Abstract
The objective of the study was to develop an efficient method for shoot regeneration of canola (Brassica napus L.) and to compare the regeneration capacity of different explants on MS medium with several combinations of plant growth regulators. The experiments showed that the morphogenetical potential of canola depends on genotype, primary explant, hormonal structure and concentration of nutrient medium. Cotyledons possessed higher regeneration ability in comparison to hypocotyls and roots. The best regeneration capability was exhibited by the cultivar 'Quantum'. Its frequency with cotyledonary explants reached 68.8% on all used media. Addition of 3 mg/l ABA in nutrient medium considerably increased the regeneration frequency. The highest shoot regeneration (100%), however, took place when cotyledonary explants were cultivated on medium, containing 1.0 mg/l NAA, 8.0 mg/l BAP and 3.0 mg/l ABA. Precultivation of explants on callus induction medium did not affect the shoot regeneration frequency. Vitrification of regenerants was promoted by increasing the auxin NAA or cytokinin BAP, and ABA in the nutrient medium.
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Claims 10
Claim · model-written summary
Canola regeneration potential depended on genotype, explant and medium composition.
- Taxon
- canola
.jpg?width=60)
Brassica napus var. napuse0 - Material
- genotypee0
- Outcome
- morphogenetical potentiale0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The experiments showed that the morphogenetical potential of canola depends on genotype, primary explant, hormonal structure and concentration of nutrient medium.”
Claim · model-written summary
Cotyledons regenerated better than hypocotyls and roots.
- Taxon
- canolaBrassica napus var. napust0
- Organ / stage
- Cotyledonse0
- Outcome
- regeneration abilitye0
- Direction
- highere0
- Comparator
- hypocotyls and rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotyledons possessed higher regeneration ability in comparison to hypocotyls and roots.”
t0“canola”
Claim · model-written summary
Quantum had the best regeneration capability.
- Taxon
- canolaBrassica napus var. napust0
- Material
- Quantume0
- Outcome
- regeneration capabilitye0
- Direction
- beste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best regeneration capability was exhibited by the cultivar 'Quantum'.”
t0“canola”
Claim · model-written summary
Quantum cotyledon explants reached 68.8% regeneration across media.
- Taxon
- canolaBrassica napus var. napust0
- Organ / stage
- cotyledonary explantse0
- Outcome
- frequencye0
- Value
- 68.8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its frequency with cotyledonary explants reached 68.8% on all used media.”
t0“canola”
Claim · model-written summary
Adding ABA increased regeneration frequency.
- Taxon
- canolaBrassica napus var. napust0
- Treatment
- 3 mg/l ABAe0
- Outcome
- regeneration frequencye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of 3 mg/l ABA in nutrient medium considerably increased the regeneration frequency.”
t0“canola”
Claim · model-written summary
Cotyledon explants on the NAA, BAP and ABA medium reached 100% shoot regeneration.
- Taxon
- canolaBrassica napus var. napust0
- Organ / stage
- cotyledonary explantse0
- Treatment
- 1.0 mg/l NAA, 8.0 mg/l BAP and 3.0 mg/l ABAe0
- Outcome
- shoot regeneratione0
- Direction
- higheste0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest shoot regeneration (100%), however, took place when cotyledonary explants were cultivated on medium, containing 1.0 mg/l NAA, 8.0 mg/l BAP and 3.0 mg/l ABA.”
t0“canola”
Claim · model-written summary
Callus induction precultivation did not affect shoot regeneration frequency.
- Taxon
- canolaBrassica napus var. napust0
- Treatment
- Precultivation of explants on callus induction mediume0
- Outcome
- shoot regeneration frequencye0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Precultivation of explants on callus induction medium did not affect the shoot regeneration frequency.”
t0“canola”
Claim · model-written summary
Increasing NAA promoted vitrification of regenerants.
- Taxon
- canolaBrassica napus var. napust0
- Treatment
- increasing the auxin NAAe0
- Outcome
- Vitrification of regenerantse0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitrification of regenerants was promoted by increasing the auxin NAA or cytokinin BAP, and ABA in the nutrient medium.”
t0“canola”
Claim · model-written summary
Increasing BAP promoted vitrification of regenerants.
- Taxon
- canolaBrassica napus var. napust0
- Treatment
- cytokinin BAPe0
- Outcome
- Vitrification of regenerantse0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitrification of regenerants was promoted by increasing the auxin NAA or cytokinin BAP, and ABA in the nutrient medium.”
t0“canola”
Claim · model-written summary
ABA in the medium promoted vitrification of regenerants.
- Taxon
- canolaBrassica napus var. napust0
- Treatment
- ABA in the nutrient mediume0
- Outcome
- Vitrification of regenerantse0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitrification of regenerants was promoted by increasing the auxin NAA or cytokinin BAP, and ABA in the nutrient medium.”
t0“canola”
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