Abstract
In order to evaluate the effect of tryptophan and glutamine on the tissue culture of upland rice cultivars, serial experiments were conducted using four cultivars: Kusan, Lamsan, Selasi and Siam. Mature seeds from these cultivars were subjected to 4 levels of tryptophan and glutamine on the MSB5 (MS macro elements, B5 micro elements and B5 vitamins) medium. Callus induction results showed a positive effect of tryptophan on all cultivars except Selasi. The optimal tryptophan concentration for callus induction in cultivars Kusan and Siam was 100 µmol, while in Lamsan the optimum was 200 µmol. With the exception of the Lamsan cultivar, incorporation of glutamine generally did not result in the enhancement of callus induction response that incorporation of tryptophan did. Plantlet regeneration frequency was significantly increased when an appropriate level of tryptophan was added to culture media, the optimum being 100 µmol or Kusan, Selasi and Siam, compared to an optimum of 200 µmol for Lamsan. Glutamine did not affect regeneration frequency in any of the cultivars under the conditions tested. In summary, the results showed that tryptophan is a useful additive for upland rice tissue culture.
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Claims 10
Claim · model-written summary
Tryptophan improved callus induction in Kusan, Lamsan and Siam.
- Taxon
- upland rice
Oryza sativat0 - Material
- Kusan, Lamsan, Selasi and Siame1
- Treatment
- tryptophane0
- Outcome
- Callus inductione0
- Direction
- positive effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus induction results showed a positive effect of tryptophan on all cultivars except Selasi”
e1“four cultivars: Kusan, Lamsan, Selasi and Siam”
t0“upland rice”
Claim · model-written summary
Selasi was an exception to the positive tryptophan effect on callus induction.
- Taxon
- upland riceOryza sativat0
- Material
- Selasie0
- Treatment
- tryptophane0
- Outcome
- Callus inductione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus induction results showed a positive effect of tryptophan on all cultivars except Selasi”
t0“upland rice”
Claim · model-written summary
Kusan and Siam had an optimal callus induction tryptophan concentration of 100 µmol.
- Taxon
- upland riceOryza sativat0
- Material
- Kusan and Siame0
- Treatment
- tryptophane0
- Outcome
- optimal tryptophan concentration for callus inductione0
- Value
- 100 µmole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimal tryptophan concentration for callus induction in cultivars Kusan and Siam was 100 µmol”
t0“upland rice”
Claim · model-written summary
Lamsan had an optimal callus induction tryptophan concentration of 200 µmol.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Lamsan the optimum was 200 µmol”
e1“The optimal tryptophan concentration for callus induction”
t0“upland rice”
Claim · model-written summary
Glutamine generally did not enhance callus induction as tryptophan did, except in Lamsan.
- Taxon
- upland riceOryza sativat0
- Material
- Lamsane0
- Treatment
- incorporation of glutaminee0
- Outcome
- callus induction responsee0
- Direction
- did not result in the enhancemente0
- Comparator
- incorporation of tryptophane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With the exception of the Lamsan cultivar, incorporation of glutamine generally did not result in the enhancement of callus induction response that incorporation of tryptophan did”
t0“upland rice”
Claim · model-written summary
Appropriate tryptophan increased plantlet regeneration frequency significantly.
- Taxon
- upland riceOryza sativat0
- Treatment
- tryptophan was added to culture mediae0
- Outcome
- Plantlet regeneration frequencye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plantlet regeneration frequency was significantly increased when an appropriate level of tryptophan was added to culture media”
t0“upland rice”
Claim · model-written summary
Kusan, Selasi and Siam had an optimum 100 µmol tryptophan for regeneration.
- Taxon
- upland riceOryza sativat0
- Material
- Kusan, Selasi and Siame0
- Treatment
- tryptophane1
- Outcome
- Plantlet regeneration frequencye1
- Value
- 100 µmole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the optimum being 100 µmol or Kusan, Selasi and Siam”
e1“Plantlet regeneration frequency was significantly increased when an appropriate level of tryptophan was added to culture media”
t0“upland rice”
Claim · model-written summary
Lamsan had an optimum 200 µmol tryptophan for regeneration.
- Taxon
- upland riceOryza sativat0
- Material
- Lamsane0
- Treatment
- tryptophane1
- Outcome
- Plantlet regeneration frequencye1
- Value
- 200 µmole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“compared to an optimum of 200 µmol for Lamsan”
e1“Plantlet regeneration frequency was significantly increased when an appropriate level of tryptophan was added to culture media”
t0“upland rice”
Claim · model-written summary
Glutamine did not affect regeneration frequency in any cultivar.
- Taxon
- upland riceOryza sativat0
- Treatment
- Glutaminee0
- Outcome
- regeneration frequencye0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutamine did not affect regeneration frequency in any of the cultivars under the conditions tested”
t0“upland rice”
Claim · model-written summary
Authors conclude tryptophan is a useful tissue culture additive.
- Taxon
- upland riceOryza sativae0
- Outcome
- tryptophan is a useful additive for upland rice tissue culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tryptophan is a useful additive for upland rice tissue culture”
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