on roots. The plant supplies carbon substrates and other nutrients to the bacteria in exchange for fixed nitrogen. The exchange occurs across a plant-derived symbiosome membrane (SM), which encloses rhizobia to form a symbiosome. Iron supplied by the plant is crucial for rhizobial enzyme nitrogenase that catalyses nitrogen fixation, but the SM iron transporter has not been identified. We use yeast complementation, real-time PCR and proteomics to study putative soybean (Glycine max) iron transporters GmVTL1a and GmVTL1b and have characterized the role of GmVTL1a using complementation in plant mutants, hairy root transformation and microscopy. GmVTL1a and GmVTL1b are members of the vacuolar iron transporter family and homologous to Lotus japonicus SEN1 (LjSEN1), which is essential for nitrogen fixation. GmVTL1a expression is enhanced in nodule infected cells and both proteins are localized to the SM. GmVTL1a transports iron in yeast and restores nitrogen fixation when expressed in the Ljsen1 mutant. Three GmVTL1a amino acid substitutions that block nitrogen fixation in Ljsen1 plants reduce iron transport in yeast. We conclude GmVTL1a is responsible for transport of iron across the SM to bacteroids and plays a crucial role in the nitrogen-fixing symbiosis.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
GmVTL1a and GmVTL1b are members of the vacuolar iron transporter family in soybean.
- Taxon
- soybean
Glycine maxt0 - Gene
- GmVTL1a and GmVTL1be1
- Outcome
- members of the vacuolar iron transporter familye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“GmVTL1a is an iron transporter on the symbiosome membrane of soybean”
e1“GmVTL1a and GmVTL1b are members of the vacuolar iron transporter family and homologous to Lotus japonicus SEN1 (LjSEN1), which is essential for nitrogen fixation”
Claim · model-written summary
GmVTL1a expression is enhanced in soybean nodule infected cells.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- nodule infected cellse2
- Gene
- GmVTL1ae2
- Outcome
- GmVTL1a expressione2
- Direction
- enhancede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“GmVTL1a is an iron transporter on the symbiosome membrane of soybean”
e2“GmVTL1a expression is enhanced in nodule infected cells and both proteins are localized to the SM”
Claim · model-written summary
GmVTL1a is localized to the symbiosome membrane in soybean.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmVTL1ae2
- Outcome
- localized to the SMe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“GmVTL1a is an iron transporter on the symbiosome membrane of soybean”
e2“GmVTL1a expression is enhanced in nodule infected cells and both proteins are localized to the SM”
Claim · model-written summary
GmVTL1a transports iron in yeast.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmVTL1ae3
- Outcome
- transports irone3
- Setting
- yeaste3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“GmVTL1a is an iron transporter on the symbiosome membrane of soybean”
e3“GmVTL1a transports iron in yeast and restores nitrogen fixation when expressed in the Ljsen1 mutant”
Claim · model-written summary
Expressing GmVTL1a in the Ljsen1 mutant restores nitrogen fixation.
- Taxon
- soybeanGlycine maxt0
- Material
- Ljsen1 mutante3
- Treatment
- expressed in the Ljsen1 mutante3
- Gene
- GmVTL1ae3
- Outcome
- nitrogen fixatione3
- Direction
- restorese3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“GmVTL1a is an iron transporter on the symbiosome membrane of soybean”
e3“GmVTL1a transports iron in yeast and restores nitrogen fixation when expressed in the Ljsen1 mutant”
Claim · model-written summary
Three GmVTL1a amino acid substitutions reduce iron transport in yeast.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Three GmVTL1a amino acid substitutionse4
- Gene
- GmVTL1ae4
- Outcome
- iron transport in yeaste4
- Direction
- reducee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“GmVTL1a is an iron transporter on the symbiosome membrane of soybean”
e4“Three GmVTL1a amino acid substitutions that block nitrogen fixation in Ljsen1 plants reduce iron transport in yeast”
Claim · model-written summary
Three GmVTL1a amino acid substitutions block nitrogen fixation in Ljsen1 plants.
- Taxon
- soybeanGlycine maxt0
- Material
- Ljsen1 plantse4
- Treatment
- Three GmVTL1a amino acid substitutionse4
- Gene
- GmVTL1ae4
- Outcome
- nitrogen fixatione4
- Direction
- blocke4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“GmVTL1a is an iron transporter on the symbiosome membrane of soybean”
e4“Three GmVTL1a amino acid substitutions that block nitrogen fixation in Ljsen1 plants reduce iron transport in yeast”
Claim · model-written summary
GmVTL1a is responsible for iron transport across the symbiosome membrane to bacteroids and plays a crucial role in nitrogen-fixing symbiosis in soybean.
- Taxon
- soybeanGlycine maxt0
- Interactor
- bacteroidse5symbiont
- Gene
- GmVTL1ae5
- Outcome
- nitrogen-fixing symbiosise5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“GmVTL1a is an iron transporter on the symbiosome membrane of soybean”
e5“We conclude GmVTL1a is responsible for transport of iron across the SM to bacteroids and plays a crucial role in the nitrogen-fixing symbiosis”
The same questions in other papers
amino acid · nutrient: content · Glycine max (soybean)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.