Abstract
Abstract Background and objective The main objective of the present study was production of xylanase from different agricultural wastes using Chaetomium globosum on pomegranate waste, isolation of fungi associated with some legumes seeds, and studied the effect of safe product which obtained from xylanse under laboratory and greenhouse conditions on bean seeds. Results and discussion Different agricultural wastes were tested for xylanase production by Ch. globosum ; from them, pomegranate peel was most effective at concentration 40 g/l using 2 disks 6 mm in diameter after 7-day incubation period produces 1398.34 U/ml. Addition of calcium chloride increases xylanase activity to 1469.40 U/ml. The crude xylanase activity was active after 20 min of starting of reaction at 6.6 pH, and 40 °C of the reaction mixture produces 1587.27 U/ml. Xylanse is still active at 40 °C for 30 min. Seeds of some legumes were examined for seed borne mycoflora by the agar plate method. The most common fungi were Fusarium solani , Rhizoctonia solani , and Macrophomina phaseolina . Testing of crude xylanase as biocide was more effective in reduction of linear growth of some pathogenic fungi also applied the biocide as seed treatment of bean. Seed coating with biocide gave significant protection to emerge bean seeds against invasion of pathogenic fungi at the pre-emergence stage. Seed coating recorded more than 50% protection compared with the untreated control. Conclusion Production of xylanase from pomegranate peel wastes using Chaetomium globosum under optimum conditions. Testing crude xylanase after optimization as an effective biocide.
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Claims 8
Claim · model-written summary
Pomegranate peel gave the most effective xylanase production by Ch. globosum at the specified conditions, yielding 1398.34 U/ml.
Not reportedSettingDirection
Verbatim
e0“pomegranate peel was most effective at concentration 40 g/l using 2 disks 6 mm in diameter after 7-day incubation period produces 1398.34 U/ml”
Claim · model-written summary
Calcium chloride increased xylanase activity to 1469.40 U/ml.
Not reportedTaxonComparator
Verbatim
e0“Addition of calcium chloride increases xylanase activity to 1469.40 U/ml”
Claim · model-written summary
Crude xylanase activity reached 1587.27 U/ml at 6.6 pH and 40 °C.
Not reportedTaxonDirection
Verbatim
e0“The crude xylanase activity was active after 20 min of starting of reaction at 6.6 pH, and 40 °C of the reaction mixture produces 1587.27 U/ml”
Claim · model-written summary
Crude xylanase remained active at 40 °C for 30 min.
- Outcome
- Xylanse is still activee0
Not reportedTaxonDirection
Verbatim
e0“Xylanse is still active at 40 °C for 30 min”
Claim · model-written summary
Fusarium solani, Rhizoctonia solani and Macrophomina phaseolina were the most common seed-borne fungi in legumes.
- Outcome
- most common fungie0
Not reportedTaxonDirection
Verbatim
e0“The most common fungi were Fusarium solani , Rhizoctonia solani , and Macrophomina phaseolina”
Claim · model-written summary
Crude xylanase reduced linear growth of pathogenic fungi.
Not reportedTaxonComparator
Verbatim
e0“Testing of crude xylanase as biocide was more effective in reduction of linear growth of some pathogenic fungi”
Claim · model-written summary
Bean seed coating with biocide significantly protected emerging seeds from pathogenic fungi at pre-emergence.
- Taxon
- beane0
- Organ / stage
- pre-emergence stagee0
- Treatment
- Seed coating with biocidee0
- Outcome
- invasion of pathogenic fungie0
- Direction
- protectione0
Not reportedComparator
Verbatim
e0“Seed coating with biocide gave significant protection to emerge bean seeds against invasion of pathogenic fungi at the pre-emergence stage”
Claim · model-written summary
Bean seed coating provided more than 50% protection relative to untreated control.
Verbatim
e0“Seed coating recorded more than 50% protection compared with the untreated control”
t0“bean”
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