Abstract
THIS STudy was aimed to induce genetic variation toward drought tolerance after treatment with EMS as a mutagen to two maize inbred lines and their single hybrid. The experiment was carried out at Abo-Ghraib Research Station, Baghdad during the spring season of 2019. The experiment included three irrigation intervals 7, 9 and 11, four concentrations of EMS (0, 50, 60, and 70 mM) and three maize genotypes distributed in three replications using randomized complete block design (RCBD) with split-split plot arrangement which included three irrigation intervals 7, 9 and 11 days occupied the main plot, while the sub plots included three maize genotypes, and four EMS concentrations which were distributed in sub-sub plots. Results were revealed significant differences among EMS treatments and irrigation interval treatments. The inbred line 5 in control treatment showed a significant increase in days for both anthesis and silking (70.11 and 71.94) days, respectively. Plants of Nahrain hybrid also showed a super performance in plant height, leaf area, and number of rows ear-1, with a mean of 154.47cm, 746.31cm2 and 17.83 row ear-1, respectively. The irrigation intervals of 9 and 11 days were significantly decreased the mean performance of genotypes, compared to the shortest irrigation interval (7 days) which had highest mean in most traits. None treated plants (0 mM EMS) had a significant effect on the performance of most studied traits, except the anthesis and silking time, which gave the highest values under the concentration 60 mM with 69.11 and 70.48 days, respectively. Hybrid plants interacted significantly with the EMS concentration of 0 mM producing the maximal means of plant height and leaf area (169.56cm and 759.78cm2 respectively). On the other hand, the hybrid was significantly interacted with the shortest irrigation interval in the mean traits of plant's height and leaf area (163.50 cm and 757 cm2, respectively). The triple interaction between hybrids, shortest irrigation interval and EMS concentration of 60 mM had significantly increased leaf area reached 769.67cm2, meanwhile the triple interaction between hybrid plants, shortest irrigation interval, and the EMS concentration of 0 mM recorded the highest value of plant yield (199.67g). It can be concluded from the current study that the genotypes differed in their response to 9 and 11 days irrigation intervals, with a negative effect of the later interval on the vegetative and yield traits of maize compared with shorter irrigation interval.
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Claims 16
Claim · model-written summary
EMS and irrigation treatments differed significantly in measured traits.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Results were revealed significant differences among EMS treatments and irrigation interval treatments.”
t0“MAIZE”
Claim · model-written summary
Untreated inbred line 5 reached anthesis at 70.11 days.
- Material
- inbred line 5e0
- Treatment
- control treatmente0
- Outcome
- days for both anthesis and silkinge0
- Direction
- increasee0
- Value
- 70.11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The inbred line 5 in control treatment showed a significant increase in days for both anthesis and silking (70.11 and 71.94) days, respectively.”
t0“MAIZE”
Claim · model-written summary
Untreated inbred line 5 reached silking at 71.94 days.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The inbred line 5 in control treatment showed a significant increase in days for both anthesis and silking (70.11 and 71.94) days, respectively.”
t0“MAIZE”
Claim · model-written summary
Nahrain hybrid averaged 154.47cm plant height.
Verbatim
e0“Plants of Nahrain hybrid also showed a super performance in plant height, leaf area, and number of rows ear-1, with a mean of 154.47cm, 746.31cm2 and 17.83 row ear-1, respectively.”
Claim · model-written summary
Nahrain hybrid averaged 746.31cm2 leaf area.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Plants of Nahrain hybrid also showed a super performance in plant height, leaf area, and number of rows ear-1, with a mean of 154.47cm, 746.31cm2 and 17.83 row ear-1, respectively.”
t0“MAIZE”
Claim · model-written summary
Nahrain hybrid averaged 17.83 rows per ear.
- Material
- Nahrain hybride0
- Outcome
- number of rows ear-1e0
- Direction
- super performancee0
- Value
- 17.83 row ear-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Plants of Nahrain hybrid also showed a super performance in plant height, leaf area, and number of rows ear-1, with a mean of 154.47cm, 746.31cm2 and 17.83 row ear-1, respectively.”
t0“MAIZE”
Claim · model-written summary
Nine- and eleven-day irrigation intervals reduced most genotype traits versus seven days.
- Treatment
- 9 and 11 dayse0
- Outcome
- mean performance of genotypese0
- Direction
- decreasede0
- Comparator
- 7 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“irrigation intervals of 9 and 11 days were significantly decreased the mean performance of genotypes, compared to the shortest irrigation interval (7 days) which had highest mean in most traits”
t0“MAIZE”
Claim · model-written summary
EMS at 60 mM gave the highest anthesis time, 69.11 days.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“anthesis and silking time, which gave the highest values under the concentration 60 mM with 69.11 and 70.48 days, respectively”
t0“MAIZE”
Claim · model-written summary
EMS at 60 mM gave the highest silking time, 70.48 days.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“anthesis and silking time, which gave the highest values under the concentration 60 mM with 69.11 and 70.48 days, respectively”
t0“MAIZE”
Claim · model-written summary
Hybrid plants without EMS reached maximal height of 169.56cm.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Hybrid plants interacted significantly with the EMS concentration of 0 mM producing the maximal means of plant height and leaf area (169.56cm and 759.78cm2 respectively).”
t0“MAIZE”
Claim · model-written summary
Hybrid plants without EMS reached maximal leaf area of 759.78cm2.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Hybrid plants interacted significantly with the EMS concentration of 0 mM producing the maximal means of plant height and leaf area (169.56cm and 759.78cm2 respectively).”
t0“MAIZE”
Claim · model-written summary
Hybrid plants at the shortest irrigation interval reached 163.50 cm height.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“hybrid was significantly interacted with the shortest irrigation interval in the mean traits of plant’s height and leaf area (163.50 cm and 757 cm2, respectively)”
t0“MAIZE”
Claim · model-written summary
Hybrid plants at the shortest irrigation interval reached 757 cm2 leaf area.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“hybrid was significantly interacted with the shortest irrigation interval in the mean traits of plant’s height and leaf area (163.50 cm and 757 cm2, respectively)”
t0“MAIZE”
Claim · model-written summary
Hybrid plants at shortest irrigation and 60 mM EMS increased leaf area to 769.67cm2.
- Material
- hybridse0
- Treatment
- shortest irrigation interval and EMS concentration of 60 mMe0
- Outcome
- leaf areae0
- Direction
- increasede0
- Value
- 769.67cm2e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“triple interaction between hybrids, shortest irrigation interval and EMS concentration of 60 mM had significantly increased leaf area reached 769.67cm2”
t0“MAIZE”
Claim · model-written summary
Hybrid plants at shortest irrigation and 0 mM EMS yielded the highest 199.67g.
- Material
- hybrid plantse0
- Treatment
- shortest irrigation interval, and the EMS concentration of 0 mMe0
- Outcome
- plant yielde0
- Direction
- higheste0
- Value
- 199.67ge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“triple interaction between hybrid plants, shortest irrigation interval, and the EMS concentration of 0 mM recorded the highest value of plant yield (199.67g)”
t0“MAIZE”
Claim · model-written summary
Genotypes differed in response to longer irrigation intervals, with eleven days harming vegetative and yield traits.
- Material
- genotypese0
- Treatment
- 11 days irrigation intervalse0
- Outcome
- vegetative and yield traitse0
- Direction
- negative effecte0
- Comparator
- shorter irrigation intervale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“genotypes differed in their response to 9 and 11 days irrigation intervals, with a negative effect of the later interval on the vegetative and yield traits of maize compared with shorter irrigation interval”
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