Abstract
L.) seeds, but the mechanism involved in its time-course responses has remained largely unknown. In the present study, the phenotypic differences, ultrastructural changes, physiological characteristics, and proteomic profiles were examined in aged and melatonin-primed seed (with 10 μM melatonin treatment for 12, 24, and 36 h). Thus, 36 h priming (T36) had a better remediation effect on aged seeds, reflecting in the improved germinability and seedlings, relatively intact cell ultrastructures, and enhanced antioxidant capacity. Proteomic analysis revealed 201 differentially abundant proteins between aged and T36 seeds, of which 96 were up-accumulated. In melatonin-primed seeds, the restoration of membrane integrity by improved antioxidant capacity, which was affected by the stimulation of jasmonic acid synthesis via up-accumulation of 12-oxo-phytodienoic acid reductase, might be a candidate mechanism. Moreover, the relatively intact ultrastructures enabled amino acid metabolism and phenylpropanoid biosynthesis, which were closely associated with energy generation through intermediates of pyruvate, phosphoenolpyruvate, fumarate, and α-ketoglutarate, thus providing energy, active amino acids, and secondary metabolites necessary for germination improvement of aged seeds. These findings clarify the time-course related pathways associated with melatonin priming on promoting the germination of aged oat seeds.
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Claims 8
Claim · model-written summary
36 h melatonin priming improved aged seed germinability and seedlings.
- Taxon
- Avena sativa L.
Avena sativat0 - Treatment
- 36 h priming (T36)e0
- Outcome
- germinability and seedlingse0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“36 h priming (T36) had a better remediation effect on aged seeds, reflecting in the improved germinability and seedlings”
t0“Oat (Avena sativa L.) Seeds”
Claim · model-written summary
36 h priming preserved relatively intact cell ultrastructures.
- Taxon
- Avena sativa L.Avena sativat0
- Treatment
- 36 h priming (T36)e0
- Outcome
- relatively intact cell ultrastructurese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“36 h priming (T36) had a better remediation effect on aged seeds, reflecting in the improved germinability and seedlings, relatively intact cell ultrastructures, and enhanced antioxidant capacity”
t0“Oat (Avena sativa L.) Seeds”
Claim · model-written summary
36 h priming enhanced antioxidant capacity.
- Taxon
- Avena sativa L.Avena sativat0
- Treatment
- 36 h priming (T36)e0
- Outcome
- antioxidant capacitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“36 h priming (T36) had a better remediation effect on aged seeds, reflecting in the improved germinability and seedlings, relatively intact cell ultrastructures, and enhanced antioxidant capacity”
t0“Oat (Avena sativa L.) Seeds”
Claim · model-written summary
Proteomic analysis found 201 differentially abundant proteins between aged and T36 seeds.
- Taxon
- Avena sativa L.Avena sativat0
- Outcome
- differentially abundant proteinse0
- Value
- 201e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proteomic analysis revealed 201 differentially abundant proteins between aged and T36 seeds”
t0“Oat (Avena sativa L.) Seeds”
Claim · model-written summary
Ninety-six proteins were up-accumulated.
- Taxon
- Avena sativa L.Avena sativat0
- Outcome
- up-accumulatede0
- Value
- 96e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“of which 96 were up-accumulated”
t0“Oat (Avena sativa L.) Seeds”
Claim · model-written summary
The authors propose antioxidant capacity, stimulated jasmonic acid synthesis and 12-oxo-phytodienoic acid reductase accumulation as a membrane restoration mechanism.
- Taxon
- Avena sativa L.Avena sativat0
- Gene
- 12-oxo-phytodienoic acid reductasee0
- Compound
- jasmonic acide0
- Outcome
- restoration of membrane integritye0
- Qualifier
- might be a candidate mechanisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the restoration of membrane integrity by improved antioxidant capacity, which was affected by the stimulation of jasmonic acid synthesis via up-accumulation of 12-oxo-phytodienoic acid reductase, might be a candidate mechanism”
t0“Oat (Avena sativa L.) Seeds”
Claim · model-written summary
Relatively intact ultrastructures enabled amino acid metabolism and phenylpropanoid biosynthesis.
- Taxon
- Avena sativa L.Avena sativat0
- Outcome
- amino acid metabolism and phenylpropanoid biosynthesise0
- Qualifier
- relatively intact ultrastructurese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the relatively intact ultrastructures enabled amino acid metabolism and phenylpropanoid biosynthesis”
t0“Oat (Avena sativa L.) Seeds”
Claim · model-written summary
Amino acid metabolism and phenylpropanoid biosynthesis were associated with energy generation through listed intermediates.
- Taxon
- Avena sativa L.Avena sativat0
- Compound
- pyruvate, phosphoenolpyruvate, fumarate, and α-ketoglutaratee0
- Outcome
- energy generatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“amino acid metabolism and phenylpropanoid biosynthesis, which were closely associated with energy generation through intermediates of pyruvate, phosphoenolpyruvate, fumarate, and α-ketoglutarate”
t0“Oat (Avena sativa L.) Seeds”
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.