Abstract
Plant phenotypes caused by mineral deficiencies differ depending on growth conditions. We recently reported that the growth of Arabidopsis thaliana was severely inhibited on MGRL-based zinc (Zn)-deficient medium but not on Murashige-Skoog-based Zn-deficient medium. Here, we explored the underlying reason for the phenotypic differences in Arabidopsis grown on the different media. The root growth and chlorophyll contents reduced by Zn deficiency were rescued by the addition of extra manganese (Mn) during short-term growth (10 or 14 d). However, this treatment did not affect the growth recovery after long-term growth (38 d). To investigate the reason for plant recovery from Zn deficiency, we performed the RNA-seq analysis of the roots grown on the Zn-basal medium and the Zn-depleted medium with/without additional Mn. Principal component analysis of the RNA-seq data showed that the gene expression patterns of plants on the Zn-basal medium were similar to those on the Zn-depleted medium with Mn, whereas those on the Zn-depleted medium without Mn were different from the others. The expression of several transcription factors and reactive oxygen species (ROS)-related genes was upregulated in only plants on the Zn-depleted medium without Mn. Consistent with the gene expression data, ROS accumulation in the roots grown on this medium was higher than those grown in other conditions. These results suggest that plants accumulate ROS and reduce their biomass under undesirable growth conditions, such as Zn depletion. Taken together, this study shows that the addition of extra Mn to the Zn-depleted medium induces transcriptional changes in ROS-related genes, thereby alleviating short-term growth inhibition due to Zn deficiency.
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Claims 7
Claim · model-written summary
Arabidopsis growth was severely inhibited on MGRL-based zinc-deficient medium but not on Murashige-Skoog-based medium.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- MGRL-based zinc (Zn)-deficient mediume0
- Outcome
- growthe0
- Direction
- severely inhibitede0
- Comparator
- Murashige-Skoog-based Zn-deficient mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the growth of Arabidopsis thaliana was severely inhibited on MGRL-based zinc (Zn)-deficient medium but not on Murashige-Skoog-based Zn-deficient medium”
Claim · model-written summary
Extra manganese rescued root growth reduced by zinc deficiency during 10 or 14 days of growth.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- addition of extra manganese (Mn)e0
- Outcome
- root growthe0
- Direction
- rescuede0
- Qualifier
- short-term growth (10 or 14 d)e0
- Comparator
- Zn deficiencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The root growth and chlorophyll contents reduced by Zn deficiency were rescued by the addition of extra manganese (Mn) during short-term growth (10 or 14 d).”
t0“Arabidopsis Seedlings”
Claim · model-written summary
Extra manganese rescued chlorophyll contents reduced by zinc deficiency during 10 or 14 days.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- addition of extra manganese (Mn)e0
- Outcome
- chlorophyll contentse0
- Direction
- rescuede0
- Comparator
- Zn deficiencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The root growth and chlorophyll contents reduced by Zn deficiency were rescued by the addition of extra manganese (Mn) during short-term growth (10 or 14 d).”
t0“Arabidopsis Seedlings”
Claim · model-written summary
Extra manganese did not affect growth recovery after 38 days.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- addition of extra manganese (Mn)e1
- Outcome
- growth recoverye0
- Direction
- did not affecte0
- Qualifier
- long-term growth (38 d)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this treatment did not affect the growth recovery after long-term growth (38 d)”
e1“addition of extra manganese (Mn)”
t0“Arabidopsis Seedlings”
Claim · model-written summary
Gene expression patterns on zinc-basal medium resembled zinc-depleted medium with manganese and differed from zinc-depleted medium without manganese.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- gene expression patternse0
- Qualifier
- Zn-depleted medium without Mne0
- Comparator
- Zn-depleted medium with Mne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gene expression patterns of plants on the Zn-basal medium were similar to those on the Zn-depleted medium with Mn, whereas those on the Zn-depleted medium without Mn were different from the others”
t0“Arabidopsis Seedlings”
Claim · model-written summary
Several transcription factors and ROS-related genes were upregulated only without manganese on zinc-depleted medium.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Zn-depleted medium without Mne0
- Outcome
- expression of several transcription factors and reactive oxygen species (ROS)-related genese0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of several transcription factors and reactive oxygen species (ROS)-related genes was upregulated in only plants on the Zn-depleted medium without Mn.”
t0“Arabidopsis Seedlings”
Claim · model-written summary
Root ROS accumulation was higher on zinc-depleted medium without manganese than under other conditions.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- rootse0
- Treatment
- Zn-depleted medium without Mne1
- Outcome
- ROS accumulatione0
- Direction
- highere0
- Comparator
- other conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROS accumulation in the roots grown on this medium was higher than those grown in other conditions”
e1“those on the Zn-depleted medium without Mn”
t0“Arabidopsis Seedlings”
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