Abstract
The overly zinc sensitive Arabidopsis thaliana mutant ozs3 shows reduced growth of the primary root, which is exacerbated by an excess specifically of Zn ions. In addition, ozs3 plants display various subtle developmental phenotypes, such as longer petioles and early flowering. Also, ozs3 seedlings are completely but reversibly growth-arrested when shifted to 4°C. The causal mutation was mapped to a gene encoding a putative substrate-recognition receptor of cullin4 E3 ligases. OZS3 orthologous genes can be found in almost all eukaryotic genomes. Most species from Schizosaccharomyces pombe to Homo sapiens, and including A. thaliana, possess one ortholog. No functional data are available for these genes in any of the multicellular model systems. CRISPR-Cas9-mediated knockout demonstrated that a complete loss of OZS3 function is embryo-lethal, indicating essentiality of OZS3 and its orthologs. The OZS3 protein interacts with the adaptor protein DAMAGED DNA BINDING1 (DDB1) in the nucleus. Thus, it is indeed a member of the large yet poorly characterized family of DDB1-cullin4 associated factors in plants. Mutant phenotypes of ozs3 plants are apparently caused by the weakened DDB1-OZS3 interaction as a result of the exchange of a conserved amino acid near the conserved WDxR motif.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
The ozs3 mutant has reduced primary root growth.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- ozs3e0
- Outcome
- growth of the primary roote0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overly zinc sensitive Arabidopsis thaliana mutant ozs3 shows reduced growth of the primary root”
Claim · model-written summary
Excess zinc exacerbated ozs3 primary root growth reduction.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- ozs3e1
- Treatment
- an excess specifically of Zn ionse0
- Outcome
- growth of the primary roote0
- Direction
- exacerbatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced growth of the primary root, which is exacerbated by an excess specifically of Zn ions”
e1“Arabidopsis thaliana mutant ozs3”
Claim · model-written summary
Ozs3 plants had longer petioles.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- ozs3e0
- Outcome
- petiolese0
- Direction
- longere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ozs3 plants display various subtle developmental phenotypes, such as longer petioles”
e1“Arabidopsis thaliana”
Claim · model-written summary
Ozs3 plants flowered early.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- ozs3e0
- Outcome
- early floweringe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ozs3 plants display various subtle developmental phenotypes, such as longer petioles and early flowering”
e1“Arabidopsis thaliana”
Claim · model-written summary
At 4°C, ozs3 seedling growth was completely but reversibly arrested.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- ozs3e0
- Treatment
- 4°Ce0
- Outcome
- growth-arrestede0
- Qualifier
- completely but reversiblye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ozs3 seedlings are completely but reversibly growth-arrested when shifted to 4°C”
e1“Arabidopsis thaliana”
Claim · model-written summary
The causal mutation mapped to a putative cullin4 E3 substrate receptor gene.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Gene
- OZS3e1
- Outcome
- mapped to a gene encoding a putative substrate-recognition receptor of cullin4 E3 ligasese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The causal mutation was mapped to a gene encoding a putative substrate-recognition receptor of cullin4 E3 ligases”
e1“OZS3 orthologous genes”
e2“Arabidopsis thaliana”
Claim · model-written summary
OZS3 orthologs were found in nearly all eukaryotic genomes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- OZS3e0
- Outcome
- orthologous genes can be found in almost all eukaryotic genomese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OZS3 orthologous genes can be found in almost all eukaryotic genomes”
e1“Arabidopsis thaliana”
Claim · model-written summary
Most surveyed species possess one OZS3 ortholog.
- Taxon
- A. thalianaArabidopsis thalianae0
- Gene
- OZS3e1
- Outcome
- one orthologe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most species from Schizosaccharomyces pombe to Homo sapiens, and including A. thaliana, possess one ortholog”
e1“OZS3 orthologous genes”
Claim · model-written summary
Complete loss of OZS3 function was embryo lethal.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- CRISPR-Cas9-mediated knockoute0
- Gene
- OZS3e0
- Outcome
- embryo-lethale0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRISPR-Cas9-mediated knockout demonstrated that a complete loss of OZS3 function is embryo-lethal”
e1“Arabidopsis thaliana”
Claim · model-written summary
OZS3 interacted with DDB1 in the nucleus.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- OZS3 proteine0
- Outcome
- interacts with the adaptor protein DAMAGED DNA BINDING1 (DDB1)e0
- Setting
- nucleuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OZS3 protein interacts with the adaptor protein DAMAGED DNA BINDING1 (DDB1) in the nucleus”
e1“Arabidopsis thaliana”
Claim · model-written summary
Authors attribute ozs3 phenotypes to weakened DDB1–OZS3 interaction from a conserved amino acid exchange.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- ozs3e0
- Outcome
- Mutant phenotypese0
- Qualifier
- weakened DDB1-OZS3 interaction as a result of the exchange of a conserved amino acid near the conserved WDxR motife0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutant phenotypes of ozs3 plants are apparently caused by the weakened DDB1-OZS3 interaction as a result of the exchange of a conserved amino acid near the conserved WDxR motif”
e1“Arabidopsis thaliana”
The same questions in other papers
zinc atom · growth · Arabidopsis thaliana (thale cress)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.