Abstract
Excessive nickel (Ni) accumulation in edible parts of the plants has become a serious challenge for food security over the past few decades. Therefore, in this study, the role of calcium (Ca2+) signaling in imparting Ni tolerance was investigated in zucchini (Cucurbita pepo L. cv Courgette d’Italie). Exposure of zucchini seedlings to Hogland solution containing 0.28 mmol L−1 Ni(NO3)2 reduced plant growth, the content of chlorophyll and carotenoids and the relative water content (RWC) in leaves, increased Ni accumulation that was accompanied to depletion of the essential bivalent cations and induced oxidative stress and proline accumulation in both shoots and roots. Pretreatment with the nutrient solution containing 15 mmol L−1 calcium chloride (CaCl2), significantly improved zucchini growth and photosynthetic pigment contents and maintained RWC in leaves under both control and Ni stress conditions. Pretreatment with CaCl2 reduced Ni accumulation, modified cation homeostasis, increased the activities of peroxidase and catalase enzymes and lowered Ni-induced accumulation of hydrogen peroxide, malondialdehyde and proline in leaves and roots. Pre-exposure of root with Ca2+ chelator (ethylene glycol tetraacetic acid) and plasma membrane Ca2+ channel blocker (lanthanum chloride) impaired impact of Ca2+ on the aforementioned attributes. Outcomes of this study not only highlight the signaling role of Ca2+ in regulating defensive responses but also suggest an eco- friendly approach for reducing the Ni contamination in plants that ensure food safety.
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Claims 20
Claim · model-written summary
Nickel exposure reduced plant growth.
- Taxon
- zucchini
Cucurbita pepo subsp. pepoe0 - Treatment
- 0.28 mmol L−1 Ni(NO3)2e0
- Outcome
- plant growthe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure of zucchini seedlings to Hogland solution containing 0.28 mmol L−1 Ni(NO3)2 reduced plant growth, the content of chlorophyll and carotenoids and the relative water content (RWC) in leaves, increased Ni accumulation that was accompanied to depletion of the essential bivalent cations and induced oxidative stress and proline accumulation in both shoots and roots”
Claim · model-written summary
Nickel exposure reduced the content of chlorophyll and carotenoids.
- Taxon
- zucchiniCucurbita pepo subsp. pepoe0
- Treatment
- 0.28 mmol L−1 Ni(NO3)2e0
- Outcome
- the content of chlorophyll and carotenoidse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure of zucchini seedlings to Hogland solution containing 0.28 mmol L−1 Ni(NO3)2 reduced plant growth, the content of chlorophyll and carotenoids and the relative water content (RWC) in leaves, increased Ni accumulation that was accompanied to depletion of the essential bivalent cations and induced oxidative stress and proline accumulation in both shoots and roots”
Claim · model-written summary
Nickel exposure reduced relative water content (RWC) in leaves.
- Taxon
- zucchiniCucurbita pepo subsp. pepoe0
- Treatment
- 0.28 mmol L−1 Ni(NO3)2e0
- Outcome
- relative water content (RWC) in leavese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure of zucchini seedlings to Hogland solution containing 0.28 mmol L−1 Ni(NO3)2 reduced plant growth, the content of chlorophyll and carotenoids and the relative water content (RWC) in leaves, increased Ni accumulation that was accompanied to depletion of the essential bivalent cations and induced oxidative stress and proline accumulation in both shoots and roots”
Claim · model-written summary
Nickel exposure increased Ni accumulation.
- Taxon
- zucchiniCucurbita pepo subsp. pepoe0
- Treatment
- 0.28 mmol L−1 Ni(NO3)2e0
- Outcome
- Ni accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure of zucchini seedlings to Hogland solution containing 0.28 mmol L−1 Ni(NO3)2 reduced plant growth, the content of chlorophyll and carotenoids and the relative water content (RWC) in leaves, increased Ni accumulation that was accompanied to depletion of the essential bivalent cations and induced oxidative stress and proline accumulation in both shoots and roots”
Claim · model-written summary
Nickel exposure depletion essential bivalent cations.
- Taxon
- zucchiniCucurbita pepo subsp. pepoe0
- Treatment
- 0.28 mmol L−1 Ni(NO3)2e0
- Outcome
- essential bivalent cationse0
- Direction
- depletione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure of zucchini seedlings to Hogland solution containing 0.28 mmol L−1 Ni(NO3)2 reduced plant growth, the content of chlorophyll and carotenoids and the relative water content (RWC) in leaves, increased Ni accumulation that was accompanied to depletion of the essential bivalent cations and induced oxidative stress and proline accumulation in both shoots and roots”
Claim · model-written summary
Nickel exposure induced oxidative stress.
- Taxon
- zucchiniCucurbita pepo subsp. pepoe0
- Treatment
- 0.28 mmol L−1 Ni(NO3)2e0
- Outcome
- oxidative stresse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure of zucchini seedlings to Hogland solution containing 0.28 mmol L−1 Ni(NO3)2 reduced plant growth, the content of chlorophyll and carotenoids and the relative water content (RWC) in leaves, increased Ni accumulation that was accompanied to depletion of the essential bivalent cations and induced oxidative stress and proline accumulation in both shoots and roots”
Claim · model-written summary
Nickel exposure induced proline accumulation.
- Taxon
- zucchiniCucurbita pepo subsp. pepoe0
- Treatment
- 0.28 mmol L−1 Ni(NO3)2e0
- Outcome
- proline accumulatione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure of zucchini seedlings to Hogland solution containing 0.28 mmol L−1 Ni(NO3)2 reduced plant growth, the content of chlorophyll and carotenoids and the relative water content (RWC) in leaves, increased Ni accumulation that was accompanied to depletion of the essential bivalent cations and induced oxidative stress and proline accumulation in both shoots and roots”
Claim · model-written summary
Calcium chloride pretreatment improved zucchini growth.
- Taxon
- zucchiniCucurbita pepo subsp. pepoe0
- Treatment
- 15 mmol L−1 calcium chloride (CaCl2)e0
- Outcome
- zucchini growthe0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pretreatment with the nutrient solution containing 15 mmol L−1 calcium chloride (CaCl2), significantly improved zucchini growth and photosynthetic pigment contents and maintained RWC in leaves under both control and Ni stress conditions”
Claim · model-written summary
Calcium chloride pretreatment improved photosynthetic pigment contents.
- Taxon
- zucchiniCucurbita pepo subsp. pepoe0
- Treatment
- 15 mmol L−1 calcium chloride (CaCl2)e0
- Outcome
- photosynthetic pigment contentse0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pretreatment with the nutrient solution containing 15 mmol L−1 calcium chloride (CaCl2), significantly improved zucchini growth and photosynthetic pigment contents and maintained RWC in leaves under both control and Ni stress conditions”
Claim · model-written summary
Calcium chloride pretreatment maintained RWC in leaves.
- Taxon
- zucchiniCucurbita pepo subsp. pepoe0
- Treatment
- 15 mmol L−1 calcium chloride (CaCl2)e0
- Outcome
- RWC in leavese0
- Direction
- maintainede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pretreatment with the nutrient solution containing 15 mmol L−1 calcium chloride (CaCl2), significantly improved zucchini growth and photosynthetic pigment contents and maintained RWC in leaves under both control and Ni stress conditions”
Claim · model-written summary
Calcium chloride pretreatment reduced Ni accumulation.
- Taxon
- Cucurbita pepo L.
Cucurbita pepot0 - Treatment
- CaCl2e0
- Outcome
- Ni accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pretreatment with CaCl2 reduced Ni accumulation, modified cation homeostasis, increased the activities of peroxidase and catalase enzymes and lowered Ni-induced accumulation of hydrogen peroxide, malondialdehyde and proline in leaves and roots”
t0“Cucurbita pepo L.”
Claim · model-written summary
Calcium chloride pretreatment modified cation homeostasis.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- CaCl2e0
- Outcome
- cation homeostasise0
- Direction
- modifiede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pretreatment with CaCl2 reduced Ni accumulation, modified cation homeostasis, increased the activities of peroxidase and catalase enzymes and lowered Ni-induced accumulation of hydrogen peroxide, malondialdehyde and proline in leaves and roots”
t0“Cucurbita pepo L.”
Claim · model-written summary
Calcium chloride pretreatment increased activities of peroxidase and catalase enzymes.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- CaCl2e0
- Outcome
- activities of peroxidase and catalase enzymese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pretreatment with CaCl2 reduced Ni accumulation, modified cation homeostasis, increased the activities of peroxidase and catalase enzymes and lowered Ni-induced accumulation of hydrogen peroxide, malondialdehyde and proline in leaves and roots”
t0“Cucurbita pepo L.”
Claim · model-written summary
Calcium chloride pretreatment lowered hydrogen peroxide.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- CaCl2e0
- Outcome
- hydrogen peroxidee0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pretreatment with CaCl2 reduced Ni accumulation, modified cation homeostasis, increased the activities of peroxidase and catalase enzymes and lowered Ni-induced accumulation of hydrogen peroxide, malondialdehyde and proline in leaves and roots”
t0“Cucurbita pepo L.”
Claim · model-written summary
Calcium chloride pretreatment lowered malondialdehyde.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- CaCl2e0
- Outcome
- malondialdehydee0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pretreatment with CaCl2 reduced Ni accumulation, modified cation homeostasis, increased the activities of peroxidase and catalase enzymes and lowered Ni-induced accumulation of hydrogen peroxide, malondialdehyde and proline in leaves and roots”
t0“Cucurbita pepo L.”
Claim · model-written summary
Calcium chloride pretreatment lowered proline.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- CaCl2e0
- Outcome
- prolinee0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pretreatment with CaCl2 reduced Ni accumulation, modified cation homeostasis, increased the activities of peroxidase and catalase enzymes and lowered Ni-induced accumulation of hydrogen peroxide, malondialdehyde and proline in leaves and roots”
t0“Cucurbita pepo L.”
Claim · model-written summary
ethylene glycol tetraacetic acid pre-exposure impaired calcium effects on the measured attributes.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- ethylene glycol tetraacetic acide0
- Outcome
- impact of Ca2+ on the aforementioned attributese0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pre-exposure of root with Ca2+ chelator (ethylene glycol tetraacetic acid) and plasma membrane Ca2+ channel blocker (lanthanum chloride) impaired impact of Ca2+ on the aforementioned attributes”
t0“Cucurbita pepo L.”
Claim · model-written summary
lanthanum chloride pre-exposure impaired calcium effects on the measured attributes.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- lanthanum chloridee0
- Outcome
- impact of Ca2+ on the aforementioned attributese0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pre-exposure of root with Ca2+ chelator (ethylene glycol tetraacetic acid) and plasma membrane Ca2+ channel blocker (lanthanum chloride) impaired impact of Ca2+ on the aforementioned attributes”
t0“Cucurbita pepo L.”
Claim · model-written summary
The authors interpret calcium signaling as regulating defensive responses.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Compound
- Ca2+e0
- Outcome
- defensive responsese0
- Qualifier
- signaling rolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Outcomes of this study not only highlight the signaling role of Ca2+ in regulating defensive responses”
t0“Cucurbita pepo L.”
Claim · model-written summary
The authors suggest calcium treatment as an approach to reduce plant nickel contamination.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Outcome
- reducing the Ni contamination in plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggest an eco- friendly approach for reducing the Ni contamination in plants that ensure food safety”
t0“Cucurbita pepo L.”
The same questions in other papers
nickel atom · nutrient: content · Cucurbita pepoPositive and negative results
1 in the opposite direction
- positive
nickel atom · growth · Cucurbita pepoPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.