Abstract
In-vitro mutagenesis followed by micropropagation via axillary bud proliferation in shoot tips of guava (Psidium guajava L.) cultivar Safeda was carried out. Shoot tips were irradiated with 15 to 90Gy gamma rays radiations using {sup 60}Co gamma cell source and cultured on MS medium containing 3.0% sucrose, BAP and glutamine. Shoots proliferation was observed 7 weeks after culture initiation. Higher shoot proliferation rates were recorded on MS medium supplemented with 1.0 mgL{sup -1} BAP and 250 mgL{sup -1} glutamine. Rooting was observed on half-strength MS medium supplemented with IAA and IBA. Radio sensitivity test was assessed by determining the percentage of shoot tips survival and shoot proliferation rates. The LD50 (The dose at which 50% of the population killed) was observed on 45 Gy. The doses above 75 Gy were found to be lethal to all explants. Similarly, seeds and bud woods of guava Cv. Safeda were exposed to gamma rays at 0.05 to 0.3 kGy and 20 to 100 Gy respectively using {sup 60}Co gamma source. The LD50 for seeds was determined at 0.19 kGy, and 0.3 kGy was found to be lethal. Seeds irradiated with low doses germinated earlier as compared to those which received higher doses of irradiation. The LD50 for surviving bud woods grafted on the rootstocks in the nursery was determined at 60 Gy. There was constant and continuous reduction in the survival rates of buds as the doses increased. The bud woods of guava Cv. Safeda were more radiosensitive than seeds of the same cultivar. The most favourable doses were established between 0.15 and 0.2 KGy and led to mutations such as: highest number of fruits (40), highest fruit size (59mm) and highest fruit weight of (128.38g). The highest number of seeds (300) and 100 seed-weight (2.29g) were recorded at 0.05KGy while the lowest number of seeds (167) and the lowest 100 seed-weight of 1.56g was observed in the 0.30KGy treatments. (author)
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Claims 17
Claim · model-written summary
The specified BAP and glutamine medium gave higher shoot proliferation.
- Taxon
- guava
Psidium guajavae1 - Material
- Safedae1
- Treatment
- MS medium supplemented with 1.0 mgL{sup -1} BAP and 250 mgL{sup -1} glutaminee0
- Outcome
- shoot proliferation ratese0
- Direction
- Highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher shoot proliferation rates were recorded on MS medium supplemented with 1.0 mgL{sup -1} BAP and 250 mgL{sup -1} glutamine”
e1“guava (Psidium guajava L.) cultivar Safeda”
Claim · model-written summary
Rooting occurred on half-strength MS medium supplemented with IAA and IBA.
- Taxon
- guavaPsidium guajavae1
- Material
- Safedae1
- Treatment
- half-strength MS medium supplemented with IAA and IBAe0
- Outcome
- Rootinge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooting was observed on half-strength MS medium supplemented with IAA and IBA”
e1“guava (Psidium guajava L.) cultivar Safeda”
Claim · model-written summary
The LD50 for guava shoot tips was 45 Gy.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The LD50 (The dose at which 50% of the population killed) was observed on 45 Gy”
e1“guava (Psidium guajava L.) cultivar Safeda”
e2“shoot tips survival and shoot proliferation rates”
Claim · model-written summary
Doses above 75 Gy were lethal to all guava explants.
- Taxon
- guavaPsidium guajavae1
- Treatment
- doses above 75 Gye0
- Outcome
- lethal to all explantse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The doses above 75 Gy were found to be lethal to all explants”
e1“guava (Psidium guajava L.) cultivar Safeda”
Claim · model-written summary
The seed LD50 was 0.19 kGy.
- Taxon
- guavaPsidium guajavae1
- Material
- Safedae1
- Organ / stage
- seedse1
- Outcome
- LD50 for seedse0
- Value
- 0.19 kGye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The LD50 for seeds was determined at 0.19 kGy”
e1“seeds and bud woods of guava Cv. Safeda”
Claim · model-written summary
A 0.3 kGy dose was lethal to guava seeds.
- Taxon
- guavaPsidium guajavae1
- Organ / stage
- seedse1
- Treatment
- 0.3 kGye0
- Outcome
- lethale0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.3 kGy was found to be lethal”
e1“seeds and bud woods of guava Cv. Safeda”
Claim · model-written summary
Seeds given low irradiation doses germinated earlier than seeds given higher doses.
- Taxon
- guavaPsidium guajavae1
- Organ / stage
- Seedse0
- Treatment
- low dosese0
- Outcome
- germinated earliere0
- Direction
- earliere0
- Comparator
- higher doses of irradiatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seeds irradiated with low doses germinated earlier as compared to those which received higher doses of irradiation”
e1“seeds and bud woods of guava Cv. Safeda”
Claim · model-written summary
The LD50 for surviving grafted bud woods was 60 Gy.
- Taxon
- guavaPsidium guajavae1
- Organ / stage
- bud woodse0
- Outcome
- LD50 for surviving bud woodse0
- Value
- 60 Gye0
- Setting
- nurserye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The LD50 for surviving bud woods grafted on the rootstocks in the nursery was determined at 60 Gy”
e1“seeds and bud woods of guava Cv. Safeda”
Claim · model-written summary
Bud survival decreased continuously as irradiation dose increased.
- Taxon
- guavaPsidium guajavae1
- Organ / stage
- budse0
- Treatment
- dosese0
- Outcome
- survival rates of budse0
- Direction
- reductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“constant and continuous reduction in the survival rates of buds as the doses increased”
e1“seeds and bud woods of guava Cv. Safeda”
Claim · model-written summary
Guava bud woods were more radiosensitive than seeds.
- Taxon
- guavaPsidium guajavae0
- Material
- Safedae0
- Organ / stage
- bud woodse0
- Outcome
- radiosensitivee0
- Direction
- moree0
- Comparator
- seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The bud woods of guava Cv. Safeda were more radiosensitive than seeds of the same cultivar”
Claim · model-written summary
The favorable 0.15 to 0.2 KGy doses produced the highest fruit number, 40.
- Taxon
- guavaPsidium guajavae1
- Treatment
- 0.15 and 0.2 KGye0
- Outcome
- number of fruitse0
- Direction
- higheste0
- Value
- 40e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“between 0.15 and 0.2 KGy and led to mutations such as: highest number of fruits (40)”
e1“seeds and bud woods of guava Cv. Safeda”
Claim · model-written summary
The favorable doses produced the highest fruit size, 59mm.
- Taxon
- guavaPsidium guajavae1
- Treatment
- 0.15 and 0.2 KGye0
- Outcome
- fruit sizee0
- Direction
- higheste0
- Value
- 59mme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“between 0.15 and 0.2 KGy and led to mutations such as: highest number of fruits (40), highest fruit size (59mm)”
e1“seeds and bud woods of guava Cv. Safeda”
Claim · model-written summary
The favorable doses produced the highest fruit weight, 128.38g.
- Taxon
- guavaPsidium guajavae1
- Treatment
- 0.15 and 0.2 KGye0
- Outcome
- fruit weighte0
- Direction
- higheste0
- Value
- 128.38ge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“between 0.15 and 0.2 KGy and led to mutations such as: highest number of fruits (40), highest fruit size (59mm) and highest fruit weight of (128.38g)”
e1“seeds and bud woods of guava Cv. Safeda”
Claim · model-written summary
The highest seed number, 300, occurred at 0.05KGy.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest number of seeds (300) and 100 seed-weight (2.29g) were recorded at 0.05KGy”
e1“seeds and bud woods of guava Cv. Safeda”
Claim · model-written summary
The 100 seed-weight was 2.29g at 0.05KGy.
- Taxon
- guavaPsidium guajavae1
- Treatment
- 0.05KGye0
- Outcome
- 100 seed-weighte0
- Value
- 2.29ge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest number of seeds (300) and 100 seed-weight (2.29g) were recorded at 0.05KGy”
e1“seeds and bud woods of guava Cv. Safeda”
Claim · model-written summary
The lowest seed number, 167, occurred at 0.30KGy.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lowest number of seeds (167) and the lowest 100 seed-weight of 1.56g was observed in the 0.30KGy treatments”
e1“seeds and bud woods of guava Cv. Safeda”
Claim · model-written summary
The lowest 100 seed-weight, 1.56g, occurred at 0.30KGy.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lowest number of seeds (167) and the lowest 100 seed-weight of 1.56g was observed in the 0.30KGy treatments”
e1“seeds and bud woods of guava Cv. Safeda”
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